UniProt Accession

 ELOB_HUMAN; Q15370; B7WPD3 

Genbank Protein ID

 L42856; BM700019; AC004493; AC092117; CH471112; CH471112; BC013306; BC065000 

Genbank Nucleotide ID

 AAA75522.1; AAC08452.1; EAW85472.1; EAW85474.1; AAH13306.1; AAH65000.1 

Protein Name

 Transcription elongation factor B polypeptide 2 

Protein Synonyms/Alias

 Elongin 18 kDa subunit; Elongin-B; EloB; RNA polymerase II transcription factor SIII subunit B; SIII p18 

Gene Name

 TCEB2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
19	TTIFTDAKESSTVFE	ubiquitination	[1]
28	SSTVFELKRIVEGIL	ubiquitination	[1]
36	RIVEGILKRPPDEQR	ubiquitination	[1, 2, 3, 4, 5]
46	PDEQRLYKDDQLLDD	ubiquitination	[1, 2, 5]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex). 

Sequence Annotation

 DOMAIN 1 66 Ubiquitin-like.
 MOD_RES 1 1 N-acetylmethionine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; Nucleus; Reference proteome; Transcription; Transcription regulation; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 118 AA 

Protein Sequence

Gene Ontology

 GO:0031462; C:Cul2-RING ubiquitin ligase complex; IDA:UniProtKB.
 GO:0031466; C:Cul5-RING ubiquitin ligase complex; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0031625; F:ubiquitin protein ligase binding; IDA:UniProtKB.
 GO:0050434; P:positive regulation of viral transcription; TAS:Reactome.
 GO:0006461; P:protein complex assembly; TAS:ProtInc.
 GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
 GO:0061418; P:regulation of transcription from RNA polymerase II promoter in response to hypoxia; TAS:Reactome.
 GO:0006368; P:transcription elongation from RNA polymerase II promoter; TAS:Reactome.
 GO:0022415; P:viral reproductive process; TAS:Reactome. 

Interpro

 IPR000626; Ubiquitin.
 IPR019955; Ubiquitin_supergroup. 

Pfam

 PF00240; ubiquitin 

SMART

 SM00213; UBQ 

PROSITE

 PS00299; UBIQUITIN_1
 PS50053; UBIQUITIN_2 

PRINTS