CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023721
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Zinc finger protein 281 
Protein Synonyms/Alias
 GC-box-binding zinc finger protein 1; Transcription factor ZBP-99; Zinc finger DNA-binding protein 99 
Gene Name
 ZNF281 
Gene Synonyms/Alias
 GZP1; ZBP99 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
102APDMTFKKEPAASAAubiquitination[1]
315EKIHSREKPFGCDQCubiquitination[1]
329CSMKFIQKYHMERHKubiquitination[1]
343KRTHSGEKPYKCDTCubiquitination[1]
346HSGEKPYKCDTCQQYubiquitination[1]
373TCGEVIVKGATSAEPubiquitination[1]
419AIENKEQKTGKTNESubiquitination[1]
477GSRKNTDKNYLNFVSubiquitination[1]
498GQKSLSGKPSGSLGIubiquitination[1]
539DDAMQFSKKRRYLPTubiquitination[1, 2, 3]
599EIKSCHDKSGIPDEVubiquitination[1]
617ILDQYSNKSESQKEDubiquitination[1, 3]
622SNKSESQKEDPFNIAubiquitination[1, 2, 3]
661TQTPSNDKASMLQEYubiquitination[1, 2]
670SMLQEYSKYLQQAFEubiquitination[1, 2, 3]
787AFQSSSQKLTSQKEQubiquitination[1, 2, 3]
795LTSQKEQKNLESSTGubiquitination[2]
818ASQIDPQKDIEPRTTubiquitination[1, 3]
840QAFGSQFKSGSRVPMubiquitination[1, 2, 3, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 
Functional Description
 Transcription repressor that plays a role in regulation of embryonic stem cells (ESCs) differentiation. Required for ESCs differentiation and acts by mediating autorepression of NANOG in ESCs: binds to the NANOG promoter and promotes association of NANOG protein to its own promoter and recruits the NuRD complex, which deacetylates histones. Not required for establishement and maintenance of ESCs (By similarity). Represses the transcription of a number of genes including GAST, ODC1 and VIM. Binds to the G- rich box in the enhancer region of these genes. 
Sequence Annotation
 ZN_FING 261 283 C2H2-type 1.
 ZN_FING 289 311 C2H2-type 2.
 ZN_FING 317 339 C2H2-type 3.
 ZN_FING 345 367 C2H2-type 4; atypical.
 MOD_RES 395 395 Phosphoserine.
 MOD_RES 484 484 Phosphoserine.
 MOD_RES 785 785 Phosphoserine.
 MOD_RES 807 807 Phosphoserine.  
Keyword
 Complete proteome; Differentiation; DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 895 AA 
Protein Sequence
MKIGSGFLSG GGGTGSSGGS GSGGGGSGGG GGGGSSGRRA EMEPTFPQGM VMFNHRLPPV 60
TSFTRPAGSA APPPQCVLSS STSAAPAAEP PPPPAPDMTF KKEPAASAAA FPSQRTSWGF 120
LQSLVSIKQE KPADPEEQQS HHHHHHHHYG GLFAGAEERS PGLGGGEGGS HGVIQDLSIL 180
HQHVQQQPAQ HHRDVLLSSS SRTDDHHGTE EPKQDTNVKK AKRPKPESQG IKAKRKPSAS 240
SKPSLVGDGE GAILSPSQKP HICDHCSAAF RSSYHLRRHV LIHTGERPFQ CSQCSMGFIQ 300
KYLLQRHEKI HSREKPFGCD QCSMKFIQKY HMERHKRTHS GEKPYKCDTC QQYFSRTDRL 360
LKHRRTCGEV IVKGATSAEP GSSNHTNMGN LAVLSQGNTS SSRRKTKSKS IAIENKEQKT 420
GKTNESQISN NINMQSYSVE MPTVSSSGGI IGTGIDELQK RVPKLIFKKG SRKNTDKNYL 480
NFVSPLPDIV GQKSLSGKPS GSLGIVSNNS VETIGLLQST SGKQGQISSN YDDAMQFSKK 540
RRYLPTASSN SAFSINVGHM VSQQSVIQSA GVSVLDNEAP LSLIDSSALN AEIKSCHDKS 600
GIPDEVLQSI LDQYSNKSES QKEDPFNIAE PRVDLHTSGE HSELVQEENL SPGTQTPSND 660
KASMLQEYSK YLQQAFEKST NASFTLGHGF QFVSLSSPLH NHTLFPEKQI YTTSPLECGF 720
GQSVTSVLPS SLPKPPFGML FGSQPGLYLS ALDATHQQLT PSQELDDLID SQKNLETSSA 780
FQSSSQKLTS QKEQKNLESS TGFQIPSQEL ASQIDPQKDI EPRTTYQIEN FAQAFGSQFK 840
SGSRVPMTFI TNSNGEVDHR VRTSVSDFSG YTNMMSDVSE PCSTRVKTPT SQSYR 895 
Gene Ontology
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0001047; F:core promoter binding; ISS:UniProtKB.
 GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:UniProtKB.
 GO:0003714; F:transcription corepressor activity; ISS:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0010172; P:embryonic body morphogenesis; ISS:UniProtKB.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0048863; P:stem cell differentiation; ISS:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
  
SMART
 SM00355; ZnF_C2H2 
PROSITE
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS