CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012459
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Squamous cell carcinoma antigen recognized by T-cells 3 
Protein Synonyms/Alias
 SART-3; hSART-3; Tat-interacting protein of 110 kDa; Tip110 
Gene Name
 SART3 
Gene Synonyms/Alias
 KIAA0156; TIP110 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
130RLEGELTKVRMARQKubiquitination[1]
294KALQQLEKYKPYEEAubiquitination[1, 2, 3]
435LRRRVDFKQDSSKELubiquitination[1]
491RLCNNMQKARELWDSubiquitination[1]
568DWDIAVQKTETRLARubiquitination[1, 4]
586QRMKAAEKEAALVQQubiquitination[1]
867KMDGMTIKENIIKVAubiquitination[1]
958MSNADFAKLFLRK**ubiquitination[2, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Regulates Tat transactivation activity through direct interaction. May be a cellular factor for HIV-1 gene expression and viral replication. 
Sequence Annotation
 REPEAT 126 158 HAT 1.
 REPEAT 164 195 HAT 2.
 REPEAT 201 237 HAT 3.
 REPEAT 242 275 HAT 4.
 REPEAT 324 356 HAT 5.
 REPEAT 359 391 HAT 6.
 REPEAT 394 430 HAT 7.
 REPEAT 487 520 HAT 8.
 DOMAIN 704 782 RRM 1.
 DOMAIN 801 878 RRM 2.
 REGION 600 670 Required for nuclear localization.
 MOTIF 601 617 Nuclear localization signal (Potential).
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 10 10 Phosphoserine.
 MOD_RES 16 16 Phosphoserine.
 MOD_RES 852 852 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Direct protein sequencing; Disease mutation; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 963 AA 
Protein Sequence
MATAAETSAS EPEAESKAGP KADGEEDEVK AARTRRKVLS RAVAAATYKT MGPAWDQQEE 60
GVSESDGDEY AMASSAESSP GEYEWEYDEE EEKNQLEIER LEEQLSINVY DYNCHVDLIR 120
LLRLEGELTK VRMARQKMSE IFPLTEELWL EWLHDEISMA QDGLDREHVY DLFEKAVKDY 180
ICPNIWLEYG QYSVGGIGQK GGLEKVRSVF ERALSSVGLH MTKGLALWEA YREFESAIVE 240
AARLEKVHSL FRRQLAIPLY DMEATFAEYE EWSEDPIPES VIQNYNKALQ QLEKYKPYEE 300
ALLQAEAPRL AEYQAYIDFE MKIGDPARIQ LIFERALVEN CLVPDLWIRY SQYLDRQLKV 360
KDLVLSVHNR AIRNCPWTVA LWSRYLLAME RHGVDHQVIS VTFEKALNAG FIQATDYVEI 420
WQAYLDYLRR RVDFKQDSSK ELEELRAAFT RALEYLKQEV EERFNESGDP SCVIMQNWAR 480
IEARLCNNMQ KARELWDSIM TRGNAKYANM WLEYYNLERA HGDTQHCRKA LHRAVQCTSD 540
YPEHVCEVLL TMERTEGSLE DWDIAVQKTE TRLARVNEQR MKAAEKEAAL VQQEEEKAEQ 600
RKRARAEKKA LKKKKKIRGP EKRGADEDDE KEWGDDEEEQ PSKRRRVENS IPAAGETQNV 660
EVAAGPAGKC AAVDVEPPSK QKEKAASLKR DMPKVLHDSS KDSITVFVSN LPYSMQEPDT 720
KLRPLFEACG EVVQIRPIFS NRGDFRGYCY VEFKEEKSAL QALEMDRKSV EGRPMFVSPC 780
VDKSKNPDFK VFRYSTSLEK HKLFISGLPF SCTKEELEEI CKAHGTVKDL RLVTNRAGKP 840
KGLAYVEYEN ESQASQAVMK MDGMTIKENI IKVAISNPPQ RKVPEKPETR KAPGGPMLLP 900
QTYGARGKGR TQLSLLPRAL QRPSAAAPQA ENGPAAAPAV AAPAATEAPK MSNADFAKLF 960
LRK 963 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA.
 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0006396; P:RNA processing; IEA:InterPro. 
Interpro
 IPR003107; HAT.
 IPR008669; LSM_interact.
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom. 
Pfam
 PF05391; Lsm_interact
 PF00076; RRM_1 
SMART
 SM00386; HAT
 SM00360; RRM 
PROSITE
 PS50102; RRM 
PRINTS