CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-044150
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Putative ATP-dependent RNA helicase DHX33 
Protein Synonyms/Alias
  
Gene Name
 DHX33 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
78TSEDTRIKFLTDGMLubiquitination[1]
97ISDSLLRKYSCVILDubiquitination[1]
223KTCRDIAKHLPDGCPubiquitination[1]
255RVFQGAPKGYRKVIIubiquitination[1, 2]
286VVDTGMVKAKKYNPDubiquitination[1, 3]
288DTGMVKAKKYNPDSGubiquitination[1]
289TGMVKAKKYNPDSGLubiquitination[1]
306LAVQRVSKTQAWQRTubiquitination[1]
335YTEDEFEKFDKMTVPubiquitination[1, 3, 4]
490GGNKDWCKENFVNSKubiquitination[1, 2]
497KENFVNSKNMTLVAEubiquitination[3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Hydrolase; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 617 AA 
Protein Sequence
XRAAAAEEEE EAGGSSRPWP SPRPVPTLKL WSCSAGVCPS SKRGGNCWPS SETWTTRSSS 60
VGYTVRFDDV TSEDTRIKFL TDGMLLREAI SDSLLRKYSC VILDEAHERT IHTDVLFGVV 120
KAAQKRRKEL GKLPLKVIVM SATMDVDLFS QYFNGAPVLY LEGRQHPIQV FYTKQPQNDY 180
LHAALVSVFQ IHQEAPSSQD ILVFLTGQEE IEAMSKTCRD IAKHLPDGCP AMLVLPLYAS 240
LPYAQQLRVF QGAPKGYRKV IISTNIAETS ITITGIKYVV DTGMVKAKKY NPDSGLEVLA 300
VQRVSKTQAW QRTGRAGRED SGICYRLYTE DEFEKFDKMT VPEIQRCNLA SVMLQLLAMK 360
VPNVLTFDFM SKPSPDHIQA AIAQLDLLGA LEHKDDQLTL TPMGRKMAAF PLEPKFAKTI 420
LMSPKFHCTE EILTIVSLLS VDSVLHNPPS RREEVQGVRK KFISSEGDHM TLLNIYRTFK 480
NLGGNKDWCK ENFVNSKNMT LVAEVRAQLR DICLKMSMPI ASSRGDVESV RRCLAHSLFM 540
STAELQPDGT YATTDTHQPV AIHPSSVLFH CKPACVVYTE LLYTNKCYMR DLCVIDAQWL 600
YEAAPEYFRR KLRTARN 617 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003676; F:nucleic acid binding; IEA:InterPro. 
Interpro
 IPR002464; DNA/RNA_helicase_DEAH_CS.
 IPR011709; DUF1605.
 IPR007502; Helicase-assoc_dom.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase. 
Pfam
 PF04408; HA2
 PF00271; Helicase_C
 PF07717; OB_NTP_bind 
SMART
 SM00847; HA2
 SM00490; HELICc 
PROSITE
 PS00690; DEAH_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER 
PRINTS