UniProt Accession

 HIS5_YEAST; P33734; D6VQP4 

Genbank Protein ID

 X69815; Z36117; BK006936 

Genbank Nucleotide ID

 CAA49469.1; CAA85211.1; DAA07364.1 

Protein Name

 Imidazole glycerol phosphate synthase hisHF 

Protein Synonyms/Alias

 IGP synthase; IGPS; ImGP synthase; Glutamine amidotransferase; Cyclase 

Gene Name

 HIS7 

Gene Synonyms/Alias

 YBR248C; YBR1640 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
172	GWKIAKAKYGSEEFI	acetylation	[1]
199	FHPEKSGKAGLNVIE	ubiquitination	[2]
258	QGDLVVTKGDQYDVR	acetylation	[1]
258	QGDLVVTKGDQYDVR	ubiquitination	[2]
278	KGVRNLGKPVQLAQK	acetylation	[1]
334	LTVGGGIKDIVDVDG	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The glutamine amidotransferase domain provides the ammonia necessary to the cyclase domain to produce IGP and AICAR from PRFAR. 

Sequence Annotation

 DOMAIN 3 218 Glutamine amidotransferase type-1.
 REGION 236 552 Cyclase.
 REGION 364 365 PRFAR binding.
 REGION 402 404 PRFAR binding.
 REGION 474 475 PRFAR binding.
 REGION 500 501 PRFAR binding.
 REGION 523 524 PRFAR binding.
 ACT_SITE 83 83 For GATase activity (By similarity).
 ACT_SITE 193 193 For GATase activity (By similarity).
 ACT_SITE 195 195 For GATase activity (By similarity).
 ACT_SITE 245 245 Potential.
 ACT_SITE 404 404 Potential.
 BINDING 83 83 Glutamine (covalent).
 BINDING 332 332 Substrate; via amide nitrogen.
 BINDING 469 469 Substrate.  

Keyword

 3D-structure; Amino-acid biosynthesis; Complete proteome; Glutamine amidotransferase; Histidine biosynthesis; Lyase; Multifunctional enzyme; Reference proteome; Stress response; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 552 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0005622; C:intracellular; TAS:SGD.
 GO:0000107; F:imidazoleglycerol-phosphate synthase activity; IDA:SGD.
 GO:0016833; F:oxo-acid-lyase activity; IEA:InterPro.
 GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
 GO:0000105; P:histidine biosynthetic process; IMP:SGD.
 GO:0006950; P:response to stress; IEA:UniProtKB-KW. 

Interpro

 IPR013785; Aldolase_TIM.
 IPR017926; GATASE.
 IPR006062; His_biosynth.
 IPR004651; HisF.
 IPR014640; IGPS_HisHF.
 IPR010139; Imidazole-glycPsynth_HisH.
 IPR011060; RibuloseP-bd_barrel. 

Pfam

 PF00117; GATase
 PF00977; His_biosynth 

SMART

  

PROSITE

 PS51273; GATASE_TYPE_1 

PRINTS