UniProt Accession

 CAN2_RAT; Q07009 

Genbank Protein ID

 L09120; BC065306 

Genbank Nucleotide ID

 AAA16327.1; AAH65306.1 

Protein Name

 Calpain-2 catalytic subunit 

Protein Synonyms/Alias

 Calcium-activated neutral proteinase 2; CANP 2; Calpain M-type; Calpain-2 large subunit; Millimolar-calpain; M-calpain 

Gene Name

 Capn2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
7	*MAGIAMKLAKDREA	acetylation	[1]
280	ESSGSLQKLIRIRNP	acetylation	[1]
609	TKIQKYQKIYREIDV	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. 

Sequence Annotation

 DOMAIN 45 344 Calpain catalytic.
 DOMAIN 572 605 EF-hand 1.
 DOMAIN 602 637 EF-hand 2.
 DOMAIN 667 700 EF-hand 3.
 REGION 345 514 Domain III.
 REGION 515 529 Linker.
 REGION 530 700 Domain IV.
 ACT_SITE 105 105
 ACT_SITE 262 262
 ACT_SITE 286 286
 METAL 89 89 Calcium 3; via carbonyl oxygen.
 METAL 91 91 Calcium 3; via carbonyl oxygen.
 METAL 96 96 Calcium 3.
 METAL 175 175 Calcium 3.
 METAL 229 229 Calcium 2.
 METAL 230 230 Calcium 2.
 METAL 292 292 Calcium 4.
 METAL 299 299 Calcium 4.
 METAL 319 319 Calcium 4; via carbonyl oxygen.
 METAL 323 323 Calcium 4; via carbonyl oxygen.
 METAL 542 542 Calcium 5; via carbonyl oxygen.
 METAL 545 545 Calcium 5.
 METAL 547 547 Calcium 5; via carbonyl oxygen.
 METAL 552 552 Calcium 5.
 METAL 585 585 Calcium 6.
 METAL 587 587 Calcium 6.
 METAL 589 589 Calcium 6; via carbonyl oxygen.
 METAL 591 591 Calcium 6; via carbonyl oxygen.
 METAL 596 596 Calcium 6.
 METAL 615 615 Calcium 7.
 METAL 617 617 Calcium 7.
 METAL 619 619 Calcium 7; via carbonyl oxygen.
 METAL 621 621 Calcium 7; via carbonyl oxygen.
 METAL 626 626 Calcium 7.
 METAL 658 658 Calcium 1.
 METAL 661 661 Calcium 1.  

Keyword

 3D-structure; Calcium; Cell membrane; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Membrane; Metal-binding; Protease; Reference proteome; Repeat; Thiol protease. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 700 AA 

Protein Sequence

Gene Ontology

 GO:0000785; C:chromatin; IEA:Compara.
 GO:0005737; C:cytoplasm; TAS:RGD.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0005509; F:calcium ion binding; IDA:RGD.
 GO:0004198; F:calcium-dependent cysteine-type endopeptidase activity; IDA:RGD.
 GO:0008092; F:cytoskeletal protein binding; IDA:RGD.
 GO:0001824; P:blastocyst development; IEA:Compara.
 GO:0007520; P:myoblast fusion; IEA:Compara.
 GO:0016540; P:protein autoprocessing; IDA:RGD.
 GO:0006508; P:proteolysis; IDA:RGD.
 GO:0001666; P:response to hypoxia; IEP:RGD. 

Interpro

 IPR022684; Calpain_cysteine_protease.
 IPR022682; Calpain_domain_III.
 IPR022683; Calpain_III.
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR000169; Pept_cys_AS.
 IPR001300; Peptidase_C2_calpain_cat. 

Pfam

 PF01067; Calpain_III
 PF00648; Peptidase_C2 

SMART

 SM00720; calpain_III
 SM00230; CysPc
 SM00054; EFh 

PROSITE

 PS50203; CALPAIN_CAT
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS00640; THIOL_PROTEASE_ASN
 PS00139; THIOL_PROTEASE_CYS
 PS00639; THIOL_PROTEASE_HIS 

PRINTS

 PR00704; CALPAIN.