CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024395
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Coiled-coil domain-containing protein 66 
Protein Synonyms/Alias
  
Gene Name
 CCDC66 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
178LSLTENGKEAKSQYSubiquitination[1]
206NIMGLFKKTEMVSSVubiquitination[1]
229NEHQETSKQCEQKIAubiquitination[1]
242IAIENEWKPADIFSTubiquitination[1]
269AKKAQWRKELDEQVAacetylation[2]
325EHPFSAVKQELQRKWubiquitination[1]
356EEKIIYSKGEEHDRWubiquitination[1]
481QVEEKRRKKQLEEEQubiquitination[3]
482VEEKRRKKQLEEEQRubiquitination[3]
913DRQQAILKGLSELRQubiquitination[1, 2, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
  
Sequence Annotation
 MOD_RES 115 115 Phosphothreonine.
 MOD_RES 121 121 Phosphothreonine.
 MOD_RES 586 586 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; Coiled coil; Complete proteome; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 948 AA 
Protein Sequence
MNLGDGLKLE TELLDGKTKL ILSPYEHKSK ISVKMGNKAK IAKCPLRTKT GHILKSTQDT 60
CIGSEKLLQK KPVGSETSQA KGEKNGMTFS STKDLCKQCI DKDCLHIQKE ISPATPNMQK 120
TRNTVNTSLV GKQKPHKKHI TAENMKSSLV CLTQDQLQQI LMTVNQGNRS LSLTENGKEA 180
KSQYSLYLNS ISNQPKDENI MGLFKKTEMV SSVPAENKSV LNEHQETSKQ CEQKIAIENE 240
WKPADIFSTL GERECDRSSL EAKKAQWRKE LDEQVALKKK EKEVSEKWND PWKKSESDKI 300
IWEKHQILDQ SRETVLLEHP FSAVKQELQR KWIEELNKQI EDDRQRKIEE KIIYSKGEEH 360
DRWAMHFDSL KSYPGSQSQL FSQSTHKQPE YFCVSPDTQE LADVSSVCTP TTGSQVEPSE 420
EEHIAKPIKD VVMANSKKTN FLRSMTALLD PAQIEERDRR RQKQLEHQKA ITAQVEEKRR 480
KKQLEEEQRK KEEQEEELRL AQEREEMQKQ YEEDILKQKQ KEEIMTLKTN ELFQTMQRAQ 540
ELAQRLKQEQ RIRELAQKGH DTSRLIKNLG VDTIQMEYNA SNISNSRHDS DEISGKMNTY 600
MNSTTSKKDT GVQTDDLNIG IFTNAESHCG SLMERDITNC SSPEISAELI GQFSTKKNKQ 660
ELTQDKGASL EKENNRCNDQ CNQFTRIEKQ TKHMKKYPKR PDWNINKPPK RYIPASEKYP 720
KQLQKQREEK KVRRQMELLH LVEKNNPGHL SQNRGISPEI FHSSHQETES KLRWHLVKKE 780
EEPLNIHSFS KERSPSSPVP VVKNRTQQTQ NTLHLPLKNS SYERENLISG SNQTELSSGI 840
SESSHFIPYV RTNEIYYLDP DAPLSGPSTQ DPQYQNSQDC GQKRQLFDSD CVRDPLLNPN 900
MVKNRDRQQA ILKGLSELRQ GLLQKQKELE SSLLPLAENQ EESFGSSF 948 
Gene Ontology
  
Interpro
  
Pfam
  
SMART
  
PROSITE
  
PRINTS