CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023186
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 E3 ubiquitin-protein ligase ZNRF3 
Protein Synonyms/Alias
 RING finger protein 203; Zinc/RING finger protein 3 
Gene Name
 ZNRF3 
Gene Synonyms/Alias
 KIAA1133; RNF203 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
261LAVQALEKMETRKFNubiquitination[1, 2]
341RHNIIEQKGNPSAVCubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt receptor complex components Frizzled and LRP6. Acts on both canonical and non- canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone. 
Sequence Annotation
 ZN_FING 293 334 RING-type; atypical.  
Keyword
 Alternative splicing; Cell membrane; Complete proteome; Ligase; Membrane; Metal-binding; Reference proteome; Signal; Transmembrane; Transmembrane helix; Ubl conjugation pathway; Wnt signaling pathway; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 936 AA 
Protein Sequence
MRPRSGGRPG ATGRRRRRLR RRPRGLRCSR LPPPPPLPLL LGLLLAAAGP GAARAKETAF 60
VEVVLFESSP SGDYTTYTTG LTGRFSRAGA TLSAEGEIVQ MHPLGLCNNN DEEDLYEYGW 120
VGVVKLEQPE LDPKPCLTVL GKAKRAVQRG ATAVIFDVSE NPEAIDQLNQ GSEDPLKRPV 180
VYVKGADAIK LMNIVNKQKV ARARIQHRPP RQPTEYFDMG IFLAFFVVVS LVCLILLVKI 240
KLKQRRSQNS MNRLAVQALE KMETRKFNSK SKGRREGSCG ALDTLSSSST SDCAICLEKY 300
IDGEELRVIP CTHRFHRKCV DPWLLQHHTC PHCRHNIIEQ KGNPSAVCVE TSNLSRGRQQ 360
RVTLPVHYPG RVHRTNAIPA YPTRTSMDSH GNPVTLLTMD RHGEQSLYSP QTPAYIRSYP 420
PLHLDHSLAA HRCGLEHRAY SPAHPFRRPK LSGRSFSKAA CFSQYETMYQ HYYFQGLSYP 480
EQEGQSPPSL APRGPARAFP PSGSGSLLFP TVVHVAPPSH LESGSTSSFS CYHGHRSVCS 540
GYLADCPGSD SSSSSSSGQC HCSSSDSVVD CTEVSNQGVY GSCSTFRSSL SSDYDPFIYR 600
SRSPCRASEA GGSGSSGRGP ALCFEGSPPP EELPAVHSHG AGRGEPWPGP ASPSGDQVST 660
CSLEMNYSSN SSLEHRGPNS STSEVGLEAS PGAAPDLRRT WKGGHELPSC ACCCEPQPSP 720
AGPSAGAAGS STLFLGPHLY EGSGPAGGEP QSGSSQGLYG LHPDHLPRTD GVKYEGLPCC 780
FYEEKQVARG GGGGSGCYTE DYSVSVQYTL TEEPPPGCYP GARDLSQRIP IIPEDVDCDL 840
GLPSDCQGTH SLGSWGGTRG PDTPRPHRGL GATREEERAL CCQARALLRP GCPPEEAGAV 900
RANFPSALQD TQESSTTATE AAGPRSHSAD SSSPGA 936 
Gene Ontology
 GO:0005887; C:integral to plasma membrane; IDA:UniProtKB.
 GO:0004842; F:ubiquitin-protein ligase activity; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0060070; P:canonical Wnt receptor signaling pathway; IEA:Compara.
 GO:0090090; P:negative regulation of canonical Wnt receptor signaling pathway; IMP:UniProtKB.
 GO:2000051; P:negative regulation of non-canonical Wnt receptor signaling pathway; IMP:UniProtKB.
 GO:0072089; P:stem cell proliferation; ISS:UniProtKB.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IMP:UniProtKB.
 GO:0038018; P:Wnt receptor catabolic process; IMP:UniProtKB.
 GO:0060071; P:Wnt receptor signaling pathway, planar cell polarity pathway; IEA:Compara. 
Interpro
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD. 
Pfam
 PF13639; zf-RING_2 
SMART
 SM00184; RING 
PROSITE
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 
PRINTS