CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031184
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 SFRS protein kinase 1, isoform CRA_f 
Protein Synonyms/Alias
 SRSF protein kinase 1; cDNA FLJ58405, highly similar to Serine/threonine-protein kinase SRPK1 (EC 2.7.11.1) 
Gene Name
 SRPK1 
Gene Synonyms/Alias
 hCG_15775 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
268RQAELLEKRMQEIEEubiquitination[1]
456GPLDNKGKSTAGNFLubiquitination[1]
478NAEKLKVKIADLGNAubiquitination[1, 2]
563LLGKVPRKLIVAGKYubiquitination[1]
569RKLIVAGKYSKEFFTacetylation[3]
569RKLIVAGKYSKEFFTubiquitination[1, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 639 AA 
Protein Sequence
MGIFVSFLRS ETQHRGSAPH SESDLPEQEE EILGSDDDEQ EDPNDYCKGG YHLVKIGDLF 60
NGRYHVIRKL GWGHFSTVWL SWDIQGKKFV AMKVVKSAEH YTETALDEIR LLKSVRNSDP 120
NDPNREMVVQ LLDDFKISGV NGTHICMVFE VLGHHLLKWI IKSNYQGLPL PCVKKIIQQV 180
LQGLDYLHTK CRIIHTDIKP ENILLSVNEQ YIRRLAAEAT EWQRSGAPPP SGSAVSTAPQ 240
PKPADKMSKN KKKKLKKKQK RQAELLEKRM QEIEEMEKES GPGQKRPNKQ EESESPVERP 300
LKENPPNKMT QEKLEESSTI GQDQTLMERD TEGGAAEINC NGVIEVINYT QNSNNETLRH 360
KEDLHNANDC DVQNLNQESS FLSSQNGDSS TSQETDSCTP ITSEVSDTMV CQSSSTVGQS 420
FSEQHISQLQ ESIRAEIPCE DEQEQEHNGP LDNKGKSTAG NFLVNPLEPK NAEKLKVKIA 480
DLGNACWVHK HFTEDIQTRQ YRSLEVLIGS GYNTPADIWS TACMAFELAT GDYLFEPHSG 540
EEYTRDEDHI ALIIELLGKV PRKLIVAGKY SKEFFTKKGD LKHITKLKPW GLFEVLVEKY 600
EWSQEEAAGF TDFLLPMLEL IPEKRATAAE CLRHPWLNS 639 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
  
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS