UniProt Accession

 MARCS_HUMAN; P29966; E1P560; Q2LA83; Q5TDB7 

Genbank Protein ID

 M68956; M68955; D10522; DQ341274; AL132660; CH471051; CH471051; BC089040 

Genbank Nucleotide ID

 AAA59555.1; AAA59554.1; BAA01392.1; ABC67467.1; CAI19942.1; EAW48258.1; EAW48259.1; AAH89040.1 

Protein Name

 Myristoylated alanine-rich C-kinase substrate 

Protein Synonyms/Alias

 MARCKS; Protein kinase C substrate, 80 kDa protein, light chain; 80K-L protein; PKCSL 

Gene Name

 MARCKS 

Gene Synonyms/Alias

 MACS; PRKCSL 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
11	QFSKTAAKGEAAAER	ubiquitination	[1, 2]
55	AAAESGAKEELQANG	ubiquitination	[1, 2]
69	GSAPAADKEEPAAAG	ubiquitination	[1, 2]
87	ASPSAAEKGEPAAAA	ubiquitination	[1, 2, 3]
105	AGASPVEKEAPAEGE	ubiquitination	[1, 2]
152	SPSNETPKKKKKRFS	ubiquitination	[1]
165	FSFKKSFKLSGFSFK	ubiquitination	[4, 5]
172	KLSGFSFKKNKKEAG	acetylation	[6]
172	KLSGFSFKKNKKEAG	ubiquitination	[2, 4, 5]
176	FSFKKNKKEAGEGGE	ubiquitination	[1]
263	KAAEEPSKVEEKKAE	ubiquitination	[2]
267	EPSKVEEKKAEEAGA	ubiquitination	[2]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome.
 Morselli E, Mariño G, Bennetzen MV, Eisenberg T, Megalou E, Schroeder S, Cabrera S, Bénit P, Rustin P, Criollo A, Kepp O, Galluzzi L, Shen S, Malik SA, Maiuri MC, Horio Y, López-Otín C, Andersen JS, Tavernarakis N, Madeo F, Kroemer G.
 J Cell Biol. 2011 Feb 21;192(4):615-29. [PMID: 21339330] 

Functional Description

 MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. 

Sequence Annotation

 REGION 152 176 Calmodulin-binding (PSD).
 MOD_RES 27 27 Phosphoserine.
 MOD_RES 46 46 Phosphoserine.
 MOD_RES 63 63 Phosphoserine.
 MOD_RES 77 77 Phosphoserine.
 MOD_RES 81 81 Phosphoserine.
 MOD_RES 101 101 Phosphoserine.
 MOD_RES 118 118 Phosphoserine.
 MOD_RES 135 135 Phosphoserine (By similarity).
 MOD_RES 143 143 Phosphothreonine.
 MOD_RES 145 145 Phosphoserine.
 MOD_RES 147 147 Phosphoserine (By similarity).
 MOD_RES 150 150 Phosphothreonine.
 MOD_RES 159 159 Phosphoserine; by PKC.
 MOD_RES 163 163 Phosphoserine; by PKC.
 MOD_RES 167 167 Phosphoserine; by PKC.
 MOD_RES 170 170 Phosphoserine; by PKC.
 MOD_RES 262 262 Phosphoserine (By similarity).
 LIPID 2 2 N-myristoyl glycine (By similarity).  

Keyword

 Actin-binding; Calmodulin-binding; Complete proteome; Cytoplasm; Cytoskeleton; Lipoprotein; Membrane; Myristate; Phosphoprotein; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 332 AA 

Protein Sequence

Gene Ontology

 GO:0015629; C:actin cytoskeleton; TAS:ProtInc.
 GO:0005938; C:cell cortex; IEA:Compara.
 GO:0005813; C:centrosome; IEA:Compara.
 GO:0042585; C:germinal vesicle; IEA:Compara.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0051015; F:actin filament binding; TAS:ProtInc.
 GO:0005516; F:calmodulin binding; TAS:ProtInc.
 GO:0006112; P:energy reserve metabolic process; TAS:Reactome.
 GO:0050796; P:regulation of insulin secretion; TAS:Reactome.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 

Interpro

 IPR002101; MARCKS. 

Pfam

 PF02063; MARCKS 

SMART

  

PROSITE

 PS00826; MARCKS_1
 PS00827; MARCKS_2 

PRINTS

 PR00963; MARCKS.