CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022024
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Inner centromere protein 
Protein Synonyms/Alias
  
Gene Name
 INCENP 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
51MFTREFSKEPELMPKubiquitination[1, 2, 3]
321SPQVLAQKYSLVAKQubiquitination[1, 3]
327QKYSLVAKQESVVRRubiquitination[4]
602KKKQIEQKFAQIDEKacetylation[5]
602KKKQIEQKFAQIDEKubiquitination[1, 3, 6]
609KFAQIDEKTEKAKEEacetylation[5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Probably acts through association with AURKB or AURKC. Seems to bind directly to microtubules. Controls the kinetochore localization of BUB1. 
Sequence Annotation
 MOD_RES 119 119 Phosphoserine.
 MOD_RES 143 143 Phosphoserine.
 MOD_RES 148 148 Phosphoserine.
 MOD_RES 195 195 Phosphothreonine.
 MOD_RES 197 197 Phosphoserine.
 MOD_RES 199 199 Phosphothreonine.
 MOD_RES 213 213 Phosphothreonine.
 MOD_RES 214 214 Phosphoserine.
 MOD_RES 239 239 Phosphothreonine.
 MOD_RES 263 263 Phosphoserine.
 MOD_RES 269 269 Phosphoserine.
 MOD_RES 275 275 Phosphoserine.
 MOD_RES 292 292 Phosphothreonine.
 MOD_RES 296 296 Phosphoserine.
 MOD_RES 306 306 Phosphoserine.
 MOD_RES 312 312 Phosphoserine.
 MOD_RES 314 314 Phosphoserine.
 MOD_RES 330 330 Phosphoserine.
 MOD_RES 400 400 Phosphoserine.
 MOD_RES 406 406 Phosphothreonine.
 MOD_RES 446 446 Phosphoserine.
 MOD_RES 476 476 Phosphoserine.
 MOD_RES 510 510 Phosphoserine.
 MOD_RES 514 514 Phosphoserine.
 MOD_RES 828 828 Phosphoserine.
 MOD_RES 831 831 Phosphoserine.
 MOD_RES 832 832 Phosphothreonine.
 MOD_RES 892 892 Phosphothreonine; by AURKB.
 MOD_RES 893 893 Phosphoserine; by AURKB.
 MOD_RES 894 894 Phosphoserine; by AURKB.
 MOD_RES 899 899 Phosphoserine.
 MOD_RES 914 914 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Kinetochore; Microtubule; Mitosis; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 918 AA 
Protein Sequence
MGTTAPGPIH LLELCDQKLM EFLCNMDNKD LVWLEEIQEE AERMFTREFS KEPELMPKTP 60
SQKNRRKKRR ISYVQDENRD PIRRRLSRRK SRSSQLSSRR LRSKDSVEKL ATVVGENGSV 120
LRRVTRAAAA AAAATMALAA PSSPTPESPT MLTKKPEDNH TQCQLVPVVE IGISERQNAE 180
QHVTQLMSTE PLPRTLSPTP ASATAPTSQG IPTSDEESTP KKSKARILES ITVSSLMATP 240
QDPKGQGVGT GRSASKLRIA QVSPGPRDSP AFPDSPWRER VLAPILPDNF STPTGSRTDS 300
QSVRHSPIAP SSPSPQVLAQ KYSLVAKQES VVRRASRRLA KKTAEEPAAS GRIICHSYLE 360
RLLNVEVPQK VGSEQKEPPE EAEPVAAAEP EVPENNGNNS WPHNDTEIAN STPNPKPAAS 420
SPETPSAGQQ EAKTDQADGP REPPQSARRK RSYKQAVSEL DEEQHLEDEE LQPPRSKTPS 480
SPCPASKVVR PLRTFLHTVQ RNQMLMTPTS APRSVMKSFI KRNTPLRMDP KCSFVEKERQ 540
RLENLRRKEE AEQLRRQKVE EDKRRRLEEV KLKREERLRK VLQARERVEQ MKEEKKKQIE 600
QKFAQIDEKT EKAKEERLAE EKAKKKAAAK KMEEVEARRK QEEEARRLRW LQQEEEERRH 660
QELLQKKKEE EQERLRKAAE AKRLAEQREQ ERREQERREQ ERREQERREQ ERREQERQLA 720
EQERRREQER LQAERELQER EKALRLQKEQ LQRELEEKKK KEEQQRLAER QLQEEQEKKA 780
KEAAGASKAL NVTVDVQSPA CTSYQMTPQG HRAPPKINPD NYGMDLNSDD STDDEAHPRK 840
PIPTWARGTP LSQAIIHQYY HPPNLLELFG TILPLDLEDI FKKSKPRYHK RTSSAVWNSP 900
PLQGARVPSS LAYSLKKH 918 
Gene Ontology
 GO:0000801; C:central element; IEA:Compara.
 GO:0005721; C:centromeric heterochromatin; IDA:UniProtKB.
 GO:0010369; C:chromocenter; IEA:Compara.
 GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0000776; C:kinetochore; IDA:UniProtKB.
 GO:0000800; C:lateral element; IEA:Compara.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0030496; C:midbody; IEA:Compara.
 GO:0005819; C:spindle; IDA:UniProtKB.
 GO:0007059; P:chromosome segregation; IMP:UniProtKB.
 GO:0000910; P:cytokinesis; IMP:UniProtKB.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome. 
Interpro
 IPR022006; INCENP_N.
 IPR005635; Inner_centromere_prot_ARK-bd. 
Pfam
 PF03941; INCENP_ARK-bind
 PF12178; INCENP_N 
SMART
  
PROSITE
  
PRINTS