| Tag | Content |
|---|
| CPLM ID | CPLM-019090 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Alkylated DNA repair protein alkB homolog 8 |
Protein Synonyms/Alias | Probable alpha-ketoglutarate-dependent dioxygenase ABH8; S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8; tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ABH8 |
Gene Name | ALKBH8 |
Gene Synonyms/Alias | ABH8 |
Created Date | July 27, 2013 |
Organism | Homo sapiens (Human) |
NCBI Taxa ID | 9606 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 419 | DIGCGNGKYLGINKE | ubiquitination | [1] |
|
Reference | [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments. Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA. Mol Cell Proteomics. 2013 Mar;12(3):825-31. [ PMID: 23266961] |
Functional Description | Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA. Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA. Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys). Required for normal survival after DNA damage. May inhibit apoptosis and promote cell survival and angiogenesis. |
Sequence Annotation | DOMAIN 43 120 RRM.
DOMAIN 220 337 Fe2OG dioxygenase.
REGION 227 229 Alpha-ketoglutarate binding (By
REGION 328 334 Alpha-ketoglutarate binding (By
REGION 411 664 Methyltransferase domain.
METAL 238 238 Iron; catalytic (By similarity).
METAL 240 240 Iron; catalytic (By similarity).
METAL 292 292 Iron; catalytic (By similarity). |
Keyword | 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Iron; Metal-binding; Methyltransferase; Multifunctional enzyme; Nucleus; Oxidoreductase; Reference proteome; RNA-binding; S-adenosyl-L-methionine; Transferase. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 664 AA |
Protein Sequence | MDSNHQSNYK LSKTEKKFLR KQIKAKHTLL RHEGIETVSY ATQSLVVANG GLGNGVSRNQ 60
LLPVLEKCGL VDALLMPPNK PYSFARYRTT EESKRAYVTL NGKEVVDDLG QKITLYLNFV 120
EKVQWKELRP QALPPGLMVV EEIISSEEEK MLLESVDWTE DTDNQNSQKS LKHRRVKHFG 180
YEFHYENNNV DKDKPLSGGL PDICESFLEK WLRKGYIKHK PDQMTINQYE PGQGIPAHID 240
THSAFEDEIV SLSLGSEIVM DFKHPDGIAV PVMLPRRSLL VMTGESRYLW THGITCRKFD 300
TVQASESLKS GIITSDVGDL TLSKRGLRTS FTFRKVRQTP CNCSYPLVCD SQRKETPPSF 360
PESDKEASRL EQEYVHQVYE EIAGHFSSTR HTPWPHIVEF LKALPSGSIV ADIGCGNGKY 420
LGINKELYMI GCDRSQNLVD ICRERQFQAF VCDALAVPVR SGSCDACISI AVIHHFATAE 480
RRVAALQEIV RLLRPGGKAL IYVWAMEQEY NKQKSKYLRG NRNSQGKKEE MNSDTSVQRS 540
LVEQMRDMGS RDSASSVPRI NDSQEGGCNS RQVSNSKLPV HVNRTSFYSQ DVLVPWHLKG 600
NPDKGKPVEP FGPIGSQDPS PVFHRYYHVF REGELEGACR TVSDVRILQS YYDQGNWCVI 660
LQKA 664 |
Gene Ontology | GO:0005829; C:cytosol; IDA:UniProtKB. GO:0015630; C:microtubule cytoskeleton; IDA:HPA. GO:0005634; C:nucleus; IDA:UniProtKB. GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. GO:0016706; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; IEA:InterPro. GO:0003723; F:RNA binding; IEA:UniProtKB-KW. GO:0016300; F:tRNA (uracil) methyltransferase activity; IDA:UniProtKB. GO:0006974; P:response to DNA damage stimulus; IDA:UniProtKB. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |