CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016595
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Isoleucine--tRNA ligase, cytoplasmic 
Protein Synonyms/Alias
 Isoleucyl-tRNA synthetase; IRS; IleRS 
Gene Name
 Iars 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
110KGPEDVAKMGIAEYNubiquitination[1]
177GLVYRGVKVMPFSTAubiquitination[1]
254IKDVARGKLFILTEAacetylation[2]
268ARLSALYKQESDYEIacetylation[2]
288GASLKGKKYKPLFDYacetylation[2]
290SLKGKKYKPLFDYFIubiquitination[1]
375QYVKDADKNIIRMLKacetylation[3]
410SDTPLIYKSVPSWFVubiquitination[1]
600ILASDGQKMSKRKKNubiquitination[1]
796DPASLRDKDTLSIHYacetylation[3]
796DPASLRDKDTLSIHYubiquitination[1]
844DRKTIPIKYPLKEIVacetylation[4]
1042ATIKAPLKPYPVPTSacetylation[3, 4]
1125VTSVFGVKNAKLSVFubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 
Functional Description
  
Sequence Annotation
 MOTIF 48 58 "HIGH" region.
 MOTIF 600 604 "KMSKS" region.
 BINDING 603 603 ATP (By similarity).
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 1049 1049 Phosphoserine (By similarity).  
Keyword
 Acetylation; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding; Phosphoprotein; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1262 AA 
Protein Sequence
MVQQVPENIS FPAEEEKILE FWSKHNCFQE CLKQSKLRPK FTFYDGPPFA TGLPHYGHIL 60
AGTIKDIVTR YAHQSGFHVD RRFGWDCHGL PVEYEIDKTL GIKGPEDVAK MGIAEYNKQC 120
RAIVMRYSAE WKSTVTRLGR WIDFDNDYKT LYPQFMESVW WVFKQLYDKG LVYRGVKVMP 180
FSTACGTPLS NFESNQNYKD VQDPSVFVTF PLEEDENTSL VAWTTTPWTL PSNLALCVNP 240
EIQYVKIKDV ARGKLFILTE ARLSALYKQE SDYEILERFP GASLKGKKYK PLFDYFIKCK 300
ENGAFTVLVD HYVKDEEGTG VVHQAPYFGA DDHRVCMDFN IIQKDSVPVC PVDASGCFTE 360
EVTHFVGQYV KDADKNIIRM LKEQGRLLAA GTFTHSYPFC WRSDTPLIYK SVPSWFVRVE 420
PMVDQLLKNN DLCYWVPEFV REKRFGNWLK EARDWAISRN RYWGTPIPLW VSEDLEEVVC 480
IGSVAELEEL SGTKISDLHR ESIDHLTIPS RCGKAPLRRV SEVFDCWFES GSMPYAQVHY 540
PFESKREFED AFPADFIAEG IDQTRGWFYT LLVLATALFG QPPFKNVIVN GLILASDGQK 600
MSKRKKNYPD PVSIIDKYGA DALRLYLINS PVVRAENLRF KEEGVRDVLK DVLLPWYNAY 660
RFFIQNVFRL HKEEEVKFLY NEHTVRESPN ITDRWVLSFM QSLLGFFETE MAAYRLYTVV 720
PRLVKFVDIL TNWYVRMNRR RLKGESGVED CVMALETLFS VLLSLCRLMA PYTPFLTELM 780
YQNLKLLIDP ASLRDKDTLS IHYLMLPRVR EELIDKKTEN AVSRMQSVIE LGRVIRDRKT 840
IPIKYPLKEI VVIHQDPEAL EDIRSLEKYI IEELNVRKVT LSTDKNKYGI RLRAEPDHMV 900
LGKRLKGAFK AVMMAIKRLS NEELERFQKS GSIVVEGHEL HEEDIRLMYT FDQATGGTAQ 960
FEAHSDAQAL VLLDVTPDQS MVDEGMAREV INRIQKLRKK CNLVPTDEIT VYYNAKSEGR 1020
YLNNVIESHT DFIFATIKAP LKPYPVPTSD NILIQEQTQL KGSELEITLT KGSCVPGPAC 1080
AYVNLNICAN GTEQGGVLLL ENPKGDNQLN LVKLKTVVTS VFGVKNAKLS VFHGETEIQN 1140
QTDLLSLSGR TLCVTAGASP SPISSPSTLL CQYLNLQLLN AEPQECLTGT VGTLLLENPL 1200
GQNGLTHQGL VHEAAKVFGL RSRRLRLFLN ETQTQEITED IPMKTLNMKT VYVSVLPTTA 1260
DG 1262 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004822; F:isoleucine-tRNA ligase activity; IEA:EC.
 GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
 GO:0006450; P:regulation of translational fidelity; IEA:GOC. 
Interpro
 IPR001412; aa-tRNA-synth_I_CS.
 IPR002300; aa-tRNA-synth_Ia.
 IPR002301; Ile-tRNA-ligase.
 IPR023586; Ile-tRNA-ligase_type2.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR009080; tRNAsynth_1a_anticodon-bd.
 IPR013155; V/L/I-tRNA-synth_anticodon-bd.
 IPR009008; Val/Leu/Ile-tRNA-synth_edit. 
Pfam
 PF08264; Anticodon_1
 PF00133; tRNA-synt_1 
SMART
  
PROSITE
 PS00178; AA_TRNA_LIGASE_I 
PRINTS
 PR00984; TRNASYNTHILE.