UniProt Accession

 CURA_ECOLI; P76113; P78255 

Genbank Protein ID

 U00096; AP009048 

Genbank Nucleotide ID

 AAC74531.4; BAA15081.1 

Protein Name

 NADPH-dependent curcumin reductase 

Protein Synonyms/Alias

 NADPH-dependent curcumin/dihydrocurcumin reductase 

Gene Name

 curA 

Gene Synonyms/Alias

 yncB; b1449; JW5907 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
186	GVAGGAEKCRHATEV	acetylation	[1, 2]
217	QLAKACPKGIDIYYE	acetylation	[2]
304	REMGQWVKEDKIHYR	acetylation	[2]
307	GQWVKEDKIHYREEI	acetylation	[2]
330	QTFIGLLKGKNFGKV	acetylation	[2]
336	LKGKNFGKVVIRVAG	acetylation	[2]

Reference

 [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Catalyzes the metal-independent reduction of curcumin to dihydrocurcumin (DHC) as an intermediate product, followed by further reduction to tetrahydrocurcumin (THC) as an end product. It also acts on 3-octene-2-one, 3-hepten-2-one, resveratrol, and trans-2-octenal. 

Sequence Annotation

 NP_BIND 160 163 NADP (By similarity).
 NP_BIND 182 186 NADP (By similarity).
 NP_BIND 247 253 NADP (By similarity).
 NP_BIND 282 284 NADP (By similarity).
 BINDING 51 51 NADP (By similarity).
 BINDING 186 186 NADP (By similarity).
 BINDING 225 225 NADP (By similarity).
 BINDING 333 333 NADP (By similarity).  

Keyword

 Complete proteome; NADP; Oxidoreductase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 345 AA 

Protein Sequence

Gene Ontology

 GO:0016628; F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor; IEA:EC.
 GO:0008270; F:zinc ion binding; IEA:InterPro. 

Interpro

 IPR013149; ADH_C.
 IPR002085; ADH_SF_Zn-type.
 IPR011032; GroES-like.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF00107; ADH_zinc_N 

SMART

  

PROSITE

  

PRINTS