Tag | Content |
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CPLM ID | CPLM-019546 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Serine/threonine-protein kinase SMG1 |
Protein Synonyms/Alias | SMG-1; hSMG-1; 61E3.4; Lambda/iota protein kinase C-interacting protein; Lambda-interacting protein |
Gene Name | SMG1 |
Gene Synonyms/Alias | ATX; KIAA0421; LIP |
Created Date | July 27, 2013 |
Organism | Homo sapiens (Human) |
NCBI Taxa ID | 9606 |
Lysine Modification | Position | Peptide | Type | References |
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389 | KLFIPSSKLLFLRYH | ubiquitination | [1] | 2092 | EAALQAQKAQDSYQT | ubiquitination | [1] |
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Reference | [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization. Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW. Nature. 2013 Apr 18;496(7445):372-6. [ PMID: 23503661] |
Functional Description | Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate p53/TP53 and is required for optimal p53/TP53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR). May activate PRKCI but not PRKCZ. |
Sequence Annotation | DOMAIN 1131 1866 FAT. REPEAT 1817 1852 HEAT. DOMAIN 2150 2478 PI3K/PI4K. DOMAIN 3629 3661 FATC. REGION 1 1977 Interaction with SMG8 and SMG9. MOD_RES 173 173 N6-acetyllysine. MOD_RES 3550 3550 Phosphothreonine. MOD_RES 3556 3556 Phosphoserine. MOD_RES 3570 3570 Phosphoserine. MOD_RES 3573 3573 Phosphothreonine. MOD_RES 3577 3577 Phosphothreonine (By similarity). |
Keyword | Acetylation; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; DNA damage; DNA repair; Kinase; Manganese; Metal-binding; Nonsense-mediated mRNA decay; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 3661 AA |
Protein Sequence | MSYSDESRLS NLLRRITRED DRDRRLATVK QLKEFIQQPE NKLVLVKQLD NILAAVHDVL 60 NESSKLLQEL RQEGACCLGL LCASLSYEAE KIFKWIFSKF SSSAKDEVKL LYLCATYKAL 120 ETVGEKKAFS SVMQLVMTSL QSILENVDTP ELLCKCVKCI LLVARCYPHI FSTNFRDTVD 180 ILVGWHIDHT QKPSLTQQVS GWLQSLEPFW VADLAFSTTL LGQFLEDMEA YAEDLSHVAS 240 GESVDEDVPP PSVSLPKLAA LLRVFSTVVR SIGERFSPIR GPPITEAYVT DVLYRVMRCV 300 TAANQVFFSE AVLTAANECV GVLLGSLDPS MTIHCDMVIT YGLDQLENCQ TCGTDYIISV 360 LNLLTLIVEQ INTKLPSSFV EKLFIPSSKL LFLRYHKEKE VVAVAHAVYQ AVLSLKNIPV 420 LETAYKLILG EMTCALNNLL HSLQLPEACS EIKHEAFKNH VFNVDNAKFV VIFDLSALTT 480 IGNAKNSLIG MWALSPTVFA LLSKNLMIVH SDLAVHFPAI QYAVLYTLYS HCTRHDHFIS 540 SSLSSSSPSL FDGAVISTVT TATKKHFSII LNLLGILLKK DNLNQDTRKL LMTWALEAAV 600 LMKKSETYAP LFSLPSFHKF CKGLLANTLV EDVNICLQAC SSLHALSSSL PDDLLQRCVD 660 VCRVQLVHSG TRIRQAFGKL LKSIPLDVVL SNNNHTEIQE ISLALRSHMS KAPSNTFHPQ 720 DFSDVISFIL YGNSHRTGKD NWLERLFYSC QRLDKRDQST IPRNLLKTDA VLWQWAIWEA 780 AQFTVLSKLR TPLGRAQDTF QTIEGIIRSL AAHTLNPDQD VSQWTTADND EGHGNNQLRL 840 VLLLQYLENL EKLMYNAYEG CANALTSPPK VIRTFFYTNR QTCQDWLTRI RLSIMRVGLL 900 AGQPAVTVRH GFDLLTEMKT TSLSQGNELE VTIMMVVEAL CELHCPEAIQ GIAVWSSSIV 960 GKNLLWINSV AQQAEGRFEK ASVEYQEHLC AMTGVDCCIS SFDKSVLTLA NAGRNSASPK 1020 HSLNGESRKT VLSKPTDSSP EVINYLGNKA CECYISIADW AAVQEWQNAI HDLKKSTSST 1080 SLNLKADFNY IKSLSSFESG KFVECTEQLE LLPGENINLL AGGSKEKIDM KKLLPNMLSP 1140 DPRELQKSIE VQLLRSSVCL ATALNPIEQD QKWQSITENV VKYLKQTSRI AIGPLRLSTL 1200 TVSQSLPVLS TLQLYCSSAL ENTVSNRLST EDCLIPLFSE ALRSCKQHDV RPWMQALRYT 1260 MYQNQLLEKI KEQTVPIRSH LMELGLTAAK FARKRGNVSL ATRLLAQCSE VQLGKTTTAQ 1320 DLVQHFKKLS TQGQVDEKWG PELDIEKTKL LYTAGQSTHA MEMLSSCAIS FCKSVKAEYA 1380 VAKSILTLAK WIQAEWKEIS GQLKQVYRAQ HQQNFTGLST LSKNILTLIE LPSVNTMEEE 1440 YPRIESESTV HIGVGEPDFI LGQLYHLSSV QAPEVAKSWA ALASWAYRWG RKVVDNASQG 1500 EGVRLLPREK SEVQNLLPDT ITEEEKERIY GILGQAVCRP AGIQDEDITL QITESEDNEE 1560 DDMVDVIWRQ LISSCPWLSE LDESATEGVI KVWRKVVDRI FSLYKLSCSA YFTFLKLNAG 1620 QIPLDEDDPR LHLSHRVEQS TDDMIVMATL RLLRLLVKHA GELRQYLEHG LETTPTAPWR 1680 GIIPQLFSRL NHPEVYVRQS ICNLLCRVAQ DSPHLILYPA IVGTISLSSE SQASGNKFST 1740 AIPTLLGNIQ GEELLVSECE GGSPPASQDS NKDEPKSGLN EDQAMMQDCY SKIVDKLSSA 1800 NPTMVLQVQM LVAELRRVTV LWDELWLGVL LQQHMYVLRR IQQLEDEVKR VQNNNTLRKE 1860 EKIAIMREKH TALMKPIVFA LEHVRSITAA PAETPHEKWF QDNYGDAIEN ALEKLKTPLN 1920 PAKPGSSWIP FKEIMLSLQQ RAQKRASYIL RLEEISPWLA AMTNTEIALP GEVSARDTVT 1980 IHSVGGTITI LPTKTKPKKL LFLGSDGKSY PYLFKGLEDL HLDERIMQFL SIVNTMFATI 2040 NRQETPRFHA RHYSVTPLGT RSGLIQWVDG ATPLFGLYKR WQQREAALQA QKAQDSYQTP 2100 QNPGIVPRPS ELYYSKIGPA LKTVGLSLDV SRRDWPLHVM KAVLEELMEA TPPNLLAKEL 2160 WSSCTTPDEW WRVTQSYARS TAVMSMVGYI IGLGDRHLDN VLIDMTTGEV VHIDYNVCFE 2220 KGKSLRVPEK VPFRMTQNIE TALGVTGVEG VFRLSCEQVL HIMRRGRETL LTLLEAFVYD 2280 PLVDWTAGGE