UniProt Accession

 DDX6_HUMAN; P26196; Q5D048 

Genbank Protein ID

 Z11685; BC065007; D17532 

Genbank Nucleotide ID

 AAH65007.1; BAA04482.1 

Protein Name

 Probable ATP-dependent RNA helicase DDX6 

Protein Synonyms/Alias

 ATP-dependent RNA helicase p54; DEAD box protein 6; Oncogene RCK 

Gene Name

 DDX6 

Gene Synonyms/Alias

 HLR2; RCK 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
26	GQLRGPVKPTGGPGG	ubiquitination	[1]
67	QSMTTTIKPGDDWKK	ubiquitination	[1, 2]
73	IKPGDDWKKTLKLPP	ubiquitination	[2]
74	KPGDDWKKTLKLPPK	ubiquitination	[2]
86	PPKDLRIKTSDVTST	ubiquitination	[2, 3]
94	TSDVTSTKGNEFEDY	ubiquitination	[2, 3]
104	EFEDYCLKRELLMGI	acetylation	[3]
104	EFEDYCLKRELLMGI	ubiquitination	[2, 3]
146	RAKNGTGKSGAYLIP	ubiquitination	[1, 2, 3]
162	LERLDLKKDNIQAMV	ubiquitination	[2]
195	SKHMGGAKVMATTGG	ubiquitination	[1]
231	GRILDLIKKGVAKVD	ubiquitination	[1, 2]
232	RILDLIKKGVAKVDH	ubiquitination	[2]
352	QRVELLAKKISQLGY	ubiquitination	[2]
353	RVELLAKKISQLGYS	ubiquitination	[2]
447	YDDRFNLKSIEEQLG	ubiquitination	[1]
458	EQLGTEIKPIPSNID	ubiquitination	[1, 2, 3]
482	SEPVEDEKP******	ubiquitination	[1, 2, 3]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 In the process of mRNA degradation, may play a role in mRNA decapping. 

Sequence Annotation

 DOMAIN 127 298 Helicase ATP-binding.
 DOMAIN 308 468 Helicase C-terminal.
 NP_BIND 140 147 ATP (By similarity).
 MOTIF 96 124 Q motif.
 MOTIF 246 249 DEAD box.
 MOD_RES 36 36 Phosphothreonine.  

Keyword

 3D-structure; ATP-binding; Chromosomal rearrangement; Complete proteome; Cytoplasm; Helicase; Hydrolase; Nucleotide-binding; Phosphoprotein; Proto-oncogene; Reference proteome; RNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 483 AA 

Protein Sequence

Gene Ontology

 GO:0000932; C:cytoplasmic mRNA processing body; IDA:UniProtKB.
 GO:0010494; C:cytoplasmic stress granule; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016442; C:RNA-induced silencing complex; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0003724; F:RNA helicase activity; TAS:ProtInc.
 GO:0033962; P:cytoplasmic mRNA processing body assembly; IDA:MGI.
 GO:0043928; P:exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay; TAS:Reactome.
 GO:0010467; P:gene expression; TAS:Reactome. 

Interpro

 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR000629; RNA-helicase_DEAD-box_CS.
 IPR014014; RNA_helicase_DEAD_Q_motif. 

Pfam

 PF00270; DEAD
 PF00271; Helicase_C 

SMART

 SM00487; DEXDc
 SM00490; HELICc 

PROSITE

 PS00039; DEAD_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS51195; Q_MOTIF 

PRINTS