| Tag | Content |
|---|
| CPLM ID | CPLM-010305 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | N-ethylmaleimide reductase |
Protein Synonyms/Alias | N-ethylmaleimide reducing enzyme |
Gene Name | nemA |
Gene Synonyms/Alias | ydhN; b1650; JW1642 |
Created Date | July 27, 2013 |
Organism | Escherichia coli (strain K12) |
NCBI Taxa ID | 83333 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 85 | PEQIAAWKKITAGVH | acetylation | [1] | | 263 | YLIEQLGKRGIAYLH | acetylation | [1] | | 315 | KAETLIGKGLIDAVA | acetylation | [1] |
|
Reference | [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli. Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C. Mol Cell. 2013 Jul 25;51(2):265-72. [ PMID: 23830618] |
Functional Description | Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide. |
Sequence Annotation | REGION 182 185 Substrate-binding (By similarity).
ACT_SITE 187 187 Proton donor (By similarity).
BINDING 101 101 FMN (By similarity).
BINDING 234 234 FMN (By similarity).
BINDING 325 325 FMN (By similarity). |
Keyword | Complete proteome; Flavoprotein; FMN; NADP; Oxidoreductase; Reference proteome. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 365 AA |
Protein Sequence | MSSEKLYSPL KVGAITAANR IFMAPLTRLR SIEPGDIPTP LMAEYYRQRA SAGLIISEAT 60
QISAQAKGYA GAPGIHSPEQ IAAWKKITAG VHAENGHMAV QLWHTGRISH ASLQPGGQAP 120
VAPSALSAGT RTSLRDENGQ AIRVETSMPR ALELEEIPGI VNDFRQAIAN AREAGFDLVE 180
LHSAHGYLLH QFLSPSSNHR TDQYGGSVEN RARLVLEVVD AGIEEWGADR IGIRVSPIGT 240
FQNTDNGPNE EADALYLIEQ LGKRGIAYLH MSEPDWAGGE PYTDAFREKV RARFHGPIIG 300
AGAYTVEKAE TLIGKGLIDA VAFGRDWIAN PDLVARLQRK AELNPQRAES FYGGGAEGYT 360
DYPTL 365 |
Gene Ontology | GO:0010181; F:FMN binding; IEA:InterPro. GO:0008748; F:N-ethylmaleimide reductase activity; IDA:EcoCyc. GO:0046857; F:oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor; IDA:EcoCyc. GO:0006805; P:xenobiotic metabolic process; IDA:EcoCyc. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |