| Tag | Content |
|---|
| CPLM ID | CPLM-023435 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Histone-lysine N-methyltransferase ash1 |
Protein Synonyms/Alias | Absent small and homeotic disks protein 1; Lysine N-methyltransferase 2H |
Gene Name | ash1 |
Gene Synonyms/Alias | KMT2H; CG8887 |
Created Date | July 27, 2013 |
Organism | Drosophila melanogaster (Fruit fly) |
NCBI Taxa ID | 7227 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 868 | QRRGGAAKQKVSAAK | acetylation | [1] | | 870 | RGGAAKQKVSAAKDL | acetylation | [1] | | 1952 | PIKDESGKVLPTKKH | acetylation | [1] |
|
Reference | [1] Proteome-wide mapping of the Drosophila acetylome demonstrates a high degree of conservation of lysine acetylation. Weinert BT, Wagner SA, Horn H, Henriksen P, Liu WR, Olsen JV, Jensen LJ, Choudhary C. Sci Signal. 2011 Jul 26;4(183):ra48. [ PMID: 21791702] |
Functional Description | Trithorax group (TrxG) protein that has histone methyltransferase activity. Specifically trimethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation. May also trimethylate H3 'Lys-9' and H4 'Lys-20'; however the relevance of this activity is unclear in vivo. TrxG protein are generally required to maintain the transcriptionally active state of homeotic genes throughout development. Does not act as a coactivator required for transcriptional activation, but specifically prevent inappropriate Polycomb Group (PcG) silencing of homeotic genes in cells in which they must stay transcriptionally active. Binds non-coding RNAs of trithorax response element (TRE), leading to its recruitment to the corresponding TRE in chromatin. |
Sequence Annotation | DOMAIN 1339 1387 AWS.
DOMAIN 1390 1506 SET.
DOMAIN 1514 1530 Post-SET.
DOMAIN 1952 2072 BAH.
DNA_BIND 261 273 A.T hook 1.
DNA_BIND 1065 1077 A.T hook 2.
DNA_BIND 1095 1107 A.T hook 3.
ZN_FING 1857 1903 PHD-type.
MOD_RES 135 135 Phosphoserine.
MOD_RES 136 136 Phosphoserine.
MOD_RES 138 138 Phosphoserine.
MOD_RES 200 200 Phosphothreonine.
MOD_RES 201 201 Phosphothreonine.
MOD_RES 740 740 Phosphoserine.
MOD_RES 831 831 Phosphoserine.
MOD_RES 977 977 Phosphoserine. |
Keyword | Activator; Chromatin regulator; Chromosome; Complete proteome; Developmental protein; Metal-binding; Methyltransferase; Nucleus; Phosphoprotein; Reference proteome; Repeat; RNA-binding; S-adenosyl-L-methionine; Transcription; Transcription regulation; Transferase; Zinc; Zinc-finger. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 2226 AA |
Protein Sequence | MSCSQNETAA AKVLETQRAQ ESGSENEETD SITDQSSQSK SIKSATQFSV QRSDTDGLRM 60
RISAIRPTLG VVATKKPPKS RKMSTQDTES GCSEAKNRAV SKKVKVKRKK LASSSGISKS 120
DKVSKSKKSQ ISAFSSDSED DLPLKVHQQR APRVLLSAII QAAQSASKPT LDIGISSSDN 180
ELPNLVQAAI KRVESDTEDT TVEGSFRKAA KDKNLPQYQS TLLQDFMEKT QMLGQTVNAK 240
LAEEKVAKAK EETLVQTAVP RKRRGRPKKV VPTVPAPGNS GPAINESADS GVISTTSTTQ 300
STTPSPKMQN ENAVPTGSLP IASSSKPKID MAYLDKRMYA TERVLYPPPR SKRRQNNKKT 360
ACSSSNKEEL QLDPLWREID VNKKFRLRSM SVGAASGTGA STTICSKVLA AKSGYVSDYG 420
SVRHQRSSHN HNSGYKSDAS CKSRYSTKSC MSRRSRAKSC GYRSDCKESG KSGLRMRRKR 480
RASMLLKSSA DDTVEDQDIL QLAGLSLGQS SEESNEYISK PSLKSLPTTS ASKKYGEINR 540
YVTTGQYFGR GGSLSATNPD NFISKMMNQR KETPAPSKSS CKIKSRRSSA ASMCSSYVSG 600
VSRMRRRHRR KSFSHNKSLN IDSKLLTEIE IITSTFNSRC RIQDDRLTGS SGKEKLLADA 660
NKLQATLAAP SPAQQLTLNG GGPASTLSKP LKRGLKKRKL SEPLVDFAML SASASGTPNG 720
SGSSNGNTKR RHKKSQSNDS SSPDDHKLPL KKRHYLLTPG ERPPAEVAFA NGKLNAEAWA 780
AAAAAAKSTA STKSQAQFNA RSVKSALTPK KRHLLEQPTS VSGAGSSASN SPLRIVVDNN 840
SISGGKLLDI SPSSLCSLKQ QRRGGAAKQK VSAAKDLVQL QSPAGSYPPP GVFEPSVELE 900
IQIPLSKLNE SVITKAEVES PLLSALDIKE DTKKEVGQRV VETLLHKTGG NLLLKRKRKK 960
INRTGFPTVR RKKRKVSVEQ QTTAVIDEHE PEFDPDDEPL QSLRETRSSN NVNVQAAPNP 1020
PLDCERVPQA GEARETFVAR TNQKAPRLSV VALERLQRPQ TPARGRPRGR KPKNREQAEA 1080
APQPPPKSEP EIRPAKKRGR QPKQPVLEEP PPTPPPQQKK NKMEPNIRLP DGIDPNTNFS 1140
CKIRLKRRKN LEAGTQPKKE KPVQPVTVEE IPPEIPVSQE EIDAEAEAKR LDSIPTEHDP 1200
LPASESHNPG PQDYASCSES SEDKASTTSL RKLSKVKKTY LVAGLFSNHY KQSLMPPPAK 1260
VNKKPGLEEQ VGPASLLPPP PYCEKYLRRT EMDFELPYDI WWAYTNSKLP TRNVVPSWNY 1320
RKIRTNVYAE SVRPNLAGFD HPTCNCKNQG EKSCLDNCLN RMVYTECSPS NCPAGEKCRN 1380
QKIQRHAVAP GVERFMTADK GWGVRTKLPI AKGTYILEYV GEVVTEKEFK QRMASIYLND 1440
THHYCLHLDG GLVIDGQRMG SDCRFVNHSC EPNCEMQKWS VNGLSRMVLF AKRAIEEGEE 1500
LTYDYNFSLF NPSEGQPCRC NTPQCRGVIG GKSQRVKPLP AVEAKPSGEG LSGRNGRQRK 1560
QKAKKHAQRQ AGKDISSAVA VAKLQPLSEK EKKLVRQFNT FLVRNFEKIR RCKAKRASDA 1620
AATASSPALG TTNGDIPGRR PSTPSSPSLA AQISALCSPR NIKTRGLTQA VHDPELEKMA 1680
KMAVVLRDIC SAMETLKMSD LLTTVSSKKK KPIKTTLSGK LGSTAATSKV EFRSIQAQVE 1740
QGHYKTPQEF DDHMQQLFVE AKQQHGDDEG KEKALQSLKD SYEQQKIASY VQLVEILGDS 1800
ESLQSFKPKE VLSSEEEPGK IAVKKSPGAK ERDSPIVPLK VTPPPLLPIE ASPDEDVIRC 1860
ICGLYKDEGL MIQCSKCMVW QHTECTKADI DADNYQCERC EPREVDREIP LEEFTEEGHR 1920
YYLSLMRGDL QVRQGDAVYV LRDIPIKDES GKVLPTKKHT YETIGAIDYQ ECDIFRVEHL 1980
WKNELGKRFI FGHHFLRPHE TFHEPSRRFY PNEVVRVSLY EVVPIELVIG RCWVLDRTTF 2040
CKGRPMECND EDHCYICELR VDKTARFFSK AKANHPACTK SYAFRKFPEK IKISKSYAPH 2100
DVDPSLLKTR KQKTELDVGA GPTTMHKVSG RQEQHQAKMV GRKPRGISAP ADATAVHVVT 2160
PVAPNKQMLK KRKSRLENVL ITMKLKCLDA QTAQEQPIDL SYLLSGRGAR QRKTQQSSSS 2220
STANST 2226 |
Gene Ontology | GO:0005634; C:nucleus; IC:FlyBase. GO:0035327; C:transcriptionally active chromatin; IMP:FlyBase. GO:0003677; F:DNA binding; IEA:InterPro. GO:0042800; F:histone methyltransferase activity (H3-K4 specific); IDA:FlyBase. GO:0046974; F:histone methyltransferase activity (H3-K9 specific); NAS:FlyBase. GO:0042799; F:histone methyltransferase activity (H4-K20 specific); NAS:FlyBase. GO:0003727; F:single-stranded RNA binding; IDA:FlyBase. GO:0008270; F:zinc ion binding; IEA:InterPro. GO:0048096; P:chromatin-mediated maintenance of transcription; IMP:FlyBase. GO:0001700; P:embryonic development via the syncytial blastoderm; IMP:FlyBase. GO:0048477; P:oogenesis; IMP:FlyBase. GO:0018991; P:oviposition; IMP:FlyBase. GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. |
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PROSITE | |
PRINTS | |