AGFAGAVYGG GGQQAESKQS KREMEREITR SLFSSRVAEI KVNWFKNRDE 2340 MLVVLPKLDG SLDEYLSLQE QLTDVEKLQG KLLEEIEFLE GAEGVDHPSH TLQHRYSEHT 2400 QLQTQQRAVQ EAIQVKLNEF EQWITHYQAA FNNLEATQLA SLLQEISTQM DLGPPSYVPA 2460 TAFLQNAGQA HLISQCEQLE GEVGALLQQR RSVLRGCLEQ LHHYATVALQ YPKAIFQKHR 2520 IEQWKTWMEE LICNTTVERC QELYRKYEMQ YAPQPPPTVC QFITATEMTL QRYAADINSR 2580 LIRQVERLKQ EAVTVPVCED QLKEIERCIK VFLHENGEEG SLSLASVIIS ALCTLTRRNL 2640 MMEGAASSAG EQLVDLTSRD GAWFLEELCS MSGNVTCLVQ LLKQCHLVPQ DLDIPNPMEA 2700 SETVHLANGV YTSLQELNSN FRQIIFPEAL RCLMKGEYTL ESMLHELDGL IEQTTDGVPL 2760 QTLVESLQAY LRNAAMGLEE ETHAHYIDVA RLLHAQYGEL IQPRNGSVDE TPKMSAGQML 2820 LVAFDGMFAQ VETAFSLLVE KLNKMEIPIA WRKIDIIREA RSTQVNFFDD DNHRQVLEEI 2880 FFLKRLQTIK EFFRLCGTFS KTLSGSSSLE DQNTVNGPVQ IVNVKTLFRN SCFSEDQMAK 2940 PIKAFTADFV RQLLIGLPNQ ALGLTLCSFI SALGVDIIAQ VEAKDFGAES KVSVDDLCKK 3000 AVEHNIQIGK FSQLVMNRAT VLASSYDTAW KKHDLVRRLE TSISSCKTSL QRVQLHIAMF 3060 QWQHEDLLIN RPQAMSVTPP PRSAILTSMK KKLHTLSQIE TSIATVQEKL AALESSIEQR 3120 LKWAGGANPA LAPVLQDFEA TIAERRNLVL KESQRASQVT FLCSNIIHFE SLRTRTAEAL 3180 NLDAALFELI KRCQQMCSFA SQFNSSVSEL ELRLLQRVDT GLEHPIGSSE WLLSAHKQLT 3240 QDMSTQRAIQ TEKEQQIETV CETIQNLVDN IKTVLTGHNR QLGDVKHLLK AMAKDEEAAL 3300 ADGEDVPYEN SVRQFLGEYK SWQDNIQTVL FTLVQAMGQV RSQEHVEMLQ EITPTLKELK 3360 TQSQSIYNNL VSFASPLVTD ATNECSSPTS SATYQPSFAA AVRSNTGQKT QPDVMSQNAR 3420 KLIQKNLATS ADTPPSTVPG TGKSVACSPK KAVRDPKTGK AVQERNSYAV SVWKRVKAKL 3480 EGRDVDPNRR MSVAEQVDYV IKEATNLDNL AQLYEGWTAW V 3521 |
Gene Ontology | GO:0005829; C:cytosol; TAS:Reactome. GO:0005634; C:nucleus; IDA:UniProtKB. GO:0005524; F:ATP binding; IEA:UniProtKB-KW. GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. GO:0004674; F:protein serine/threonine kinase activity; IDA:UniProtKB. GO:0006281; P:DNA repair; IEA:UniProtKB-KW. GO:0010467; P:gene expression; TAS:Reactome. GO:0006406; P:mRNA export from nucleus; TAS:UniProtKB. GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IDA:UniProtKB. GO:0018105; P:peptidyl-serine phosphorylation; IDA:UniProtKB. GO:0046854; P:phosphatidylinositol phosphorylation; IDA:UniProtKB. GO:0046777; P:protein autophosphorylation; IDA:UniProtKB. GO:0006950; P:response to stress; IDA:UniProtKB. |
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