UniProt Accession

 SPPL3_HUMAN; Q8TCT6; Q3MJ04; Q8TAU4; Q96DD9 

Genbank Protein ID

 AJ345030; AJ420898; AY169313; AB252457; CH471054; BC009551; BC025781; BC073910; BC101625; BC101627; AK074916 

Genbank Nucleotide ID

 CAC87791.1; CAD13135.1; AAO12538.1; BAF30928.1; EAW98220.1; AAH09551.1; AAH25781.1; AAH73910.1; AAI01626.1; AAI01628.1; BAC11290.1 

Protein Name

 Signal peptide peptidase-like 3 

Protein Synonyms/Alias

 SPP-like 3; Intramembrane protease 2; IMP-2; Presenilin homologous protein 1; PSH1; Presenilin-like protein 4 

Gene Name

 SPPL3 

Gene Synonyms/Alias

 IMP2; PSL4 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
235	PLDVLSRKLHLGPNV	ubiquitination	[1]
290	VLRYDNYKKQASGDS	ubiquitination	[1, 2]
291	LRYDNYKKQASGDSC	ubiquitination	[1, 2]
376	WSEPFHSKSSSSRFL	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 

Functional Description

 Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane. 

Sequence Annotation

 MOTIF 342 344 PAL.
 ACT_SITE 201 201 By similarity.
 ACT_SITE 272 272 By similarity.  

Keyword

 Alternative splicing; Complete proteome; Endoplasmic reticulum; Hydrolase; Membrane; Protease; Reference proteome; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 385 AA 

Protein Sequence

Gene Ontology

 GO:0030660; C:Golgi-associated vesicle membrane; IDA:UniProtKB.
 GO:0071458; C:integral to cytosolic side of endoplasmic reticulum membrane; IDA:UniProtKB.
 GO:0071556; C:integral to lumenal side of endoplasmic reticulum membrane; IDA:UniProtKB.
 GO:0005791; C:rough endoplasmic reticulum; IDA:UniProtKB.
 GO:0042500; F:aspartic endopeptidase activity, intramembrane cleaving; IMP:UniProtKB.
 GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 

Interpro

 IPR006639; Peptidase_A22.
 IPR007369; Peptidase_A22B_SPP. 

Pfam

 PF04258; Peptidase_A22B 

SMART

 SM00730; PSN 

PROSITE

  

PRINTS