CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001271
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 AP-1 complex subunit gamma-like 2 
Protein Synonyms/Alias
 Gamma2-adaptin; G2ad 
Gene Name
 AP1G2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
7*MVVPSLKLQDLIEEubiquitination[1, 2]
19IEEIRGAKTQAQEREubiquitination[2]
30QEREVIQKECAHIRAubiquitination[2]
145DLAPEVEKLLLQPSPubiquitination[1, 2, 3, 4, 5]
156QPSPYVRKKAILTAVubiquitination[2]
157PSPYVRKKAILTAVHubiquitination[2]
168TAVHMIRKVPELSSVubiquitination[2]
215AALRHFRKVVPQLVHubiquitination[2, 4]
533YALTALMKLSTRLCGubiquitination[2]
584MRAAILEKMPLVERDubiquitination[2, 4, 6]
752FRILNPNKAPLRLKLubiquitination[2]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 May function in protein sorting in late endosomes or multivesucular bodies (MVBs). Involved in MVB-assisted maturation of hepatitis B virus (HBV). 
Sequence Annotation
 DOMAIN 665 780 GAE.
 REGION 369 379 Essential for ubiquitin-binding.  
Keyword
 3D-structure; Complete proteome; Cytoplasmic vesicle; Endosome; Golgi apparatus; Host-virus interaction; Membrane; Polymorphism; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 785 AA 
Protein Sequence
MVVPSLKLQD LIEEIRGAKT QAQEREVIQK ECAHIRASFR DGDPVHRHRQ LAKLLYVHML 60
GYPAHFGQME CLKLIASSRF TDKRVGYLGA MLLLDERHDA HLLITNSIKN DLSQGIQPVQ 120
GLALCTLSTM GSAEMCRDLA PEVEKLLLQP SPYVRKKAIL TAVHMIRKVP ELSSVFLPPC 180
AQLLHERHHG ILLGTITLIT ELCERSPAAL RHFRKVVPQL VHILRTLVTM GYSTEHSISG 240
VSDPFLQVQI LRLLRILGRN HEESSETMND LLAQVATNTD TSRNAGNAVL FETVLTIMDI 300
RSAAGLRVLA VNILGRFLLN SDRNIRYVAL TSLLRLVQSD HSAVQRHRPT VVECLRETDA 360
SLSRRALELS LALVNSSNVR AMMQELQAFL ESCPPDLRAD CASGILLAAE RFAPTKRWHI 420
DTILHVLTTA GTHVRDDAVA NLTQLIGGAQ ELHAYSVRRL YNALAEDISQ QPLVQVAAWC 480
IGEYGDLLLA GNCEEIEPLQ VDEEEVLALL EKVLQSHMSL PATRGYALTA LMKLSTRLCG 540
DNNRIRQVVS IYGSCLDVEL QQRAVEYDTL FRKYDHMRAA ILEKMPLVER DGPQADEEAK 600
ESKEAAQLSE AAPVPTEPQA SQLLDLLDLL DGASGDVQHP PHLDPSPGGA LVHLLDLPCV 660
PPPPAPIPDL KVFEREGVQL NLSFIRPPEN PALLLITITA TNFSEGDVTH FICQAAVPKS 720
LQLQLQAPSG NTVPARGGLP ITQLFRILNP NKAPLRLKLR LTYDHFHQSV QEIFEVNNLP 780
VESWQ 785 
Gene Ontology
 GO:0030121; C:AP-1 adaptor complex; TAS:UniProtKB.
 GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
 GO:0008565; F:protein transporter activity; IEA:InterPro.
 GO:0006886; P:intracellular protein transport; IEA:InterPro.
 GO:0016192; P:vesicle-mediated transport; NAS:UniProtKB.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 
Interpro
 IPR017107; AP1_complex_gsu.
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR002553; Clathrin/coatomer_adapt-like_N.
 IPR008152; Clathrin_a/b/g-adaptin_app_Ig.
 IPR008153; Clathrin_g-adaptin_app.
 IPR013041; Coatomer/clathrin_app_Ig-like. 
Pfam
 PF01602; Adaptin_N
 PF02883; Alpha_adaptinC2 
SMART
 SM00809; Alpha_adaptinC2 
PROSITE
 PS50180; GAE 
PRINTS