UniProt Accession

 CNOT2_HUMAN; Q9NZN8; Q9H3E0; Q9NSX5; Q9NWR6; Q9P028 

Genbank Protein ID

 AF180473; AF113226; AF161480; AK000662; AL137674; BX641116; BC002597; BC011826; AF044215 

Genbank Nucleotide ID

 AAF29827.1; AAG39297.1; AAF29095.1; BAA91313.1; CAB70869.1; CAE46054.1; AAH02597.1; AAH11826.1; AAQ13426.1 

Protein Name

 CCR4-NOT transcription complex subunit 2 

Protein Synonyms/Alias

 CCR4-associated factor 2 

Gene Name

 CNOT2 

Gene Synonyms/Alias

 CDC36; NOT2; HSPC131; MSTP046 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
490	PGMEPTMKTNTYERG	ubiquitination	[1, 2]
520	EFHLEYDKLEERPHL	ubiquitination	[2]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Required for the CCR4-NOT complex structural integrity. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may specificly involve the N-Cor repressor complex containing HDAC3, NCOR1 and NCOR2. Involved in the maintenance of emryonic stem (ES) cell identity. 

Sequence Annotation

 REGION 437 540 Repressor domain.
 MOD_RES 93 93 Phosphothreonine.
 MOD_RES 126 126 Phosphoserine.
 MOD_RES 165 165 Phosphoserine.  

Keyword

 Alternative splicing; Complete proteome; Cytoplasm; Developmental protein; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repressor; RNA-mediated gene silencing; Transcription; Transcription regulation; Translation regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 540 AA 

Protein Sequence

Gene Ontology

 GO:0030014; C:CCR4-NOT complex; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; NAS:UniProtKB.
 GO:0001104; F:RNA polymerase II transcription cofactor activity; TAS:UniProtKB.
 GO:0001226; F:RNA polymerase II transcription corepressor binding; IDA:UniProtKB.
 GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
 GO:0007275; P:multicellular organismal development; IEA:UniProtKB-KW.
 GO:0033147; P:negative regulation of intracellular estrogen receptor signaling pathway; IMP:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; TAS:Reactome.
 GO:0010606; P:positive regulation of cytoplasmic mRNA processing body assembly; IMP:UniProtKB.
 GO:2000036; P:regulation of stem cell maintenance; IMP:UniProtKB.
 GO:0006417; P:regulation of translation; IEA:UniProtKB-KW. 

Interpro

 IPR007282; NOT. 

Pfam

 PF04153; NOT2_3_5 

SMART

  

PROSITE

  

PRINTS