UniProt Accession

 CLP1_RAT; Q5PQL4 

Genbank Protein ID

 CH473949; BC087130 

Genbank Nucleotide ID

 EDL79312.1; AAH87130.1 

Protein Name

 Polyribonucleotide 5'-hydroxyl-kinase Clp1 

Protein Synonyms/Alias

 Polyadenylation factor Clp1; Polynucleotide kinase Clp1; Pre-mRNA cleavage complex II protein Clp1 

Gene Name

 Clp1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
267	ERLYNELKRDLPHFV	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Polynucleotide kinase that can phosphorylate the 5'- hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Appears to have roles in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex. Phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA- induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing (By similarity). 

Sequence Annotation

 NP_BIND 124 129 ATP (By similarity).
 BINDING 22 22 ATP (By similarity).
 BINDING 62 62 ATP; via carbonyl oxygen (By similarity).  

Keyword

 ATP-binding; Complete proteome; Kinase; Magnesium; Manganese; mRNA processing; Nickel; Nucleotide-binding; Nucleus; Reference proteome; Transferase; tRNA processing. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 425 AA 

Protein Sequence

Gene Ontology

 GO:0000214; C:tRNA-intron endonuclease complex; ISS:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0046404; F:ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity; IEA:EC.
 GO:0051736; F:ATP-dependent polyribonucleotide 5'-hydroxyl-kinase activity; ISS:UniProtKB.
 GO:0051733; F:polydeoxyribonucleotide kinase activity; ISS:UniProtKB.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0035087; P:siRNA loading onto RISC involved in RNA interference; ISS:UniProtKB.
 GO:0030423; P:targeting of mRNA for destruction involved in RNA interference; ISS:UniProtKB.
 GO:0006388; P:tRNA splicing, via endonucleolytic cleavage and ligation; ISS:UniProtKB. 

Interpro

 IPR027417; P-loop_NTPase.
 IPR010655; Pre-mRNA_cleavage_cplxII_Clp1. 

Pfam

 PF06807; Clp1 

SMART

  

PROSITE

  

PRINTS