CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014629
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Receptor-type tyrosine-protein phosphatase eta 
Protein Synonyms/Alias
 Protein-tyrosine phosphatase eta; R-PTP-eta; HPTP beta-like tyrosine phosphatase; Protein-tyrosine phosphatase receptor type J; R-PTP-J; Susceptibility to colon cancer 1; CD148 
Gene Name
 Ptprj 
Gene Synonyms/Alias
 Byp; Scc1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
915EVSFSQIKPKKSKLIubiquitination[1]
932ENFEAYFKKQQADSNubiquitination[1]
933NFEAYFKKQQADSNCubiquitination[1]
1086TIRDFVVKNMQNSESubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Tyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, FLT3, PDGFRB, MET, RET, KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2. Plays a role in cell adhesion, migration, proliferation and differentiation. Involved in vascular development. May be involved in the mechanism of contact inhibition of cell growth. Regulator of macrophage adhesion and spreading. Positively affects cell-matrix adhesion. Positive regulator of platelet activation and thrombosis. Negative regulator of cell proliferation. Negative regulator of PDGF-stimulated cell migration; through dephosphorylation of PDGFR. Positive regulator of endothelial cell survival, as well as of VEGF-induced SRC and AKT activation; through KDR dephosphorylation. Negative regulator of EGFR signaling pathway; through EGFR dephosphorylation. Enhances the barrier function of epithelial junctions during reassembly. Negatively regulates T-cell receptor (TCR) signaling. Upon T-cell TCR activation, it is up-regulated and excluded from the immunological synapses, while upon T-cell-antigen presenting cells (APC) disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling. 
Sequence Annotation
 DOMAIN 40 130 Fibronectin type-III 1.
 DOMAIN 170 266 Fibronectin type-III 2.
 DOMAIN 267 354 Fibronectin type-III 3.
 DOMAIN 356 440 Fibronectin type-III 4.
 DOMAIN 442 527 Fibronectin type-III 5.
 DOMAIN 529 618 Fibronectin type-III 6.
 DOMAIN 620 712 Fibronectin type-III 7.
 DOMAIN 717 803 Fibronectin type-III 8.
 DOMAIN 942 1199 Tyrosine-protein phosphatase.
 REGION 1140 1146 Substrate binding (By similarity).
 ACT_SITE 1140 1140 Phosphocysteine intermediate (By
 BINDING 1106 1106 Substrate (By similarity).
 BINDING 1184 1184 Substrate (By similarity).
 MOD_RES 912 912 Phosphoserine.
 CARBOHYD 62 62 N-linked (GlcNAc...) (Potential).
 CARBOHYD 78 78 N-linked (GlcNAc...) (Potential).
 CARBOHYD 85 85 N-linked (GlcNAc...) (Potential).
 CARBOHYD 90 90 N-linked (GlcNAc...) (Potential).
 CARBOHYD 110 110 N-linked (GlcNAc...) (Potential).
 CARBOHYD 114 114 N-linked (GlcNAc...) (Potential).
 CARBOHYD 145 145 N-linked (GlcNAc...).
 CARBOHYD 164 164 N-linked (GlcNAc...) (Potential).
 CARBOHYD 173 173 N-linked (GlcNAc...) (Potential).
 CARBOHYD 182 182 N-linked (GlcNAc...) (Potential).
 CARBOHYD 198 198 N-linked (GlcNAc...) (Potential).
 CARBOHYD 207 207 N-linked (GlcNAc...) (Potential).
 CARBOHYD 244 244 N-linked (GlcNAc...) (Potential).
 CARBOHYD 253 253 N-linked (GlcNAc...) (Potential).
 CARBOHYD 267 267 N-linked (GlcNAc...) (Potential).
 CARBOHYD 278 278 N-linked (GlcNAc...) (Potential).
 CARBOHYD 313 313 N-linked (GlcNAc...).
 CARBOHYD 317 317 N-linked (GlcNAc...).
 CARBOHYD 333 333 N-linked (GlcNAc...) (Potential).
 CARBOHYD 366 366 N-linked (GlcNAc...) (Potential).
 CARBOHYD 379 379 N-linked (GlcNAc...) (Potential).
 CARBOHYD 398 398 N-linked (GlcNAc...) (Potential).
 CARBOHYD 403 403 N-linked (GlcNAc...) (Potential).
 CARBOHYD 437 437 N-linked (GlcNAc...) (Potential).
 CARBOHYD 452 452 N-linked (GlcNAc...) (Potential).
 CARBOHYD 488 488 N-linked (GlcNAc...) (Potential).
 CARBOHYD 506 506 N-linked (GlcNAc...) (Potential).
 CARBOHYD 538 538 N-linked (GlcNAc...).
 CARBOHYD 572 572 N-linked (GlcNAc...) (Potential).
 CARBOHYD 576 576 N-linked (GlcNAc...) (Potential).
 CARBOHYD 662 662 N-linked (GlcNAc...) (Potential).
 CARBOHYD 668 668 N-linked (GlcNAc...) (Potential).
 CARBOHYD 685 685 N-linked (GlcNAc...) (Potential).
 CARBOHYD 691 691 N-linked (GlcNAc...) (Potential).
 CARBOHYD 725 725 N-linked (GlcNAc...) (Potential).
 CARBOHYD 811 811 N-linked (GlcNAc...) (Potential).
 CARBOHYD 838 838 N-linked (GlcNAc...) (Potential).  
Keyword
 Cell junction; Cell membrane; Cell projection; Complete proteome; Glycoprotein; Hydrolase; Membrane; Phosphoprotein; Protein phosphatase; Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1238 AA 
Protein Sequence
MKPAARETRT PPRSPGLRWA LLPLLLLLRQ GQVLCAGAAP NPIFDIEAVV SPTSVLLTWK 60
HNDSGASECR IENKMESNLT FPVKNQTSCN ITGLSPGTSY TFSIISVTTN ETLNKTITTE 120
PWPVSDLHVT SVGVTQARLT WSNANGTASY RMLIEELTTH SSVNISGLKP GTNNSFAFPE 180
SNETQADFAV AEEVPDANGT KRIPVTNLSQ LHKNSLVSVD PPSGQDPSLT EILLTDLKPD 240
TQYNATIYSQ AANGTEGQPR NKVFKTNSTQ VSDVRAMNIS ASSMTLTWKS NYDGSRTSIV 300
YKIHVAGGTH SVNQTVNKTE AIILGLSSST LYNITVHPFL GQTEGTPGFL QVYTSPDQVS 360
DFRVTNVSTR AIGLAWRSND SKSFEIFIKQ DGGEKHRNAS TGNQSYMVED LKPGTSYHFE 420
IIPRGPDGTE GLSSTVNGST DPSAVTDIRV VNISTTEMQL EWQNTDDASG YTYHLVLESK 480
SGSIIRTNSS QKWITVGSLT PGTLYNVTIF PEVDQIQGIS NSITQYTRPS SVSHIEVNTT 540
TTTAAIRWKN EDAASASYAY SVLILKTGDG SNVTSNFTKD PSILIPELIP GVSYTVKILT 600
QVGDGTTSLV PGWNLFCTEP EPVTSFHCEV VPKEPALVLK WACPFGMYTG FELGVRSDSW 660
DNMTRLENCT SDDDTECRTE VAYLNFSTSY NISIATLSCG KMALPAQNIC TTGITDPPTP 720
DGSPNITSVS HNSVKVKFSG FEASHGPIKA YAVILTTGEA AQPSADVLKY TYEDFKRGAS 780
DTYVTYLIRI EEKGQSQGLS EVLNYEIDVG NQSTTLGYYN GRLEPLGSYR ACVAGFTNIT 840
YNLQNDGLIN GDESYVSFSP YSEAVFLPQD PGVICGAVFG CIFGALAITA VGGFIFWRKK 900
RTDAKNNEVS FSQIKPKKSK LIRVENFEAY FKKQQADSNC GFAEEYEDLK LIGISLPKYT 960
AEIAENRGKN RYNNVLPYDI SRVKLSVQTH STDDYINANY MPGYHSKKDF IATQGPLPNT 1020
LKDFWRMVWE KNVYAIVMLT KCVEQGRTKC EEYWPSKQAQ DYGDITVAMT SEVVLPEWTI 1080
RDFVVKNMQN SESHPLRQFH FTSWPDHGVP DTTDLLINFR YLVRDYMKQI PPESPILVHC 1140
SAGVGRTGTF IAIDRLIYQI ENENTVDVYG IVYDLRMHRP LMVQTEDQYV FLNQCVLDII 1200
RAQKDSKVDL IYQNTTAMTI YENLEPVSMF GKTNGYIA 1238 
Gene Ontology
 GO:0009986; C:cell surface; IEA:Compara.
 GO:0005911; C:cell-cell junction; ISS:UniProtKB.
 GO:0001772; C:immunological synapse; IDA:UniProtKB.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0032587; C:ruffle membrane; IDA:UniProtKB.
 GO:0004725; F:protein tyrosine phosphatase activity; IMP:UniProtKB.
 GO:0007596; P:blood coagulation; IMP:UniProtKB.
 GO:0007507; P:heart development; IMP:MGI.
 GO:0030308; P:negative regulation of cell growth; IEA:Compara.
 GO:0030336; P:negative regulation of cell migration; IDA:UniProtKB.
 GO:0008285; P:negative regulation of cell proliferation; IMP:UniProtKB.
 GO:0042059; P:negative regulation of epidermal growth factor receptor signaling pathway; ISS:UniProtKB.
 GO:0043407; P:negative regulation of MAP kinase activity; IDA:UniProtKB.
 GO:0010642; P:negative regulation of platelet-derived growth factor receptor signaling pathway; IDA:UniProtKB.
 GO:0051898; P:negative regulation of protein kinase B signaling cascade; ISS:UniProtKB.
 GO:2000272; P:negative regulation of receptor activity; IMP:UniProtKB.
 GO:0050860; P:negative regulation of T cell receptor signaling pathway; IDA:UniProtKB.
 GO:0043116; P:negative regulation of vascular permeability; IEA:Compara.
 GO:0048008; P:platelet-derived growth factor receptor signaling pathway; ISS:UniProtKB.
 GO:0050918; P:positive chemotaxis; IEA:Compara.
 GO:0051894; P:positive regulation of focal adhesion assembly; ISS:UniProtKB.
 GO:0010759; P:positive regulation of macrophage chemotaxis; IDA:UniProtKB.
 GO:0010572; P:positive regulation of platelet activation; IMP:UniProtKB.
 GO:0051897; P:positive regulation of protein kinase B signaling cascade; IEA:Compara.
 GO:0001570; P:vasculogenesis; IMP:MGI. 
Interpro
 IPR003961; Fibronectin_type3.
 IPR013783; Ig-like_fold.
 IPR000387; Tyr/Dual-sp_Pase.
 IPR016130; Tyr_Pase_AS.
 IPR000242; Tyr_Pase_rcpt/non-rcpt. 
Pfam
 PF00041; fn3
 PF00102; Y_phosphatase 
SMART
 SM00060; FN3
 SM00194; PTPc 
PROSITE
 PS50853; FN3
 PS00383; TYR_PHOSPHATASE_1
 PS50056; TYR_PHOSPHATASE_2
 PS50055; TYR_PHOSPHATASE_PTP 
PRINTS
 PR00700; PRTYPHPHTASE.