CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016345
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 RAS protein activator like-3 
Protein Synonyms/Alias
  
Gene Name
 RASAL3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
33HTGGGGEKAAGGFRWubiquitination[1]
459PALPAQAKEELAAAMubiquitination[2]
507RENTLATKAIDEYMKubiquitination[2]
545DCEVDPSKCPASELPubiquitination[2]
Reference
 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 May function as a Ras GTPase-activating protein (By similarity). 
Sequence Annotation
 DOMAIN 197 293 PH.
 DOMAIN 306 384 C2.
 DOMAIN 458 650 Ras-GAP.
 MOD_RES 18 18 Phosphoserine.
 MOD_RES 164 164 Phosphoserine.
 MOD_RES 167 167 Phosphoserine.
 MOD_RES 231 231 Phosphoserine.  
Keyword
 Alternative splicing; Coiled coil; Complete proteome; GTPase activation; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1011 AA 
Protein Sequence
MDPPSPSRTS QTQPTATSPL TSYRWHTGGG GEKAAGGFRW GRFAGWGRAL SHQEPMVSTQ 60
PAPRSIFRRV LSAPPKESRT SRLRLSKALW GRHKNPPPEP DPEPEQEAPE LEPEPELEPP 120
TPQIPEAPTP NVPVWDIGGF TLLDGKLVLL GGEEEGPRRP RVGSASSEGS IHVAMGNFRD 180
PDRMPGKTEP ETAGPNQVHN VRGLLKRLKE KKKARLEPRD GPPSALGSRE SLATLSELDL 240
GAERDVRIWP LHPSLLGEPH CFQVTWTGGS RCFSCRSAAE RDRWIEDLRR QFQPTQDNVE 300
REETWLSVWV HEAKGLPRAA AGAPGVRAEL WLDGALLART APRAGPGQLF WAERFHFEAL 360
PPARRLSLRL RGLGPGSAVL GRVALALEEL DAPRAPAAGL ERWFPLLGAP AGAALRARIR 420
ARRLRVLPSE RYKELAEFLT FHYARLCGAL EPALPAQAKE ELAAAMVRVL RATGRAQALV 480
TDLGTAELAR CGGREALLFR ENTLATKAID EYMKLVAQDY LQETLGQVVR RLCASTEDCE 540
VDPSKCPASE LPEHQARLRN SCEEVFETII HSYDWFPAEL GIVFSSWREA CKERGSEVLG 600
PRLVCASLFL RLLCPAILAP SLFGLAPDHP APGPARTLTL IAKVIQNLAN RAPFGEKEAY 660
MGFMNSFLEE HGPAMQCFLD QVAMVDVDAA PSGYQGSGDL ALQLAVLHAQ LCTIFAELDQ 720
TTRDTLEPLP TILRAIEEGQ PVLVSVPMRL PLPPAQVHSS LSAGEKPGFL APRDLPKHTP 780
LISKSQSLRS VRRSESWARP RPDEERPLRR PRPVQRTQSV PVRRPARRRQ SAGPWPRPKG 840
SLSMGPAPRA RPWTRDSASL PRKPSVPWQR QMDQPQDRNQ ALGTHRPVNK LAELQCEVAA 900
LREEQKVLSR LVESLSTQIR ALTEQQEQLR GQLQDLDSRL RAGSSEFDSE HNLTSNEGHS 960
LKNLEHRLNE MERTQAQLRD AVQSLQLSPR TRGSWSQPQP LKAPCLNGDT T 1011 
Gene Ontology
 GO:0005737; C:cytoplasm; IBA:RefGenome.
 GO:0031235; C:intrinsic to internal side of plasma membrane; IBA:RefGenome.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0005099; F:Ras GTPase activator activity; IBA:RefGenome.
 GO:0046580; P:negative regulation of Ras protein signal transduction; IBA:RefGenome.
 GO:0007165; P:signal transduction; IEA:InterPro. 
Interpro
 IPR008973; C2_Ca/lipid-bd_dom_CaLB.
 IPR001849; Pleckstrin_homology.
 IPR001936; RasGAP.
 IPR023152; RasGAP_CS.
 IPR008936; Rho_GTPase_activation_prot. 
Pfam
 PF00616; RasGAP 
SMART
 SM00233; PH
 SM00323; RasGAP 
PROSITE
 PS50004; C2
 PS50003; PH_DOMAIN
 PS00509; RAS_GTPASE_ACTIV_1
 PS50018; RAS_GTPASE_ACTIV_2 
PRINTS