CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000966
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein diaphanous homolog 1 
Protein Synonyms/Alias
 Diaphanous-related formin-1; DRF1 
Gene Name
 DIAPH1 
Gene Synonyms/Alias
 DIAP1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
486AKAAELEKKLDSELTubiquitination[1, 2]
503HELQVEMKKMESDFEubiquitination[3]
774LTPKKLYKPEVQLRRubiquitination[2, 4]
1057VSAENLQKNLDQMKKacetylation[5]
1057VSAENLQKNLDQMKKubiquitination[2, 4]
1103DAQEQYNKLRMMHSNacetylation[5]
1239LLASELTKDDAMAAVubiquitination[3]
Reference
 [1] Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry.
 Denis NJ, Vasilescu J, Lambert JP, Smith JC, Figeys D.
 Proteomics. 2007 Mar;7(6):868-74. [PMID: 17370265]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 Acts in a Rho-dependent manner to recruit PFY1 to the membrane. Required for the assembly of F-actin structures, such as actin cables and stress fibers. Nucleates actin filaments. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization. Required for cytokinesis, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration (By similarity). Has neurite outgrowth promoting activity (By similarity). In hear cells, it may play a role in the regulation of actin polymerization in hair cells. The MEMO1-RHOA- DIAPH1 signaling pathway plays an important role in ERBB2- dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape. 
Sequence Annotation
 DOMAIN 84 449 GBD/FH3.
 DOMAIN 583 764 FH1.
 DOMAIN 769 1171 FH2.
 DOMAIN 1194 1222 DAD.
 MOD_RES 22 22 Phosphoserine.
 MOD_RES 1057 1057 N6-acetyllysine.
 MOD_RES 1103 1103 N6-acetyllysine.
 MOD_RES 1121 1121 Phosphotyrosine (By similarity).
 CROSSLNK 486 486 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Acetylation; Actin-binding; Alternative splicing; Cell membrane; Cell projection; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Deafness; Direct protein sequencing; Hearing; Isopeptide bond; Membrane; Non-syndromic deafness; Phosphoprotein; Reference proteome; Repeat; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1272 AA 
Protein Sequence
MEPPGGSLGP GRGTRDKKKG RSPDELPSAG GDGGKSKKFT LKRLMADELE RFTSMRIKKE 60
KEKPNSAHRN SSASYGDDPT AQSLQDVSDE QVLVLFEQML LDMNLNEEKQ QPLREKDIII 120
KREMVSQYLY TSKAGMSQKE SSKSAMMYIQ ELRSGLRDMP LLSCLESLRV SLNNNPVSWV 180
QTFGAEGLAS LLDILKRLHD EKEETAGSYD SRNKHEIIRC LKAFMNNKFG IKTMLETEEG 240
ILLLVRAMDP AVPNMMIDAA KLLSALCILP QPEDMNERVL EAMTERAEMD EVERFQPLLD 300
GLKSGTTIAL KVGCLQLINA LITPAEELDF RVHIRSELMR LGLHQVLQDL REIENEDMRV 360
QLNVFDEQGE EDSYDLKGRL DDIRMEMDDF NEVFQILLNT VKDSKAEPHF LSILQHLLLV 420
RNDYEARPQY YKLIEECISQ IVLHKNGADP DFKCRHLQIE IEGLIDQMID KTKVEKSEAK 480
AAELEKKLDS ELTARHELQV EMKKMESDFE QKLQDLQGEK DALHSEKQQI ATEKQDLEAE 540
VSQLTGEVAK LTKELEDAKK EMASLSAAAI TVPPSVPSRA PVPPAPPLPG DSGTIIPPPP 600
APGDSTTPPP PPPPPPPPPP LPGGVCISSP PSLPGGTAIS PPPPLSGDAT IPPPPPLPEG 660
VGIPSPSSLP GGTAIPPPPP LPGSARIPPP PPPLPGSAGI PPPPPPLPGE AGMPPPPPPL 720
PGGPGIPPPP PFPGGPGIPP PPPGMGMPPP PPFGFGVPAA PVLPFGLTPK KLYKPEVQLR 780
RPNWSKLVAE DLSQDCFWTK VKEDRFENNE LFAKLTLTFS AQTKTSKAKK DQEGGEEKKS 840
VQKKKVKELK VLDSKTAQNL SIFLGSFRMP YQEIKNVILE VNEAVLTESM IQNLIKQMPE 900
PEQLKMLSEL KDEYDDLAES EQFGVVMGTV PRLRPRLNAI LFKLQFSEQV ENIKPEIVSV 960
TAACEELRKS ESFSNLLEIT LLVGNYMNAG SRNAGAFGFN ISFLCKLRDT KSTDQKMTLL 1020
HFLAELCEND YPDVLKFPDE LAHVEKASRV SAENLQKNLD QMKKQISDVE RDVQNFPAAT 1080
DEKDKFVEKM TSFVKDAQEQ YNKLRMMHSN METLYKELGE YFLFDPKKLS VEEFFMDLHN 1140
FRNMFLQAVK ENQKRRETEE KMRRAKLAKE KAEKERLEKQ QKREQLIDMN AEGDETGVMD 1200
SLLEALQSGA AFRRKRGPRQ ANRKAGCAVT SLLASELTKD DAMAAVPAKV SKNSETFPTI 1260
LEEAKELVGR AS 1272 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0072686; C:mitotic spindle; IDA:BHF-UCL.
 GO:0043005; C:neuron projection; IEA:Compara.
 GO:0032587; C:ruffle membrane; IEA:UniProtKB-SubCell.
 GO:0005102; F:receptor binding; NAS:ProtInc.
 GO:0030041; P:actin filament polymerization; IEA:Compara.
 GO:0071420; P:cellular response to histamine; IMP:BHF-UCL.
 GO:0007010; P:cytoskeleton organization; IMP:UniProtKB.
 GO:0031175; P:neuron projection development; IEA:Compara.
 GO:0035372; P:protein localization to microtubule; IMP:BHF-UCL.
 GO:0008360; P:regulation of cell shape; IMP:UniProtKB.
 GO:0032886; P:regulation of microtubule-based process; IMP:UniProtKB.
 GO:0051279; P:regulation of release of sequestered calcium ion into cytosol; IMP:BHF-UCL.
 GO:0007605; P:sensory perception of sound; TAS:ProtInc. 
Interpro
 IPR003104; Actin-bd_FH2/DRF_autoreg.
 IPR016024; ARM-type_fold.
 IPR014767; Diaphanous_autoregulatory.
 IPR010465; Drf_DAD.
 IPR010472; Drf_FH3.
 IPR010473; Drf_GTPase-bd.
 IPR015425; FH2_actin-bd.
 IPR009408; Formin_homology_1.
 IPR014768; GTPase-bd/formin_homology_3. 
Pfam
 PF06345; Drf_DAD
 PF06346; Drf_FH1
 PF06367; Drf_FH3
 PF06371; Drf_GBD
 PF02181; FH2 
SMART
 SM00498; FH2 
PROSITE
 PS51231; DAD
 PS51444; FH2
 PS51232; GBD_FH3 
PRINTS