CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008202
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Translation initiation factor eIF-2B subunit beta 
Protein Synonyms/Alias
 S20I15; S20III15; eIF-2B GDP-GTP exchange factor subunit beta 
Gene Name
 EIF2B2 
Gene Synonyms/Alias
 EIF2BB 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
7*MPGSAAKGSELSERubiquitination[1]
23ESFVETLKRGGGPRSubiquitination[1]
87NMVRRVLKIIREEYGubiquitination[1]
179RTVEAFLKEAARKRKubiquitination[1, 2]
231AVMSRVNKVIIGTKTubiquitination[1]
237NKVIIGTKTILANGAubiquitination[1, 3, 4, 5]
287NEEDSFHKFVAPEEVubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. 
Sequence Annotation
  
Keyword
 Complete proteome; Disease mutation; Initiation factor; Leukodystrophy; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 351 AA 
Protein Sequence
MPGSAAKGSE LSERIESFVE TLKRGGGPRS SEEMARETLG LLRQIITDHR WSNAGELMEL 60
IRREGRRMTA AQPSETTVGN MVRRVLKIIR EEYGRLHGRS DESDQQESLH KLLTSGGLNE 120
DFSFHYAQLQ SNIIEAINEL LVELEGTMEN IAAQALEHIH SNEVIMTIGF SRTVEAFLKE 180
AARKRKFHVI VAECAPFCQG HEMAVNLSKA GIETTVMTDA AIFAVMSRVN KVIIGTKTIL 240
ANGALRAVTG THTLALAAKH HSTPLIVCAP MFKLSPQFPN EEDSFHKFVA PEEVLPFTEG 300
DILEKVSVHC PVFDYVPPEL ITLFISNIGG NAPSYIYRLM SELYHPDDHV L 351 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005851; C:eukaryotic translation initiation factor 2B complex; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IDA:UniProtKB.
 GO:0005525; F:GTP binding; IDA:UniProtKB.
 GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-KW.
 GO:0051716; P:cellular response to stimulus; IDA:UniProtKB.
 GO:0042552; P:myelination; IMP:UniProtKB.
 GO:0014003; P:oligodendrocyte development; IMP:UniProtKB.
 GO:0001541; P:ovarian follicle development; IMP:UniProtKB.
 GO:0006446; P:regulation of translational initiation; TAS:UniProtKB.
 GO:0009749; P:response to glucose stimulus; ISS:UniProtKB.
 GO:0009408; P:response to heat; ISS:UniProtKB.
 GO:0043434; P:response to peptide hormone stimulus; ISS:UniProtKB.
 GO:0006413; P:translational initiation; IDA:UniProtKB. 
Interpro
 IPR000649; IF-2B-related. 
Pfam
 PF01008; IF-2B 
SMART
  
PROSITE
  
PRINTS