CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012535
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Lethal(2) giant larvae protein homolog 1 
Protein Synonyms/Alias
 LLGL; DLG4; Hugl-1; Human homolog to the D-lgl gene protein 
Gene Name
 LLGL1 
Gene Synonyms/Alias
 DLG4; HUGL; HUGL1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
17GADPQREKLKQELFAubiquitination[1, 2]
19DPQREKLKQELFAFNubiquitination[1, 3, 4, 5]
27QELFAFNKTVEHGFPubiquitination[1, 3, 5]
59GTRSGAVKIYGAPGVubiquitination[1, 2]
293FPCKAINKILWRNCEubiquitination[1, 3, 5]
502DDWPPFRKVGCFDPYubiquitination[1, 2]
519DPRLGVQKVALCKYTubiquitination[1, 2]
569DREGFTWKGHERLSPubiquitination[1, 2]
684RAANASSKLQEANAQubiquitination[1, 6]
780AVEAVLGKEVQLMHRubiquitination[1]
848VSAKTKFKLTAHEGCubiquitination[1]
906VHYSCIRKEDISGIAubiquitination[1, 2]
1046GSSEESEKNLRNLAEubiquitination[4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
 Cortical cytoskeleton protein found in a complex involved in maintaining cell polarity and epithelial integrity. Involved in the regulation of mitotic spindle orientation, proliferation, differentiation and tissue organization of neuroepithelial cells. Involved in axonogenesis through RAB10 activation thereby regulating vesicular membrane trafficking toward the axonal plasma membrane. 
Sequence Annotation
 REPEAT 38 71 WD 1.
 REPEAT 78 119 WD 2.
 REPEAT 139 176 WD 3.
 REPEAT 200 234 WD 4.
 REPEAT 240 272 WD 5.
 REPEAT 290 332 WD 6.
 REPEAT 340 374 WD 7.
 REPEAT 396 474 WD 8.
 REPEAT 518 593 WD 9.
 REPEAT 602 663 WD 10.
 REPEAT 723 783 WD 11.
 REPEAT 792 844 WD 12.
 REPEAT 849 902 WD 13.
 REPEAT 916 939 WD 14.
 MOD_RES 663 663 Phosphoserine.
 MOD_RES 958 958 Phosphothreonine (By similarity).
 MOD_RES 967 967 Phosphoserine (By similarity).  
Keyword
 Cell projection; Complete proteome; Cytoplasm; Cytoskeleton; Endosome; Exocytosis; Golgi apparatus; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1064 AA 
Protein Sequence
MMKFRFRRQG ADPQREKLKQ ELFAFNKTVE HGFPNQPSAL AFDPELRIMA IGTRSGAVKI 60
YGAPGVEFTG LHRDAATVTQ MHFLTGQGRL LSLLDDSSLH LWEIVHHNGC AHLEEALSFQ 120
LPSRPGFDGA SAPLSLTRVT VVLLVAASDI AALGTEGSSV FFLDVTTLTL LEGQTLAPGE 180
VLRSVPDDYR CGKALGPVES LQGHLRDPTK ILIGYSRGLL VIWNQASQCV DHIFLGNQQL 240
ESLCWGRDSS TVVSSHSDGS YAVWSVDAGS FPTLQPTVAT TPYGPFPCKA INKILWRNCE 300
SGGHFIIFSG GMPRASYGDR HCVSVLRAET LVTLDFTSRI IDFFTVHSTR PEDEFDDPQA 360
LAVLLEEELV VLDLQTPGWP AVPAPYLAPL HSSAITCSAH VASVPAKLWA RIVSAGEQQS 420
PQPVSSALSW PITGGRNLAQ EPSQRGLLLT GHEDGTVRFW DASGVALRPL YKLSTAGLFQ 480
TDCEHADSLA QAAEDDWPPF RKVGCFDPYS DDPRLGVQKV ALCKYTAQMV VAGTAGQVLV 540
LELSDVPVEQ AVSVAIIDLL QDREGFTWKG HERLSPRTGP LPWPAGFQPR VLVQCLPPAA 600
VTAVTLHTEW SLVAFGTSHG FGLFDYQRKS PVLARCTLHP NDSLAMEGPL SRVKSLKKSL 660
RQSFRRIRKS RVSGKKRAAN ASSKLQEANA QLAEQACPHD VEMTPVQRRI EPRSADDSLS 720
GVVRCLYFAD TFLRDGAHHG PTMWAGTNSG SVFAYALEVP AAAVGGEKRP EQAVEAVLGK 780
EVQLMHRAPV VAIAVLDGRG RPLPEPYEAS RDLAQAPDMQ GGHAVLIASE EQFKVFTLPK 840
VSAKTKFKLT AHEGCRVRKV ALATFASVAC EDYAETCLAC LTNLGDVHVF SVPGLRPQVH 900
YSCIRKEDIS GIASCVFTRH GQGFYLISPS EFERFSLSAR NITEPLCSLD INWPRDATQA 960
SYRIRESPKL SQANGTPSIL LAPQSLDGSP DPAHSMGPDT PEPPEAALSP MSIDSATSAD 1020
TTLDTTGDVT VEDVKDFLGS SEESEKNLRN LAEDEAHACA ILIK 1064 
Gene Ontology
 GO:0030424; C:axon; IEA:UniProtKB-SubCell.
 GO:0030864; C:cortical actin cytoskeleton; IDA:UniProtKB.
 GO:0031901; C:early endosome membrane; ISS:UniProtKB.
 GO:0000137; C:Golgi cis cisterna; ISS:UniProtKB.
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0035748; C:myelin sheath abaxonal region; IEA:Compara.
 GO:0032588; C:trans-Golgi network membrane; ISS:UniProtKB.
 GO:0019901; F:protein kinase binding; IDA:UniProtKB.
 GO:0005097; F:Rab GTPase activator activity; ISS:UniProtKB.
 GO:0005198; F:structural molecule activity; TAS:ProtInc.
 GO:0007409; P:axonogenesis; ISS:UniProtKB.
 GO:0030866; P:cortical actin cytoskeleton organization; IDA:UniProtKB.
 GO:0006887; P:exocytosis; IEA:UniProtKB-KW.
 GO:0006893; P:Golgi to plasma membrane transport; ISS:UniProtKB.
 GO:0035090; P:maintenance of apical/basal cell polarity; IEA:Compara.
 GO:0006461; P:protein complex assembly; IDA:UniProtKB. 
Interpro
 IPR000664; Lethal2_giant.
 IPR013577; LLGL2.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF08366; LLGL 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00962; LETHAL2GIANT.