UniProt Accession

 HMGCL_RAT; P97519 

Genbank Protein ID

 Y10054; BC061797 

Genbank Nucleotide ID

 CAA71148.1; AAH61797.1 

Protein Name

 Hydroxymethylglutaryl-CoA lyase, mitochondrial 

Protein Synonyms/Alias

 HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase 

Gene Name

 Hmgcl 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
48	RDGLQNEKSIVPTPV	acetylation	[1]
58	VPTPVKIKLIDMLSE	acetylation	[1]
111	PVLTPNMKGFEEAVA	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Key enzyme in ketogenesis (ketone body formation). Terminal step in leucine catabolism. Ketone bodies (beta- hydroxybutyrate, acetoacetate and acetone) are essential as an alternative source of energy to glucose, as lipid precursors and as regulators of metabolism (By similarity). 

Sequence Annotation

 MOTIF 323 325 Microbody targeting signal (Potential).
 ACT_SITE 266 266 By similarity.
 METAL 42 42 Divalent metal cation (By similarity).
 METAL 233 233 Divalent metal cation (By similarity).
 METAL 235 235 Divalent metal cation (By similarity).
 METAL 275 275 Divalent metal cation (By similarity).
 BINDING 41 41 Substrate (By similarity).
 MOD_RES 48 48 N6-acetyllysine (By similarity).
 DISULFID 323 323 Interchain (By similarity).  

Keyword

 Acetylation; Complete proteome; Disulfide bond; Lyase; Metal-binding; Mitochondrion; Peroxisome; Reference proteome; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 325 AA 

Protein Sequence

Gene Ontology

 GO:0005743; C:mitochondrial inner membrane; IEA:Compara.
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:RGD.
 GO:0005777; C:peroxisome; IEA:UniProtKB-SubCell.
 GO:0031406; F:carboxylic acid binding; IPI:RGD.
 GO:0000062; F:fatty-acyl-CoA binding; IPI:RGD.
 GO:0004419; F:hydroxymethylglutaryl-CoA lyase activity; ISS:UniProtKB.
 GO:0000287; F:magnesium ion binding; IEA:Compara.
 GO:0030145; F:manganese ion binding; IEA:Compara.
 GO:0046872; F:metal ion binding; ISS:UniProtKB.
 GO:0006637; P:acyl-CoA metabolic process; IDA:RGD.
 GO:0009790; P:embryo development; IEA:Compara.
 GO:0046951; P:ketone body biosynthetic process; IDA:RGD.
 GO:0001889; P:liver development; IEP:RGD.
 GO:0007005; P:mitochondrion organization; IEA:Compara.
 GO:0051262; P:protein tetramerization; IEA:Compara.
 GO:0070542; P:response to fatty acid; IEP:RGD.
 GO:0007584; P:response to nutrient; IEP:RGD.
 GO:0042594; P:response to starvation; IEP:RGD. 

Interpro

 IPR013785; Aldolase_TIM.
 IPR027167; HMG-CoA_lyase.
 IPR000138; HMG_CoA_lyase_AS.
 IPR000891; PYR_CT. 

Pfam

 PF00682; HMGL-like 

SMART

  

PROSITE

 PS01062; HMG_COA_LYASE
 PS50991; PYR_CT 

PRINTS