CPLM-020027

Genbank Nucleotide ID

Target of rapamycin complex 2 subunit MAPKAP1

Protein Synonyms/Alias

TORC2 subunit MAPKAP1; Mitogen-activated protein kinase 2-associated protein 1; Stress-activated map kinase-interacting protein 1; SAPK-interacting protein 1; mSIN1

Homo sapiens (Human)

Position	Peptide	Type	References
166	EYSKFDGKGHVGTTA	ubiquitination	[1]
276	SSPGLTSKESLFVRI	ubiquitination	[1]
501	RADYFAQKQRKLNRR	ubiquitination	[1]

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]

Functional Description

Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient- insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription.

REGION 2 184 Interaction with MAP3K2.
REGION 468 522 Interaction with ATF2.
MOD_RES 2 2 N-acetylalanine.
MOD_RES 510 510 Phosphoserine.

3D-structure; Acetylation; Alternative splicing; Cell membrane; Complete proteome; Cytoplasmic vesicle; Direct protein sequencing; Membrane; Nucleus; Phosphoprotein; Reference proteome; Stress response.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0016023; C:cytoplasmic membrane-bounded vesicle; IEA:UniProtKB-SubCell.
GO:0005829; C:cytosol; TAS:Reactome.
GO:0005794; C:Golgi apparatus; IDA:UniProtKB.
GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0005886; C:plasma membrane; IDA:UniProtKB.
GO:0070300; F:phosphatidic acid binding; IDA:UniProtKB.
GO:0005547; F:phosphatidylinositol-3,4,5-trisphosphate binding; IDA:UniProtKB.
GO:0043325; F:phosphatidylinositol-3,4-bisphosphate binding; IDA:UniProtKB.
GO:0080025; F:phosphatidylinositol-3,5-bisphosphate binding; IDA:UniProtKB.
GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; IDA:UniProtKB.
GO:0017016; F:Ras GTPase binding; IDA:UniProtKB.
GO:0007173; P:epidermal growth factor receptor signaling pathway; TAS:Reactome.
GO:0008543; P:fibroblast growth factor receptor signaling pathway; TAS:Reactome.
GO:0045087; P:innate immune response; TAS:Reactome.
GO:0046580; P:negative regulation of Ras protein signal transduction; IMP:UniProtKB.
GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
GO:0048015; P:phosphatidylinositol-mediated signaling; TAS:Reactome.
GO:0031295; P:T cell costimulation; TAS:Reactome.