UniProt Accession

 SYHC_MOUSE; Q61035; Q3TKU3 

Genbank Protein ID

 U39473; AK146922; AK166826; AK166892 

Genbank Nucleotide ID

 AAC52914.1; BAE27533.1; BAE39050.1; BAE39097.1 

Protein Name

 Histidine--tRNA ligase, cytoplasmic 

Protein Synonyms/Alias

 Histidyl-tRNA synthetase; HisRS 

Gene Name

 Hars 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
37	QIEEEVTKLLKLKAQ	ubiquitination	[1]
106	LKETLTGKYGEDSKL	acetylation	[2]
112	GKYGEDSKLIYDLKD	acetylation	[3]
143	ARYLAMNKLTNIKRY	acetylation	[2]
240	TICSSVDKLDKVSWE	acetylation	[2]
293	DPKLSQNKQAVEGLG	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 

Functional Description

  

Sequence Annotation

 DOMAIN 3 59 WHEP-TRS.
 MOD_RES 2 2 N-acetylalanine (By similarity).  

Keyword

 Acetylation; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 509 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004821; F:histidine-tRNA ligase activity; IEA:EC.
 GO:0006427; P:histidyl-tRNA aminoacylation; IEA:InterPro. 

Interpro

 IPR006195; aa-tRNA-synth_II.
 IPR004154; Anticodon-bd.
 IPR015807; His-tRNA-ligase.
 IPR004516; HisRS/HisZ.
 IPR009068; S15_NS1_RNA-bd.
 IPR000738; WHEP-TRS. 

Pfam

 PF03129; HGTP_anticodon
 PF00458; WHEP-TRS 

SMART

 SM00991; WHEP-TRS 

PROSITE

 PS50862; AA_TRNA_LIGASE_II
 PS00762; WHEP_TRS_1
 PS51185; WHEP_TRS_2 

PRINTS