CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002976
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Oligoribonuclease 
Protein Synonyms/Alias
  
Gene Name
 orn 
Gene Synonyms/Alias
 o204a; yjeR; b4162; JW5740 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
94LATLEFLKQWVPAGKacetylation[1]
101KQWVPAGKSPICGNSacetylation[1]
138YLDVSTLKELARRWKacetylation[1]
145KELARRWKPEILDGFacetylation[1]
154EILDGFTKQGTHQAMacetylation[1]
180YYREHFIKL******acetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 3'-to-5' exoribonuclease specific for small oligoribonucleotides 2 to 5 nucleotides in length, as well as small (2 to 5 nucleotides) ssDNA oligomers. Probably responsible for the final step in mRNA degradation. 
Sequence Annotation
 DOMAIN 8 171 Exonuclease.
 ACT_SITE 129 129 Potential.
 METAL 12 12 Zinc.
 METAL 14 14 Zinc.
 METAL 163 163 Zinc.  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Exonuclease; Hydrolase; Metal-binding; Nuclease; Reference proteome; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 181 AA 
Protein Sequence
MSANENNLIW IDLEMTGLDP ERDRIIEIAT LVTDANLNIL AEGPTIAVHQ SDEQLALMDD 60
WNVRTHTASG LVERVKASTM GDREAELATL EFLKQWVPAG KSPICGNSIG QDRRFLFKYM 120
PELEAYFHYR YLDVSTLKEL ARRWKPEILD GFTKQGTHQA MDDIRESVAE LAYYREHFIK 180
L 181 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0000175; F:3'-5'-exoribonuclease activity; IDA:EcoCyc.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0034611; F:oligoribonucleotidase activity; IDA:EcoCyc.
 GO:0008310; F:single-stranded DNA specific 3'-5' exodeoxyribonuclease activity; IDA:EcoCyc.
 GO:0006401; P:RNA catabolic process; IMP:EcoCyc. 
Interpro
 IPR006055; Exonuclease.
 IPR013520; Exonuclease_RNaseT/DNA_pol3.
 IPR022894; Oligoribonuclease.
 IPR012337; RNaseH-like_dom. 
Pfam
 PF00929; RNase_T 
SMART
 SM00479; EXOIII 
PROSITE
  
PRINTS