CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014355
UniProt Accession
Genbank Protein ID
 D49920 
Genbank Nucleotide ID
Protein Name
 NADPH:adrenodoxin oxidoreductase, mitochondrial 
Protein Synonyms/Alias
 AR; Adrenodoxin reductase; Ferredoxin--NADP(+) reductase; Ferredoxin reductase 
Gene Name
 Fdxr 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
242LQVAFTIKELREMIQacetylation[1, 2]
290LLRTATEKPGVEEAAacetylation[1]
379PSVPFDPKLGVIPNTacetylation[1, 2, 3]
463VSFSDWEKLDAEEVSacetylation[1]
Reference
 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 
Functional Description
 Serves as the first electron transfer protein in all the mitochondrial P450 systems. Including cholesterol side chain cleavage in all steroidogenic tissues, steroid 11-beta hydroxylation in the adrenal cortex, 25-OH-vitamin D3-24 hydroxylation in the kidney, and sterol C-27 hydroxylation in the liver. 
Sequence Annotation
 NP_BIND 187 190 NADP (By similarity).
 NP_BIND 231 232 NADP (By similarity).
 NP_BIND 408 410 FAD (By similarity).
 BINDING 51 51 FAD; via amide nitrogen (By similarity).
 BINDING 72 72 FAD (By similarity).
 BINDING 80 80 FAD; via amide nitrogen (By similarity).
 BINDING 116 116 FAD; via amide nitrogen and carbonyl
 BINDING 243 243 NADP (By similarity).
 BINDING 401 401 FAD; via amide nitrogen (By similarity).
 BINDING 408 408 NADP; via amide nitrogen (By similarity).  
Keyword
 Cholesterol metabolism; Complete proteome; Electron transport; FAD; Flavoprotein; Lipid metabolism; Mitochondrion; NADP; Oxidoreductase; Reference proteome; Steroid metabolism; Sterol metabolism; Transit peptide; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 494 AA 
Protein Sequence
MAPRCWHWWR WSAWSGLRPS PSRSTPTPGF CQKFSTQEKT PQICVVGSGP AGFYTAQHLL 60
KHHTHAHVDI YEKQLVPFGL VRFGVAPDHP EVKNVINTFT QTARSDRCAF QGNVVVGRDV 120
SVPELREAYH AVVLSYGAED HQPLGIPGEE LPGVVSARAF VGWYNGLPEN QELAPDLSCD 180
TAVILGQGNV ALDVARILLT PPEHLEKTDI TEAALGALRQ SRVKTVWIVG RRGPLQVAFT 240
IKELREMIQL PGTRPILDPS DFLGLQDRIK DVPRPRRRLT ELLLRTATEK PGVEEAARQA 300
LASRAWGLRF FRSPQQVLPT PDGQRVAGIR LAVTSLEGVG ESTRAVPTGD VEDLPCGLLL 360
SSVGYKSRPI DPSVPFDPKL GVIPNTEGRV VNVPGLYCSG WVKRGPTGVI TTTMTDSFLT 420
SQALLEDLKA GLLPSGPRPG YVAIQALLSN RGVRPVSFSD WEKLDAEEVS RGQGTGKPRE 480
KLVDRREMLR LLGH 494 
Gene Ontology
 GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0070402; F:NADPH binding; IEA:Compara.
 GO:0015039; F:NADPH-adrenodoxin reductase activity; IEA:Compara.
 GO:0008203; P:cholesterol metabolic process; IEA:UniProtKB-UniPathway.
 GO:0022900; P:electron transport chain; IEA:UniProtKB-KW.
 GO:0070995; P:NADPH oxidation; IEA:Compara. 
Interpro
 IPR021163; Adrenodoxin_Rdtase.
 IPR016040; NAD(P)-bd_dom. 
Pfam
  
SMART
  
PROSITE
  
PRINTS