UniProt Accession

 MAFG_HUMAN; O15525 

Genbank Protein ID

 Y11514; U84249; AF059195; BC012327 

Genbank Nucleotide ID

 CAA72284.1; AAC51737.1; AAC14427.1; AAH12327.1 

Protein Name

 Transcription factor MafG 

Protein Synonyms/Alias

 V-maf musculoaponeurotic fibrosarcoma oncogene homolog G; hMAF 

Gene Name

 MAFG 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
14	GNKALKVKREPGENG	sumoylation	[1]
46	QHLRGLSKEEIVQLK	ubiquitination	[2]
53	KEEIVQLKQRRRTLK	acetylation	[3]
60	KQRRRTLKNRGYAAS	acetylation	[3]
71	YAASCRVKRVTQKEE	acetylation	[3]
76	RVKRVTQKEELEKQK	acetylation	[3]
83	KEELEKQKAELQQEV	ubiquitination	[2]
110	ELDALRSKYEALQTF	ubiquitination	[2, 4, 5, 6, 7, 8, 9]

Reference

 [1] MafG sumoylation is required for active transcriptional repression.
 Motohashi H, Katsuoka F, Miyoshi C, Uchimura Y, Saitoh H, Francastel C, Engel JD, Yamamoto M.
 Mol Cell Biol. 2006 Jun;26(12):4652-63. [PMID: 16738329]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Stimulation of NF-E2 DNA binding by CREB-binding protein (CBP)-mediated acetylation.
 Hung HL, Kim AY, Hong W, Rakowski C, Blobel GA.
 J Biol Chem. 2001 Apr 6;276(14):10715-21. [PMID: 11154691]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [9] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. Transcription factor, component of erythroid-specific transcription factor NF- E2. Activates globin gene expression when associated with NF-E2. May be involved in signal transduction of extracellular H(+) (By similarity). 

Sequence Annotation

 DOMAIN 51 114 bZIP.
 REGION 53 76 Basic motif (By similarity).
 REGION 79 93 Leucine-zipper (By similarity).
 MOD_RES 53 53 N6-acetyllysine (Probable).
 MOD_RES 60 60 N6-acetyllysine (Probable).
 MOD_RES 71 71 N6-acetyllysine (Probable).
 MOD_RES 76 76 N6-acetyllysine (Probable).
 MOD_RES 124 124 Phosphoserine.
 CROSSLNK 14 14 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; Complete proteome; DNA-binding; Isopeptide bond; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 162 AA 

Protein Sequence

Gene Ontology

 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; NAS:ProtInc.
 GO:0030534; P:adult behavior; IEA:Compara.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0042127; P:regulation of cell proliferation; IEA:Compara.
 GO:0030641; P:regulation of cellular pH; IEA:Compara.
 GO:0045604; P:regulation of epidermal cell differentiation; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR004827; bZIP.
 IPR004826; bZIP_Maf.
 IPR008917; Euk_TF_DNA-bd.
 IPR024874; Transciption_factor_Maf. 

Pfam

 PF03131; bZIP_Maf 

SMART

 SM00338; BRLZ 

PROSITE

 PS50217; BZIP
 PS00036; BZIP_BASIC 

PRINTS