UniProt Accession

 NADR_ECOLI; P27278; P76819; Q2M5S7 

Genbank Protein ID

 U14003; U00096; AP009048; X63155 

Genbank Nucleotide ID

 AAA97286.1; AAC77343.2; BAE78379.1 

Protein Name

 Trifunctional NAD biosynthesis/regulator protein NadR 

Protein Synonyms/Alias

 Transcriptional regulator NadR; Nicotinamide mononucleotide adenylyltransferase; NMN adenylyltransferase; NMN-AT; NMNAT; Nicotinamide ribonucleotide adenylyltransferase; Nicotinamide-nucleotide adenylyltransferase; Ribosylnicotinamide kinase; RNK; Nicotinamide riboside kinase; NRK; NmR-K 

Gene Name

 nadR 

Gene Synonyms/Alias

 nadI; b4390; JW5800 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
41	QLLNAKIKSPSAQKL	acetylation	[1]
47	IKSPSAQKLEALHRF	acetylation	[1]
72	TIGVVFGKFYPLHTG	acetylation	[1]
132	RWLLQTFKYQKNIRI	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 This enzyme has three activities: DNA binding, nicotinamide mononucleotide (NMN) adenylyltransferase and ribosylnicotinamide (RN) kinase. The DNA-binding domain binds to the nadB operator sequence in an NAD- and ATP-dependent manner. As NAD levels increase within the cell, the affinity of NadR for the nadB operator regions of nadA, nadB, and pncB increases, repressing the transcription of these genes. The RN kinase activity catalyzes the phosphorylation of RN to form nicotinamide ribonucleotide. The NMN adenylyltransferase activity catalyzes the transfer of the AMP moiety of ATP to nicotinamide ribonucleotide to form NAD(+). The NMN adenylyltransferase domain also functions as the NAD and ATP sensor. 

Sequence Annotation

 DOMAIN 7 62 HTH cro/C1-type.
 DNA_BIND 18 37 H-T-H motif (Probable).
 NP_BIND 70 73 NAD 1 (By similarity).
 NP_BIND 144 157 NAD 1 (By similarity).
 NP_BIND 177 179 NAD 1 (By similarity).
 NP_BIND 204 206 NAD 1 (By similarity).
 NP_BIND 259 261 NAD 2 (By similarity).
 NP_BIND 294 297 NAD 2 (By similarity).
 REGION 63 229 Nicotinamide mononucleotide
 REGION 230 410 Ribosylnicotinamide kinase.
 BINDING 77 77 NAD 1 (By similarity).
 BINDING 104 104 NAD 1 (By similarity).  

Keyword

 ATP-binding; Cell membrane; Complete proteome; Cytoplasm; DNA-binding; Kinase; Membrane; Multifunctional enzyme; NAD; Nucleotide-binding; Pyridine nucleotide biosynthesis; Reference proteome; Repressor; Transcription; Transcription regulation; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 410 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0000986; F:bacterial-type RNA polymerase core promoter proximal region sequence-specific DNA binding; IDA:EcoCyc.
 GO:0000287; F:magnesium ion binding; IDA:EcoCyc.
 GO:0000309; F:nicotinamide-nucleotide adenylyltransferase activity; IDA:EcoCyc.
 GO:0050262; F:ribosylnicotinamide kinase activity; IEA:EC.
 GO:0009435; P:NAD biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IEA:InterPro.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006810; P:transport; IEA:InterPro. 

Interpro

 IPR016429; Bifunc_transcrip_reg_NadR.
 IPR004821; Cyt_trans-like.
 IPR001387; HTH.
 IPR010982; Lambda_DNA-bd_dom.
 IPR006417; NadR_NMN_Atrans.
 IPR027417; P-loop_NTPase.
 IPR014729; Rossmann-like_a/b/a_fold. 

Pfam

 PF01381; HTH_3 

SMART

 SM00530; HTH_XRE 

PROSITE

 PS50943; HTH_CROC1 

PRINTS