CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014400
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Disks large homolog 4 
Protein Synonyms/Alias
 Postsynaptic density protein 95; PSD-95; Synapse-associated protein 90; SAP-90; SAP90 
Gene Name
 Dlg4 
Gene Synonyms/Alias
 Dlgh4; Psd95 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
299RRYSPVAKDLLGEEDubiquitination[1]
355DLSGELRKGDQILSVubiquitination[1]
403EYSRFEAKIHDLREQubiquitination[1]
663ENVLEINKRITEEQAubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. 
Sequence Annotation
 DOMAIN 65 151 PDZ 1.
 DOMAIN 160 246 PDZ 2.
 DOMAIN 313 393 PDZ 3.
 DOMAIN 428 498 SH3.
 DOMAIN 534 709 Guanylate kinase-like.
 MOD_RES 142 142 Phosphoserine.
 MOD_RES 240 240 Phosphotyrosine.
 MOD_RES 295 295 Phosphoserine.
 MOD_RES 397 397 Phosphotyrosine.
 MOD_RES 415 415 Phosphoserine.
 MOD_RES 418 418 Phosphoserine.
 MOD_RES 432 432 Phosphotyrosine.
 MOD_RES 580 580 Phosphotyrosine.
 MOD_RES 604 604 Phosphotyrosine.
 MOD_RES 715 715 Phosphotyrosine.
 LIPID 3 3 S-palmitoyl cysteine (By similarity).
 LIPID 5 5 S-palmitoyl cysteine (By similarity).  
Keyword
 Alternative splicing; Cell junction; Cell membrane; Complete proteome; Lipoprotein; Membrane; Palmitate; Phosphoprotein; Postsynaptic cell membrane; Reference proteome; Repeat; SH3 domain; Synapse; Synaptosome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 724 AA 
Protein Sequence
MDCLCIVTTK KYRYQDEDTP PLEHSPAHLP NQANSPPVIV NTDTLEAPGY ELQVNGTEGE 60
MEYEEITLER GNSGLGFSIA GGTDNPHIGD DPSIFITKII PGGAAAQDGR LRVNDSILFV 120
NEVDVREVTH SAAVEALKEA GSIVRLYVMR RKPPAEKIIE IKLIKGPKGL GFSIAGGVGN 180
QHIPGDNSIY VTKIIEGGAA HKDGRLQIGD KILAVNSVGL EDVMHEDAVA ALKNTYDVVY 240
LKVAKPSNAY LSDSYAPPDI TTSYSQHLDN EISHSSYLGT DYPTAMTPTS PRRYSPVAKD 300
LLGEEDIPRE PRRIVIHRGS TGLGFNIVGG EDGEGIFISF ILAGGPADLS GELRKGDQIL 360
SVNGVDLRNA SHEQAAIALK NAGQTVTIIA QYKPEEYSRF EAKIHDLREQ LMNSSLGSGT 420
ASLRSNPKRG FYIRALFDYD KTKDCGFLSQ ALSFHFGDVL HVIDASDEEW WQARRVHSDS 480
ETDDIGFIPS KRRVERREWS RLKAKDWGSS SGSQGREDSV LSYETVTQME VHYARPIIIL 540
GPTKDRANDD LLSEFPDKFG SCVPHTTRPK REYEIDGRDY HFVSSREKME KDIQAHKFIE 600
AGQYNSHLYG TSVQSVREVA EQGKHCILDV SANAVRRLQA AHLHPIAIFI RPRSLENVLE 660
INKRITEEQA RKAFDRATKL EQEFTECFSA IVEGDSFEEI YHKVKRVIED LSGPYIWVPA 720
RERL 724 
Gene Ontology
 GO:0032281; C:alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid selective glutamate receptor complex; IDA:MGI.
 GO:0030054; C:cell junction; IDA:MGI.
 GO:0032839; C:dendrite cytoplasm; ISS:BHF-UCL.
 GO:0060076; C:excitatory synapse; IDA:BHF-UCL.
 GO:0031234; C:extrinsic to internal side of plasma membrane; IDA:MGI.
 GO:0044224; C:juxtaparanode region of axon; IDA:MGI.
 GO:0044306; C:neuron projection terminus; IDA:MGI.
 GO:0014069; C:postsynaptic density; ISS:UniProtKB.
 GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-KW.
 GO:0008021; C:synaptic vesicle; IDA:MGI.
 GO:0045032; F:ADP-activated nucleotide receptor activity; ISS:BHF-UCL.
 GO:0045031; F:ATP-activated nucleotide receptor activity; ISS:BHF-UCL.
 GO:0042043; F:neurexin family protein binding; NAS:UniProtKB.
 GO:0031812; F:P2Y1 nucleotide receptor binding; ISS:BHF-UCL.
 GO:0008022; F:protein C-terminus binding; NAS:UniProtKB.
 GO:0005198; F:structural molecule activity; NAS:UniProtKB.
 GO:0097113; P:alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate receptor clustering; ISS:BHF-UCL.
 GO:0060997; P:dendritic spine morphogenesis; IDA:BHF-UCL.
 GO:0045184; P:establishment of protein localization; ISS:BHF-UCL.
 GO:0035641; P:locomotory exploration behavior; IMP:BHF-UCL.
 GO:0002091; P:negative regulation of receptor internalization; IEA:Compara.
 GO:0050885; P:neuromuscular process controlling balance; IMP:BHF-UCL.
 GO:2000463; P:positive regulation of excitatory postsynaptic membrane potential; IEA:Compara.
 GO:0050806; P:positive regulation of synaptic transmission; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; ISS:BHF-UCL.
 GO:0006461; P:protein complex assembly; ISS:BHF-UCL.
 GO:0097120; P:receptor localization to synapse; ISS:BHF-UCL.
 GO:2000821; P:regulation of grooming behavior; IMP:BHF-UCL.
 GO:0048169; P:regulation of long-term neuronal synaptic plasticity; IGI:MGI.
 GO:2000310; P:regulation of N-methyl-D-aspartate selective glutamate receptor activity; IEA:Compara.
 GO:0042220; P:response to cocaine; NAS:UniProtKB.
 GO:0035176; P:social behavior; IMP:BHF-UCL.
 GO:0016188; P:synaptic vesicle maturation; IDA:MGI.
 GO:0071625; P:vocalization behavior; IMP:BHF-UCL. 
Interpro
 IPR008144; Guanylate_kin.
 IPR008145; Guanylate_kin/L-typ_Ca_channel.
 IPR020590; Guanylate_kinase_CS.
 IPR016313; M-assoc_guanylate_kinase.
 IPR019590; MAGUK_PEST_N.
 IPR027417; P-loop_NTPase.
 IPR001478; PDZ.
 IPR019583; PDZ_assoc.
 IPR011511; SH3_2.
 IPR001452; SH3_domain. 
Pfam
 PF00625; Guanylate_kin
 PF10608; MAGUK_N_PEST
 PF00595; PDZ
 PF10600; PDZ_assoc
 PF07653; SH3_2 
SMART
 SM00072; GuKc
 SM00228; PDZ
 SM00326; SH3 
PROSITE
 PS00856; GUANYLATE_KINASE_1
 PS50052; GUANYLATE_KINASE_2
 PS50106; PDZ
 PS50002; SH3 
PRINTS