CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019546
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein kinase SMG1 
Protein Synonyms/Alias
 SMG-1; hSMG-1; 61E3.4; Lambda/iota protein kinase C-interacting protein; Lambda-interacting protein 
Gene Name
 SMG1 
Gene Synonyms/Alias
 ATX; KIAA0421; LIP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
389KLFIPSSKLLFLRYHubiquitination[1]
2092EAALQAQKAQDSYQTubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
 Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate p53/TP53 and is required for optimal p53/TP53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR). May activate PRKCI but not PRKCZ. 
Sequence Annotation
 DOMAIN 1131 1866 FAT.
 REPEAT 1817 1852 HEAT.
 DOMAIN 2150 2478 PI3K/PI4K.
 DOMAIN 3629 3661 FATC.
 REGION 1 1977 Interaction with SMG8 and SMG9.
 MOD_RES 173 173 N6-acetyllysine.
 MOD_RES 3550 3550 Phosphothreonine.
 MOD_RES 3556 3556 Phosphoserine.
 MOD_RES 3570 3570 Phosphoserine.
 MOD_RES 3573 3573 Phosphothreonine.
 MOD_RES 3577 3577 Phosphothreonine (By similarity).  
Keyword
 Acetylation; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; DNA damage; DNA repair; Kinase; Manganese; Metal-binding; Nonsense-mediated mRNA decay; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 3661 AA 
Protein Sequence
MSYSDESRLS NLLRRITRED DRDRRLATVK QLKEFIQQPE NKLVLVKQLD NILAAVHDVL 60
NESSKLLQEL RQEGACCLGL LCASLSYEAE KIFKWIFSKF SSSAKDEVKL LYLCATYKAL 120
ETVGEKKAFS SVMQLVMTSL QSILENVDTP ELLCKCVKCI LLVARCYPHI FSTNFRDTVD 180
ILVGWHIDHT QKPSLTQQVS GWLQSLEPFW VADLAFSTTL LGQFLEDMEA YAEDLSHVAS 240
GESVDEDVPP PSVSLPKLAA LLRVFSTVVR SIGERFSPIR GPPITEAYVT DVLYRVMRCV 300
TAANQVFFSE AVLTAANECV GVLLGSLDPS MTIHCDMVIT YGLDQLENCQ TCGTDYIISV 360
LNLLTLIVEQ INTKLPSSFV EKLFIPSSKL LFLRYHKEKE VVAVAHAVYQ AVLSLKNIPV 420
LETAYKLILG EMTCALNNLL HSLQLPEACS EIKHEAFKNH VFNVDNAKFV VIFDLSALTT 480
IGNAKNSLIG MWALSPTVFA LLSKNLMIVH SDLAVHFPAI QYAVLYTLYS HCTRHDHFIS 540
SSLSSSSPSL FDGAVISTVT TATKKHFSII LNLLGILLKK DNLNQDTRKL LMTWALEAAV 600
LMKKSETYAP LFSLPSFHKF CKGLLANTLV EDVNICLQAC SSLHALSSSL PDDLLQRCVD 660
VCRVQLVHSG TRIRQAFGKL LKSIPLDVVL SNNNHTEIQE ISLALRSHMS KAPSNTFHPQ 720
DFSDVISFIL YGNSHRTGKD NWLERLFYSC QRLDKRDQST IPRNLLKTDA VLWQWAIWEA 780
AQFTVLSKLR TPLGRAQDTF QTIEGIIRSL AAHTLNPDQD VSQWTTADND EGHGNNQLRL 840
VLLLQYLENL EKLMYNAYEG CANALTSPPK VIRTFFYTNR QTCQDWLTRI RLSIMRVGLL 900
AGQPAVTVRH GFDLLTEMKT TSLSQGNELE VTIMMVVEAL CELHCPEAIQ GIAVWSSSIV 960
GKNLLWINSV AQQAEGRFEK ASVEYQEHLC AMTGVDCCIS SFDKSVLTLA NAGRNSASPK 1020
HSLNGESRKT VLSKPTDSSP EVINYLGNKA CECYISIADW AAVQEWQNAI HDLKKSTSST 1080
SLNLKADFNY IKSLSSFESG KFVECTEQLE LLPGENINLL AGGSKEKIDM KKLLPNMLSP 1140
DPRELQKSIE VQLLRSSVCL ATALNPIEQD QKWQSITENV VKYLKQTSRI AIGPLRLSTL 1200
TVSQSLPVLS TLQLYCSSAL ENTVSNRLST EDCLIPLFSE ALRSCKQHDV RPWMQALRYT 1260
MYQNQLLEKI KEQTVPIRSH LMELGLTAAK FARKRGNVSL ATRLLAQCSE VQLGKTTTAQ 1320
DLVQHFKKLS TQGQVDEKWG PELDIEKTKL LYTAGQSTHA MEMLSSCAIS FCKSVKAEYA 1380
VAKSILTLAK WIQAEWKEIS GQLKQVYRAQ HQQNFTGLST LSKNILTLIE LPSVNTMEEE 1440
YPRIESESTV HIGVGEPDFI LGQLYHLSSV QAPEVAKSWA ALASWAYRWG RKVVDNASQG 1500
EGVRLLPREK SEVQNLLPDT ITEEEKERIY GILGQAVCRP AGIQDEDITL QITESEDNEE 1560
DDMVDVIWRQ LISSCPWLSE LDESATEGVI KVWRKVVDRI FSLYKLSCSA YFTFLKLNAG 1620
QIPLDEDDPR LHLSHRVEQS TDDMIVMATL RLLRLLVKHA GELRQYLEHG LETTPTAPWR 1680
GIIPQLFSRL NHPEVYVRQS ICNLLCRVAQ DSPHLILYPA IVGTISLSSE SQASGNKFST 1740
AIPTLLGNIQ GEELLVSECE GGSPPASQDS NKDEPKSGLN EDQAMMQDCY SKIVDKLSSA 1800
NPTMVLQVQM LVAELRRVTV LWDELWLGVL LQQHMYVLRR IQQLEDEVKR VQNNNTLRKE 1860
EKIAIMREKH TALMKPIVFA LEHVRSITAA PAETPHEKWF QDNYGDAIEN ALEKLKTPLN 1920
PAKPGSSWIP FKEIMLSLQQ RAQKRASYIL RLEEISPWLA AMTNTEIALP GEVSARDTVT 1980
IHSVGGTITI LPTKTKPKKL LFLGSDGKSY PYLFKGLEDL HLDERIMQFL SIVNTMFATI 2040
NRQETPRFHA RHYSVTPLGT RSGLIQWVDG ATPLFGLYKR WQQREAALQA QKAQDSYQTP 2100
QNPGIVPRPS ELYYSKIGPA LKTVGLSLDV SRRDWPLHVM KAVLEELMEA TPPNLLAKEL 2160
WSSCTTPDEW WRVTQSYARS TAVMSMVGYI IGLGDRHLDN VLIDMTTGEV VHIDYNVCFE 2220
KGKSLRVPEK VPFRMTQNIE TALGVTGVEG VFRLSCEQVL HIMRRGRETL LTLLEAFVYD 2280
PLVDWTAGGE AGFAGAVYGG GGQQAESKQS KREMEREITR SLFSSRVAEI KVNWFKNRDE 2340
MLVVLPKLDG SLDEYLSLQE QLTDVEKLQG KLLEEIEFLE GAEGVDHPSH TLQHRYSEHT 2400
QLQTQQRAVQ EAIQVKLNEF EQWITHYQAA FNNLEATQLA SLLQEISTQM DLGPPSYVPA 2460
TAFLQNAGQA HLISQCEQLE GEVGALLQQR RSVLRGCLEQ LHHYATVALQ YPKAIFQKHR 2520
IEQWKTWMEE LICNTTVERC QELYRKYEMQ YAPQPPPTVC QFITATEMTL QRYAADINSR 2580
LIRQVERLKQ EAVTVPVCED QLKEIERCIK VFLHENGEEG SLSLASVIIS ALCTLTRRNL 2640
MMEGAASSAG EQLVDLTSRD GAWFLEELCS MSGNVTCLVQ LLKQCHLVPQ DLDIPNPMEA 2700
SETVHLANGV YTSLQELNSN FRQIIFPEAL RCLMKGEYTL ESMLHELDGL IEQTTDGVPL 2760
QTLVESLQAY LRNAAMGLEE ETHAHYIDVA RLLHAQYGEL IQPRNGSVDE TPKMSAGQML 2820
LVAFDGMFAQ VETAFSLLVE KLNKMEIPIA WRKIDIIREA RSTQVNFFDD DNHRQVLEEI 2880
FFLKRLQTIK EFFRLCGTFS KTLSGSSSLE DQNTVNGPVQ IVNVKTLFRN SCFSEDQMAK 2940
PIKAFTADFV RQLLIGLPNQ ALGLTLCSFI SALGVDIIAQ VEAKDFGAES KVSVDDLCKK 3000
AVEHNIQIGK FSQLVMNRAT VLASSYDTAW KKHDLVRRLE TSISSCKTSL QRVQLHIAMF 3060
QWQHEDLLIN RPQAMSVTPP PRSAILTSMK KKLHTLSQIE TSIATVQEKL AALESSIEQR 3120
LKWAGGANPA LAPVLQDFEA TIAERRNLVL KESQRASQVT FLCSNIIHFE SLRTRTAEAL 3180
NLDAALFELI KRCQQMCSFA SQFNSSVSEL ELRLLQRVDT GLEHPIGSSE WLLSAHKQLT 3240
QDMSTQRAIQ TEKEQQIETV CETIQNLVDN IKTVLTGHNR QLGDVKHLLK AMAKDEEAAL 3300
ADGEDVPYEN SVRQFLGEYK SWQDNIQTVL FTLVQAMGQV RSQEHVEMLQ EITPTLKELK 3360
TQSQSIYNNL VSFASPLVTD ATNECSSPTS SATYQPSFAA AVRSNTGQKT QPDVMSQNAR 3420
KLIQKNLATS ADTPPSTVPG TGKSVACSPK KAVRDPKTGK AVQERNSYAV SVWKRVKAKL 3480
EGRDVDPNRR MSVAEQVDYV IKEATNLDNL AQLYEGWTAW V 3521 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:UniProtKB.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:0010467; P:gene expression; TAS:Reactome.
 GO:0006406; P:mRNA export from nucleus; TAS:UniProtKB.
 GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IDA:UniProtKB.
 GO:0018105; P:peptidyl-serine phosphorylation; IDA:UniProtKB.
 GO:0046854; P:phosphatidylinositol phosphorylation; IDA:UniProtKB.
 GO:0046777; P:protein autophosphorylation; IDA:UniProtKB.
 GO:0006950; P:response to stress; IDA:UniProtKB. 
Interpro
 IPR016024; ARM-type_fold.
 IPR003152; FATC.
 IPR011009; Kinase-like_dom.
 IPR000403; PI3/4_kinase_cat_dom.
 IPR018936; PI3/4_kinase_CS.
 IPR014009; PIK_FAT. 
Pfam
 PF02260; FATC
 PF00454; PI3_PI4_kinase 
SMART
 SM00146; PI3Kc 
PROSITE
 PS51189; FAT
 PS51190; FATC
 PS50077; HEAT_REPEAT
 PS00915; PI3_4_KINASE_1
 PS00916; PI3_4_KINASE_2
 PS50290; PI3_4_KINASE_3 
PRINTS