CPLM-018562

Genbank Nucleotide ID

Fructose-bisphosphate aldolase B

Protein Synonyms/Alias

Aldolase 2; Liver-type aldolase

Mus musculus (Mouse)

Position	Peptide	Type	References
13	PALTPEQKKELSEIA	acetylation	[1]
13	PALTPEQKKELSEIA	succinylation	[1]
13	PALTPEQKKELSEIA	ubiquitination	[2]
14	ALTPEQKKELSEIAQ	acetylation	[3, 4]
14	ALTPEQKKELSEIAQ	ubiquitination	[2]
28	QRIVANGKGILAADE	ubiquitination	[2]
48	GNRLQRIKVENTEEN	acetylation	[5, 6]
48	GNRLQRIKVENTEEN	ubiquitination	[2]
99	NLFRNVLKEKGIVVG	acetylation	[5, 6]
101	FRNVLKEKGIVVGIK	ubiquitination	[2]
108	KGIVVGIKLDQGGAP	acetylation	[3]
108	KGIVVGIKLDQGGAP	ubiquitination	[2]
121	APLAGTNKETTIQGL	acetylation	[1, 3, 4, 5]
121	APLAGTNKETTIQGL	succinylation	[1]
121	APLAGTNKETTIQGL	ubiquitination	[2]
140	ERCAQYKKDGVDFGK	ubiquitination	[2]
147	KDGVDFGKWRAVLRI	acetylation	[3, 5, 6, 7]
147	KDGVDFGKWRAVLRI	phosphoglycerylation	[8]
147	KDGVDFGKWRAVLRI	ubiquitination	[2]
230	YLEGTLLKPNMVTAG	acetylation	[5]
230	YLEGTLLKPNMVTAG	ubiquitination	[2]
242	TAGHACTKKYTPEQV	ubiquitination	[2]
243	AGHACTKKYTPEQVA	phosphoglycerylation	[8]
243	AGHACTKKYTPEQVA	ubiquitination	[2]
317	ALAAWGGKAANKKAT	acetylation	[1]
317	ALAAWGGKAANKKAT	succinylation	[1]
317	ALAAWGGKAANKKAT	ubiquitination	[2]
322	GGKAANKKATQEAFM	ubiquitination	[2]
330	ATQEAFMKRAMANCQ	acetylation	[1, 5, 9]
330	ATQEAFMKRAMANCQ	succinylation	[1]
330	ATQEAFMKRAMANCQ	ubiquitination	[2]

[1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
[2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
[3] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
Simon GM, Cheng J, Gordon JI.
Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
[4] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
[5] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
[6] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
[7] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
[8] Functional lysine modification by an intrinsically reactive primary glycolytic metabolite.
Moellering RE, Cravatt BF.
Science. 2013 Aug 2;341(6145):549-53. [PMID: 23908237]
[9] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]

Functional Description

ACT_SITE 188 188 Proton acceptor (By similarity).
ACT_SITE 230 230 Schiff-base intermediate with
BINDING 56 56 Substrate.
BINDING 147 147 Substrate.
MOD_RES 2 2 N-acetylalanine.
MOD_RES 36 36 Phosphoserine.

Acetylation; Complete proteome; Direct protein sequencing; Glycolysis; Lyase; Phosphoprotein; Reference proteome; Schiff base.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0034451; C:centriolar satellite; IEA:Compara.
GO:0070061; F:fructose binding; IEA:Compara.
GO:0004332; F:fructose-bisphosphate aldolase activity; IEA:EC.
GO:0030388; P:fructose 1,6-bisphosphate metabolic process; IEA:Compara.
GO:0006000; P:fructose metabolic process; IEA:Compara.
GO:0006096; P:glycolysis; IEA:UniProtKB-UniPathway.
GO:0006116; P:NADH oxidation; IEA:Compara.
GO:0032781; P:positive regulation of ATPase activity; IEA:Compara.
GO:0070072; P:vacuolar proton-transporting V-type ATPase complex assembly; IEA:Compara.

IPR000741; Aldolase_I.
IPR013785; Aldolase_TIM.

PS00158; ALDOLASE_CLASS_I