CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037550
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Nuclear RNA export factor 1 
Protein Synonyms/Alias
  
Gene Name
 NXF1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
160TSQDGTSKNWFKITIubiquitination[1, 2]
164GTSKNWFKITIPYGRubiquitination[1, 2]
216ASTASALKAVNYKILubiquitination[3]
248HTILNELKPEQVEQLubiquitination[1]
273SQQALDLKGLRSDPDubiquitination[1, 2, 3, 4]
332DMSSIVQKAPNLKILubiquitination[1, 4]
337VQKAPNLKILNLSGNubiquitination[1, 4]
347NLSGNELKSERELDKubiquitination[1, 2]
390AIRERFPKLLRLDGHubiquitination[1]
480SSLAEYFKDSRNVKKubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Leucine-rich repeat; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 603 AA 
Protein Sequence
MADEGKSYSV STSFFKSPTQ FSSNFSLMLP SHREGSFLWI GSGVCMEMAS VVKHDDERVN 60
FPQRKKKGRG PFRWKYGEGN RRSGRGGSGI RSSRLEEDDG DVAMSDAQDG PRVRYNPYTT 120
RPNRRGDTWH DRDRIHVTVR RDRAPPERGG AGTSQDGTSK NWFKITIPYG RKYDKAWLLS 180
MIQSKCSVPF TPIEFHYENT RAQFFVEDAS TASALKAVNY KILDRENRRI SIIINSSAPP 240
HTILNELKPE QVEQLKLIMS KRYDGSQQAL DLKGLRSDPD LVAQNIDVVL NRRSCMAATL 300
RIIEENIPEL LSLNLSNNRL YRLDDMSSIV QKAPNLKILN LSGNELKSER ELDKIKGLKL 360
EELWLDGNSL CDTFRDQSTY ISAIRERFPK LLRLDGHELP PPIAFDVEAP TTLPPCKGSY 420
FGTENLKSLV LHFLQQYYAI YDSGDRQGLL DAYHDGACCS LSIPFIPQNP ARSSLAEYFK 480
DSRNVKKLKD PTLRFRLLKH TRLNVVAFLN ELPKTQHDVN SFVVDISAQT STLLCFSVNG 540
VFKEVDGKSR DSLRAFTRTF IAVPASNSGL CIVNDELFVR NASSEEIQRA FAMPAPTPSS 600
SPV 603 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0005634; C:nucleus; IEA:InterPro.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0006406; P:mRNA export from nucleus; IEA:InterPro. 
Interpro
 IPR001611; Leu-rich_rpt.
 IPR002075; NTF2.
 IPR018222; Nuclear_transport_factor_2_euk.
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR015245; Tap_RNA-bd.
 IPR003603; U2A'_phosphoprotein32A_C. 
Pfam
 PF00560; LRR_1
 PF02136; NTF2
 PF09162; Tap-RNA_bind 
SMART
 SM00446; LRRcap 
PROSITE
 PS51450; LRR
 PS50177; NTF2_DOMAIN 
PRINTS