| Tag | Content |
|---|
| CPLM ID | CPLM-006152 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Translation initiation factor eIF-2B subunit epsilon |
Protein Synonyms/Alias | GCD complex subunit GCD6; Guanine nucleotide exchange factor subunit GCD6; eIF-2B GDP-GTP exchange factor subunit epsilon |
Gene Name | GCD6 |
Gene Synonyms/Alias | TIF225; YDR211W; YD8142.12; YD8142B.03 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 338 | VVLAQSCKIGKCTAI | ubiquitination | [1] | | 350 | TAIGSGTKIGEGTKI | ubiquitination | [1] | | 356 | TKIGEGTKIENSVIG | ubiquitination | [1] | | 438 | KISASPLKNAGSRMY | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] |
Functional Description | Acts as a catalytic component of the translation initiation factor 2B (eIF2-B or GCD complex), which catalyzes the exchange of eukaryotic initiation factor 2 (eIF-2)-bound GDP for GTP and is regulated by phosphorylated eIF-2. It activates the synthesis of GCN4 in yeast under amino acid starvation conditions by suppressing the inhibitory effects of multiple AUG codons present in the leader of GCN4 mRNA. It may promote either repression or activation of GCN4 expression depending on amino acid availability. GCD6 and GCD7 repress GCN4 expression at the translational level by ensuring that ribosomes which have translated UORF1 will reinitiate at UORF2, -3, or -4 and thus fail to reach the GCN4 start site. |
Sequence Annotation | DOMAIN 539 710 W2.
MOD_RES 478 478 Phosphoserine.
MOD_RES 481 481 Phosphoserine.
MOD_RES 507 507 Phosphoserine.
MOD_RES 525 525 Phosphoserine.
MOD_RES 538 538 Phosphoserine.
MOD_RES 707 707 Phosphoserine. |
Keyword | 3D-structure; Complete proteome; Initiation factor; Phosphoprotein; Protein biosynthesis; Reference proteome; Translation regulation. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 712 AA |
Protein Sequence | MAGKKGQKKS GLGNHGKNSD MDVEDRLQAV VLTDSYETRF MPLTAVKPRC LLPLANVPLI 60
EYTLEFLAKA GVHEVFLICS SHANQINDYI ENSKWNLPWS PFKITTIMSP EARCTGDVMR 120
DLDNRGIITG DFILVSGDVL TNIDFSKMLE FHKKMHLQDK DHISTMCLSK ASTYPKTRTI 180
EPAAFVLDKS TSRCIYYQDL PLPSSREKTS IQIDPELLDN VDEFVIRNDL IDCRIDICTS 240
HVPLIFQENF DYQSLRTDFV KGVISSDILG KHIYAYLTDE YAVRVESWQT YDTISQDFLG 300
RWCYPLVLDS NIQDDQTYSY ESRHIYKEKD VVLAQSCKIG KCTAIGSGTK IGEGTKIENS 360
VIGRNCQIGE NIRIKNSFIW DDCIIGNNSI IDHSLIASNA TLGSNVRLND GCIIGFNVKI 420
DDNMDLDRNT KISASPLKNA GSRMYDNESN EQFDQDLDDQ TLAVSIVGDK GVGYIYESEV 480
SDDEDSSTEA CKEINTLSNQ LDELYLSDDS ISSATKKTKK RRTMSVNSIY TDREEIDSEF 540
EDEDFEKEGI ATVERAMENN HDLDTALLEL NTLRMSMNVT YHEVRIATIT ALLRRVYHFI 600
ATQTLGPKDA VVKVFNQWGL LFKRQAFDEE EYIDLMNIIM EKIVEQSFDK PDLILFSALV 660
SLYDNDIIEE DVIYKWWDNV STDPRYDEVK KLTVKWVEWL QNADEESSSE EE 712 |
Gene Ontology | GO:0005851; C:eukaryotic translation initiation factor 2B complex; IDA:SGD. GO:0032045; C:guanyl-nucleotide exchange factor complex; IDA:SGD. GO:0005085; F:guanyl-nucleotide exchange factor activity; IDA:SGD. GO:0016779; F:nucleotidyltransferase activity; IEA:InterPro. GO:0003743; F:translation initiation factor activity; IGI:SGD. GO:0006446; P:regulation of translational initiation; IDA:SGD. GO:0016070; P:RNA metabolic process; IEA:InterPro. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |