CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000495
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Pyruvate, phosphate dikinase 1, chloroplastic 
Protein Synonyms/Alias
 Pyruvate, orthophosphate dikinase 1 
Gene Name
 PPDK 
Gene Synonyms/Alias
 At4g15530; dl3805c; FCAALL.325 
Created Date
 July 27, 2013 
Organism
 Arabidopsis thaliana (Mouse-ear cress) 
NCBI Taxa ID
 3702 
Lysine Modification
Position
Peptide
Type
References
105KGRSEGNKGMKSLLGubiquitination[1]
Reference
 [1] Multidimensional protein identification technology (MudPIT) analysis of ubiquitinated proteins in plants.
 Maor R, Jones A, Nühse TS, Studholme DJ, Peck SC, Shirasu K.
 Mol Cell Proteomics. 2007 Apr;6(4):601-10. [PMID: 17272265] 
Functional Description
 Formation of phosphoenolpyruvate. May be involved in regulating the flux of carbon into starch and fatty acids of seeds and in the remobilization of nitrogen reserves in senescing leaves. 
Sequence Annotation
 ACT_SITE 545 545 Tele-phosphohistidine intermediate (By
 ACT_SITE 922 922 Proton donor (By similarity).
 METAL 836 836 Magnesium (By similarity).
 METAL 860 860 Magnesium (By similarity).
 BINDING 651 651 Substrate (By similarity).
 BINDING 707 707 Substrate (By similarity).
 BINDING 836 836 Substrate (By similarity).
 BINDING 857 857 Substrate; via carbonyl oxygen (By
 BINDING 858 858 Substrate; via amide nitrogen (By
 BINDING 859 859 Substrate (By similarity).
 BINDING 860 860 Substrate; via amide nitrogen (By
 MOD_RES 543 543 Phosphothreonine; by PDRP1 (By
 MOD_RES 544 544 Phosphoserine.
 CROSSLNK 105 105 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Alternative promoter usage; Alternative splicing; ATP-binding; Chloroplast; Complete proteome; Cytoplasm; Isopeptide bond; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein; Photosynthesis; Plastid; Pyruvate; Reference proteome; Transferase; Transit peptide; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 963 AA 
Protein Sequence
MLYIRKKMTS MIVKTTPELF KGNGVFRTDH LGENRMVSRS NRLGDGSNRF PRTGTIHCQR 60
LSIAKTGLHR ETKARAILSP VSDPAASIAQ KRVFTFGKGR SEGNKGMKSL LGGKGANLAE 120
MASIGLSVPP GLTISTEACQ QYQIAGKKLP EGLWEEILEG LSFIERDIGA SLADPSKPLL 180
LSVRSGAAIS MPGMMDTVLN LGLNDQVVVG LAAKSGERFA YDSFRRFLDM FGDVVMGIPH 240
AKFEEKLERM KERKGVKNDT DLSAADLKEL VEQYKSVYLE AKGQEFPSDP KKQLELAIEA 300
VFDSWDSPRA NKYRSINQIT GLKGTAVNIQ CMVFGNMGDT SGTGVLFTRN PSTGEKKLYG 360
EFLVNAQGED VVAGIRTPED LDTMKRFMPE AYAELVENCN ILERHYKDMM DIEFTVQEER 420
LWMLQCRAGK RTGKGAVKIA VDMVGEGLVE KSSAIKMVEP QHLDQLLHPQ FHDPSGYREK 480
VVAKGLPASP GAAVGQVVFT AEEAEAWHSQ GKTVILVRTE TSPDDVGGMH AAEGILTARG 540
GMTSHAAVVA RGWGKCCIAG CSEIRVDENH KVLLIGDLTI NEGEWISMNG STGEVILGKQ 600
ALAPPALSPD LETFMSWADA IRRLKVMANA DTPEDAIAAR KNGAQGIGLC RTEHMFFGAD 660
RIKAVRKMIM AVTTEQRKAS LDILLPYQRS DFEGIFRAMD GLPVTIRLLD PPLHEFLPEG 720
DLDNIVHELA EETGVKEDEV LSRIEKLSEV NPMLGFRGCR LGISYPELTE MQARAIFEAA 780
ASMQDQGVTV IPEIMVPLVG TPQELGHQVD VIRKVAKKVF AEKGHTVSYK VGTMIEIPRA 840
ALIADEIAKE AEFFSFGTND LTQMTFGYSR DDVGKFLPIY LAKGILQHDP FEVLDQQGVG 900
QLIKMATEKG RAARPSLKVG ICGEHGGDPS SVGFFAEAGL DYVSCSPFRV PIARLAAAQV 960
VVA 963 
Gene Ontology
 GO:0009570; C:chloroplast stroma; IDA:TAIR.
 GO:0005829; C:cytosol; IDA:TAIR.
 GO:0005634; C:nucleus; IDA:TAIR.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0050242; F:pyruvate, phosphate dikinase activity; IEA:EC.
 GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
 GO:0006090; P:pyruvate metabolic process; IEA:InterPro. 
Interpro
 IPR013815; ATP_grasp_subdomain_1.
 IPR013816; ATP_grasp_subdomain_2.
 IPR008279; PEP-util_enz_mobile_dom.
 IPR018274; PEP_util_AS.
 IPR000121; PEP_util_C.
 IPR023151; PEP_util_CS.
 IPR002192; PPDK_PEP-bd.
 IPR010121; Pyruvate_phosphate_dikinase.
 IPR015813; Pyrv/PenolPyrv_Kinase-like_dom. 
Pfam
 PF00391; PEP-utilizers
 PF02896; PEP-utilizers_C
 PF01326; PPDK_N 
SMART
  
PROSITE
 PS00742; PEP_ENZYMES_2
 PS00370; PEP_ENZYMES_PHOS_SITE 
PRINTS