UniProt Accession

 ASNA_MOUSE; O54984; Q3TAQ4 

Genbank Protein ID

 AF039405; AF061177; BC016453; BC083335; AK171690; CH466525 

Genbank Nucleotide ID

 AAB94772.2; AAD15826.2; AAH16453.1; AAH83335.1; BAE42614.1; EDL10985.1 

Protein Name

 ATPase Asna1 

Protein Synonyms/Alias

 Arsenical pump-driving ATPase; Arsenite-stimulated ATPase 

Gene Name

 Asna1 

Gene Synonyms/Alias

 Arsa 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
38	IIEQRSLKWIFVGGK	acetylation	[1]
86	ISDAFDQKFSKVPTK	ubiquitination	[2]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail- anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting (By similarity). 

Sequence Annotation

 NP_BIND 45 52 ATP (By similarity).
 ACT_SITE 74 74 By similarity.
 METAL 289 289 Zinc; shared with dimeric partner (By
 METAL 292 292 Zinc; shared with dimeric partner (By
 BINDING 251 251 ATP (By similarity).
 BINDING 278 278 ATP (By similarity).
 MOD_RES 2 2 N-acetylalanine (By similarity).  

Keyword

 Acetylation; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Endoplasmic reticulum; Hydrolase; Metal-binding; Nucleotide-binding; Nucleus; Reference proteome; Transport; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 348 AA 

Protein Sequence

Gene Ontology

 GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0016887; F:ATPase activity; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0006200; P:ATP catabolic process; IEA:GOC.
 GO:0045048; P:protein insertion into ER membrane; IEA:HAMAP.
 GO:0006810; P:transport; IEA:UniProtKB-KW. 

Interpro

 IPR025723; Anion-transp_ATPase-like_dom.
 IPR016300; ATPase_ArsA/GET3.
 IPR027542; ATPase_ArsA/GET3_euk.
 IPR027417; P-loop_NTPase. 

Pfam

 PF02374; ArsA_ATPase 

SMART

  

PROSITE

  

PRINTS