CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012366
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tubulin polyglutamylase TTLL4 
Protein Synonyms/Alias
 Tubulin--tyrosine ligase-like protein 4 
Gene Name
 TTLL4 
Gene Synonyms/Alias
 KIAA0173 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
332SDSQDPTKEIRFTEAubiquitination[1]
350LTARGFEKMPRQGCQubiquitination[1]
688MQSRFGKKEFSFFPQubiquitination[1, 2, 3]
981VTAQKMKKAYYLTQKubiquitination[1]
1062KYHGNKLKGVDLLRSubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Polyglutamylase which preferentially modifies beta- tubulin and nucleosome assembly proteins NAP1 and NAP2. Involved in the side-chain initiation step of the polyglutamylation reaction rather than in the elongation step (By similarity). 
Sequence Annotation
 DOMAIN 604 947 TTL.
 MOD_RES 691 691 Phosphoserine.
 MOD_RES 696 696 Phosphoserine.  
Keyword
 Cell projection; Cilium; Complete proteome; Cytoplasm; Cytoskeleton; Ligase; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1199 AA 
Protein Sequence
MASAGTQHYS IGLRQKNSFK QSGPSGTVPA TPPEKPSEGR VWPQAHQQVK PIWKLEKKQV 60
ETLSAGLGPG LLGVPPQPAY FFCPSTLCSS GTTAVIAGHS SSCYLHSLPD LFNSTLLYRR 120
SSYRQKPYQQ LESFCLRSSP SEKSPFSLPQ KSLPVSLTAN KATSSMVFSM AQPMASSSTE 180
PYLCLAAAGE NPSGKSLASA ISGKIPSPLS SSYKPMLNNN SFMWPNSTPV PLLQTTQGLK 240
PVSPPKIQPV SWHHSGGTGD CAPQPVDHKV PKSIGTVPAD ASAHIALSTA SSHDTSTTSV 300
ASSWYNRNNL AMRAEPLSCA LDDSSDSQDP TKEIRFTEAV RKLTARGFEK MPRQGCQLEQ 360
SSFLNPSFQW NVLNRSRRWK PPAVNQQFPQ EDAGSVRRVL PGASDTLGLD NTVFCTKRIS 420
IHLLASHASG LNHNPACESV IDSSAFGEGK APGPPFPQTL GIANVATRLS SIQLGQSEKE 480
RPEEARELDS SDRDISSATD LQPDQAETED TEEELVDGLE DCCSRDENEE EEGDSECSSL 540
SAVSPSESVA MISRSCMEIL TKPLSNHEKV VRPALIYSLF PNVPPTIYFG TRDERVEKLP 600
WEQRKLLRWK MSTVTPNIVK QTIGRSHFKI SKRNDDWLGC WGHHMKSPSF RSIREHQKLN 660
HFPGSFQIGR KDRLWRNLSR MQSRFGKKEF SFFPQSFILP QDAKLLRKAW ESSSRQKWIV 720
KPPASARGIG IQVIHKWSQL PKRRPLLVQR YLHKPYLISG SKFDLRIYVY VTSYDPLRIY 780
LFSDGLVRFA SCKYSPSMKS LGNKFMHLTN YSVNKKNAEY QANADEMACQ GHKWALKALW 840
NYLSQKGVNS DAIWEKIKDV VVKTIISSEP YVTSLLKMYV RRPYSCHELF GFDIMLDENL 900
KPWVLEVNIS PSLHSSSPLD ISIKGQMIRD LLNLAGFVLP NAEDIISSPS SCSSSTTSLP 960
TSPGDKCRMA PEHVTAQKMK KAYYLTQKIP DQDFYASVLD VLTPDDVRIL VEMEDEFSRR 1020
GQFERIFPSH ISSRYLRFFE QPRYFNILTT QWEQKYHGNK LKGVDLLRSW CYKGFHMGVV 1080
SDSAPVWSLP TSLLTISKDD VILNAFSKSE TSKLGKQSSC EVSLLLSEDG TTPKSKKTQA 1140
GLSPYPQKPS SSKDSEDTSK EPSLSTQTLP VIKCSGQTSR LSASSTFQSI SDSLLAVSP 1199 
Gene Ontology
 GO:0005929; C:cilium; IEA:UniProtKB-SubCell.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0005932; C:microtubule basal body; IEA:UniProtKB-SubCell.
 GO:0015631; F:tubulin binding; ISS:UniProtKB.
 GO:0004835; F:tubulin-tyrosine ligase activity; IEA:InterPro.
 GO:0018095; P:protein polyglutamylation; ISS:UniProtKB. 
Interpro
 IPR004344; Tub_tyr_ligase. 
Pfam
 PF03133; TTL 
SMART
  
PROSITE
 PS51221; TTL 
PRINTS