UniProt Accession

 G3P1_ECOLI; P0A9B2; P06977 

Genbank Protein ID

 X02662; U00096; AP009048; M66870; M66871; M66872; M66873; M66874; M66875; M66876; M66877; M66878; M66879; M66880; M66881; M66882; U07750; U07751; U07752; U07754; U07765; U07768; U07769; U07770; U07771; U07772; U07773 

Genbank Nucleotide ID

 CAA26498.1; AAC74849.1; BAA15576.1; AAA23838.1; AAA23839.1; AAA02930.1; AAA23840.1; AAA23841.1; AAA23842.1; AAA23843.1; AAA23844.1; AAA23845.1; AAA23846.1; AAA23847.1; AAA23848.1; AAA23849.1; AAC43271.1; AAC43272.1; AAC43273.1; AAC43274.1; AAC43284.1; AAC43285.1; AAC43286.1; AAC43287.1; AAC43288.1; AAC43289.1; AAC43290.1 

Protein Name

 Glyceraldehyde-3-phosphate dehydrogenase A 

Protein Synonyms/Alias

 GAPDH-A 

Gene Name

 gapA 

Gene Synonyms/Alias

 b1779; JW1768 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
4	****MTIKVGINGFG	acetylation	[1]
46	DYMAYMLKYDSTHGR	acetylation	[1, 2]
61	FDGTVEVKDGHLIVN	acetylation	[1, 2]
70	GHLIVNGKKIRVTAE	acetylation	[2]
71	HLIVNGKKIRVTAER	acetylation	[2]
108	LTDETARKHITAGAK	acetylation	[1, 2]
115	KHITAGAKKVVMTGP	acetylation	[1, 2]
116	HITAGAKKVVMTGPS	acetylation	[1, 2]
124	VVMTGPSKDNTPMFV	acetylation	[1, 2]
132	DNTPMFVKGANFDKY	acetylation	[1, 2, 3, 4]
138	VKGANFDKYAGQDIV	acetylation	[1, 2, 3]
160	NCLAPLAKVINDNFG	acetylation	[1]
184	HATTATQKTVDGPSH	acetylation	[1, 2, 4]
192	TVDGPSHKDWRGGRG	acetylation	[1, 2, 3, 4]
213	PSSTGAAKAVGKVLP	acetylation	[1, 2]
217	GAAKAVGKVLPELNG	acetylation	[1, 2]
225	VLPELNGKLTGMAFR	acetylation	[1, 2]
249	DLTVRLEKAATYEQI	acetylation	[1, 2, 3]
249	DLTVRLEKAATYEQI	pupylation	[5]
257	AATYEQIKAAVKAAA	acetylation	[1, 2]
261	EQIKAAVKAAAEGEM	acetylation	[1, 2]
321	NETGYSNKVLDLIAH	acetylation	[1, 2]
331	DLIAHISK*******	acetylation	[1]

Reference

 [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [3] Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.
 Zhang J, Sprung R, Pei J, Tan X, Kim S, Zhu H, Liu CF, Grishin NV, Zhao Y.
 Mol Cell Proteomics. 2009 Feb;8(2):215-25. [PMID: 18723842]
 [4] The diversity of lysine-acetylated proteins in Escherichia coli.
 Yu BJ, Kim JA, Moon JH, Ryu SE, Pan JG.
 J Microbiol Biotechnol. 2008 Sep;18(9):1529-36. [PMID: 18852508]
 [5] Reconstitution of the Mycobacterium tuberculosis pupylation pathway in Escherichia coli.
 Cerda-Maira FA, McAllister F, Bode NJ, Burns KE, Gygi SP, Darwin KH.
 EMBO Rep. 2011 Jul 8;12(8):863-70. [PMID: 21738222] 

Functional Description

  

Sequence Annotation

 NP_BIND 12 13 NAD (By similarity).
 REGION 149 151 Glyceraldehyde 3-phosphate binding.
 REGION 209 210 Glyceraldehyde 3-phosphate binding.
 ACT_SITE 150 150 Nucleophile.
 BINDING 34 34 NAD.
 BINDING 78 78 NAD; via carbonyl oxygen.
 BINDING 180 180 Glyceraldehyde 3-phosphate.
 BINDING 232 232 Glyceraldehyde 3-phosphate.
 BINDING 314 314 NAD.
 MOD_RES 115 115 N6-succinyllysine.
 MOD_RES 124 124 N6-succinyllysine.
 MOD_RES 132 132 N6-acetyllysine; alternate.
 MOD_RES 132 132 N6-succinyllysine; alternate.
 MOD_RES 138 138 N6-acetyllysine.
 MOD_RES 192 192 N6-acetyllysine; alternate.
 MOD_RES 192 192 N6-succinyllysine; alternate.
 MOD_RES 213 213 N6-succinyllysine.
 MOD_RES 217 217 N6-succinyllysine.
 MOD_RES 225 225 N6-succinyllysine.
 MOD_RES 249 249 N6-acetyllysine.
 MOD_RES 249 249 N6-succinyllysine.
 MOD_RES 257 257 N6-succinyllysine.
 MOD_RES 261 261 N6-succinyllysine.
 MOD_RES 331 331 N6-malonyllysine; alternate.
 MOD_RES 331 331 N6-succinyllysine; alternate.  

Keyword

 3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; NAD; Oxidoreductase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 331 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0016020; C:membrane; IDA:UniProtKB.
 GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; IEA:EC.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0050661; F:NADP binding; IEA:InterPro.
 GO:0006096; P:glycolysis; IEA:UniProtKB-UniPathway. 

Interpro

 IPR020831; GlycerAld/Erythrose_P_DH.
 IPR020830; GlycerAld_3-P_DH_AS.
 IPR020829; GlycerAld_3-P_DH_cat.
 IPR020828; GlycerAld_3-P_DH_NAD(P)-bd.
 IPR006424; Glyceraldehyde-3-P_DH_1.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF02800; Gp_dh_C
 PF00044; Gp_dh_N 

SMART

 SM00846; Gp_dh_N 

PROSITE

 PS00071; GAPDH 

PRINTS

 PR00078; G3PDHDRGNASE.