CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013267
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Coatomer subunit gamma-1 
Protein Synonyms/Alias
 Gamma-1-coat protein; Gamma-1-COP 
Gene Name
 Copg1 
Gene Synonyms/Alias
 Copg 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
217AVSKMISKFTRHGLKacetylation[1]
538YLNVLEQKQKALNAGubiquitination[2]
540NVLEQKQKALNAGYIubiquitination[2]
572YTLEPSEKPFDLKSVacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023
Functional Description
 The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte triglyceride lipase (PNPLA2) with the lipid droplet surface to mediate lipolysis (By similarity). 
Sequence Annotation
 REPEAT 64 101 HEAT 1.
 REPEAT 283 320 HEAT 2.
 REPEAT 322 355 HEAT 3.
 REPEAT 356 392 HEAT 4.
 REGION 609 874 Interaction with ZNF289/ARFGAP2 (By  
Keyword
 Complete proteome; Cytoplasm; Cytoplasmic vesicle; ER-Golgi transport; Golgi apparatus; Membrane; Protein transport; Reference proteome; Repeat; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 874 AA 
Protein Sequence
MLKKFDKKDE ESGGGSNPLQ HLEKSAVLQE ARVFNETPIN PRKCAHILTK ILYLINQGEH 60
LGTTEATEAF FAMTKLFQSN DPTLRRMCYL TIKEMSCIAE DVIIVTSSLT KDMTGKEDNY 120
RGPAVRALCQ ITDSTMLQAV ERYMKQAIVD KVPSVSSSAL VSSLHLLKCS FDVVKRWVNE 180
AQEAASSDNI MVQYHALGLL YHVRKNDRLA VSKMISKFTR HGLKSPFAYC MMIRVASKQL 240
EEEDGSRDSP LFDFIESCLR NKHEMVVYEA ASAIVNLPGC SAKELAPAVS VLQLFCSSPK 300
AALRYAAVRT LNKVAMKHPS AVTACNLDLE NLVTDSNRSI ATLAITTLLK TGSESSIDRL 360
MKQISSFMSE ISDEFKVVVV QAISALCQKY PRKHAVLMNF LFTMLREEGG FEYKRAIVDC 420
IISIIEENSE SKETGLSHLC EFIEDCEFTV LATRILHLLG QEGPKTNNPS KYIRFIYNRV 480
VLEHEEVRAG AVSALAKFGA QNEEMLPSIL VLLKRCVMDD DNEVRDRATF YLNVLEQKQK 540
ALNAGYILNG LTVSIPGLEK ALQQYTLEPS EKPFDLKSVP LATTPMTEQR PESTSTAAVK 600
QPEKVAATRQ EIFQEQLAAV PEFQGLGPLF KSSPEPVALT ESETEYVIRC TKHTFSDHLV 660
FQFDCTNTLN DQTLENVTVQ MEPTEAYEVL SYVPVRSLPY NQPGTCYTLV ALPKEDPTAV 720
ACTFSCVMKF TVKDCDPNTG EIDEEGYEDE YVLEDLEVTV ADHIQKVMKV NFEAAWDEVG 780
DEFEKEETFT LSTIKTLEEA VGNIVKFLGM HPCERSDKVP ENKNTHTLLL AGVFRGGHDI 840
LVRSRLLLLD TVTMQVTARS SEELPVDIIL ASVG 874 
Gene Ontology
 GO:0030126; C:COPI vesicle coat; IEA:InterPro.
 GO:0000139; C:Golgi membrane; IDA:BHF-UCL.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0005198; F:structural molecule activity; IEA:InterPro.
 GO:0051683; P:establishment of Golgi localization; IMP:BHF-UCL.
 GO:0006886; P:intracellular protein transport; IEA:InterPro.
 GO:0072384; P:organelle transport along microtubule; IMP:BHF-UCL.
 GO:0016192; P:vesicle-mediated transport; IEA:UniProtKB-KW. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR002553; Clathrin/coatomer_adapt-like_N.
 IPR015873; Clathrin_a/coatomer_app_sub_C.
 IPR009028; Coatomer/calthrin_app_sub_C.
 IPR013041; Coatomer/clathrin_app_Ig-like.
 IPR017106; Coatomer_gsu.
 IPR014863; Coatomer_gsu_app.
 IPR013040; Coatomer_gsu_app_Ig-like-sub. 
Pfam
 PF01602; Adaptin_N
 PF08752; Gamma-COP 
SMART
  
PROSITE
 PS50077; HEAT_REPEAT 
PRINTS