CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005872
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glycylpeptide N-tetradecanoyltransferase 1 
Protein Synonyms/Alias
 Myristoyl-CoA:protein N-myristoyltransferase 1; NMT 1; Type I N-myristoyltransferase; Peptide N-myristoyltransferase 1 
Gene Name
 NMT1 
Gene Synonyms/Alias
 NMT 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
80SAQDQPVKMNSLPAEubiquitination[1, 2]
94ERIQEIQKAIELFSVubiquitination[2, 3, 4, 5]
325MTMQRTMKLYRLPETubiquitination[2]
334YRLPETPKTAGLRPMubiquitination[2]
445ALVLAKMKGFDVFNAubiquitination[2]
464ENKTFLEKLKFGIGDubiquitination[2]
482QYYLYNWKCPSMGAEubiquitination[6]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
 Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins. 
Sequence Annotation
 MOD_RES 31 31 Phosphoserine (By similarity).
 MOD_RES 47 47 Phosphoserine.  
Keyword
 3D-structure; Acyltransferase; Alternative splicing; Complete proteome; Cytoplasm; Phosphoprotein; Polymorphism; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 496 AA 
Protein Sequence
MADESETAVK PPAPPLPQMM EGNGNGHEHC SDCENEEDNS YNRGGLSPAN DTGAKKKKKK 60
QKKKKEKGSE TDSAQDQPVK MNSLPAERIQ EIQKAIELFS VGQGPAKTME EASKRSYQFW 120
DTQPVPKLGE VVNTHGPVEP DKDNIRQEPY TLPQGFTWDA LDLGDRGVLK ELYTLLNENY 180
VEDDDNMFRF DYSPEFLLWA LRPPGWLPQW HCGVRVVSSR KLVGFISAIP ANIHIYDTEK 240
KMVEINFLCV HKKLRSKRVA PVLIREITRR VHLEGIFQAV YTAGVVLPKP VGTCRYWHRS 300
LNPRKLIEVK FSHLSRNMTM QRTMKLYRLP ETPKTAGLRP METKDIPVVH QLLTRYLKQF 360
HLTPVMSQEE VEHWFYPQEN IIDTFVVENA NGEVTDFLSF YTLPSTIMNH PTHKSLKAAY 420
SFYNVHTQTP LLDLMSDALV LAKMKGFDVF NALDLMENKT FLEKLKFGIG DGNLQYYLYN 480
WKCPSMGAEK VGLVLQ 496 
Gene Ontology
 GO:0015629; C:actin cytoskeleton; IDA:HPA.
 GO:0030054; C:cell junction; IDA:HPA.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0003824; F:catalytic activity; TAS:ProtInc.
 GO:0004379; F:glycylpeptide N-tetradecanoyltransferase activity; IEA:EC.
 GO:0097193; P:intrinsic apoptotic signaling pathway; TAS:Reactome.
 GO:0006499; P:N-terminal protein myristoylation; NAS:UniProtKB.
 GO:1900740; P:positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway; TAS:Reactome.
 GO:0009249; P:protein lipoylation; TAS:ProtInc.
 GO:0022415; P:viral reproductive process; TAS:Reactome. 
Interpro
 IPR016181; Acyl_CoA_acyltransferase.
 IPR000903; MyristoylCoA_TrFase.
 IPR022677; MyristoylCoA_TrFase_C.
 IPR022678; MyristoylCoA_TrFase_CS.
 IPR022676; MyristoylCoA_TrFase_N. 
Pfam
 PF01233; NMT
 PF02799; NMT_C 
SMART
  
PROSITE
 PS00975; NMT_1
 PS00976; NMT_2 
PRINTS