CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008583
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Methylsterol monooxygenase 
Protein Synonyms/Alias
 C-4 methylsterol oxidase 
Gene Name
 ERG25 
Gene Synonyms/Alias
 FET6; YGR060W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
86IPYFRRWKLQPTKIPubiquitination[1]
91RWKLQPTKIPSAKEQubiquitination[1]
96PTKIPSAKEQLYCLKubiquitination[1, 2, 3, 4, 5]
289TESGPEAKASREERMubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery.
 Hitchcock AL, Auld K, Gygi SP, Silver PA.
 Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12735-40. [PMID: 14557538]
 [3] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [4] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269]
 [5] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 Catalyzes the first step in the removal of the two C-4 methyl groups of 4,4-dimethylzymosterol. 
Sequence Annotation
 MOTIF 160 164 Histidine box-1.
 MOTIF 173 177 Histidine box-2.
 MOTIF 257 263 Histidine box-3.
 CROSSLNK 96 96 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Complete proteome; Endoplasmic reticulum; Iron; Isopeptide bond; Lipid biosynthesis; Lipid metabolism; Membrane; NAD; Oxidoreductase; Reference proteome; Steroid biosynthesis; Steroid metabolism; Sterol biosynthesis; Sterol metabolism; Transmembrane; Transmembrane helix; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 309 AA 
Protein Sequence
MSAVFNNATL SGLVQASTYS QTLQNVAHYQ PQLNFMEKYW AAWYSYMNND VLATGLMFFL 60
LHEFMYFFRC LPWFIIDQIP YFRRWKLQPT KIPSAKEQLY CLKSVLLSHF LVEAIPIWTF 120
HPMCEKLGIT VEVPFPSLKT MALEIGLFFV LEDTWHYWAH RLFHYGVFYK YIHKQHHRYA 180
APFGLSAEYA HPAETLSLGF GTVGMPILYV MYTGKLHLFT LCVWITLRLF QAVDSHSGYD 240
FPWSLNKIMP FWAGAEHHDL HHHYFIGNYA SSFRWWDYCL DTESGPEAKA SREERMKKRA 300
ENNAQKKTN 309 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; IDA:SGD.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0000254; F:C-4 methylsterol oxidase activity; IDA:SGD.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0006696; P:ergosterol biosynthetic process; IDA:SGD.
 GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro. 
Interpro
 IPR006694; Fatty_acid_hydroxylase. 
Pfam
 PF04116; FA_hydroxylase 
SMART
  
PROSITE
  
PRINTS