UniProt Accession

 DHB8_MOUSE; P50171; Q5M9K0; Q60958; Q60959; Q9Z1W2 

Genbank Protein ID

 U34072; U34072; AF100956; BC086927 

Genbank Nucleotide ID

 AAC53573.1; AAC53574.1; AAC69902.1; AAH86927.1 

Protein Name

 Estradiol 17-beta-dehydrogenase 8 

Protein Synonyms/Alias

 17-beta-hydroxysteroid dehydrogenase 8; 17-beta-HSD 8; 3-oxoacyl-[acyl-carrier-protein] reductase; Protein Ke6; Ke-6; Testosterone 17-beta-dehydrogenase 8 

Gene Name

 Hsd17b8 

Gene Synonyms/Alias

 H2-Ke6; Hke6 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
66	EDGAPRGKHAAFQAD	acetylation	[1]
171	QTNYASSKAGVIGLT	acetylation	[1]

Reference

 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123] 

Functional Description

 NAD-dependent 17-beta-hydroxysteroid dehydrogenase with highest activity towards estradiol. Has very low activity towards testosterone (By similarity). The heteroteramer with CBR4 has NADH-dependent 3-ketoacyl-acyl carrier protein reductase activity. May play a role in biosynthesis of fatty acids in mitochondria (By similarity). 

Sequence Annotation

 NP_BIND 13 21 NAD (By similarity).
 NP_BIND 40 41 NAD (By similarity).
 NP_BIND 72 74 NAD (By similarity).
 NP_BIND 167 171 NAD (By similarity).
 NP_BIND 200 202 NAD (By similarity).
 ACT_SITE 167 167 Proton acceptor (By similarity).
 BINDING 154 154 Substrate (By similarity).
 MOD_RES 66 66 N6-acetyllysine.  

Keyword

 Acetylation; Alternative splicing; Complete proteome; Fatty acid biosynthesis; Fatty acid metabolism; Lipid biosynthesis; Lipid metabolism; Mitochondrion; NAD; Oxidoreductase; Reference proteome; Steroid biosynthesis. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 259 AA 

Protein Sequence

Gene Ontology

 GO:0005740; C:mitochondrial envelope; IDA:MGI.
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IDA:MGI.
 GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IEA:Compara.
 GO:0004303; F:estradiol 17-beta-dehydrogenase activity; ISS:UniProtKB.
 GO:0050327; F:testosterone 17-beta-dehydrogenase (NAD+) activity; IDA:MGI.
 GO:0047035; F:testosterone dehydrogenase (NAD+) activity; IEA:EC.
 GO:0008209; P:androgen metabolic process; IDA:MGI.
 GO:0006703; P:estrogen biosynthetic process; ISS:UniProtKB.
 GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniPathway. 

Interpro

 IPR002198; DH_sc/Rdtase_SDR.
 IPR002347; Glc/ribitol_DH.
 IPR016040; NAD(P)-bd_dom.
 IPR020904; Sc_DH/Rdtase_CS. 

Pfam

 PF00106; adh_short 

SMART

  

PROSITE

 PS00061; ADH_SHORT 

PRINTS

 PR00081; GDHRDH.
 PR00080; SDRFAMILY.