CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004656
UniProt Accession
Genbank Protein ID
 D90109 
Genbank Nucleotide ID
Protein Name
 Long-chain-fatty-acid--CoA ligase 1 
Protein Synonyms/Alias
 Long-chain acyl-CoA synthetase 1; LACS 1; Long-chain-fatty-acid--CoA ligase, liver isozyme 
Gene Name
 Acsl1 
Gene Synonyms/Alias
 Acs1; Acsl2; Facl2 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
203LSVIFADKPEKAKLLacetylation[1]
357RLLMDDLKVLQPTIFacetylation[1]
387GQANTSVKRWLLDFAacetylation[1]
415RNNSLWDKLIFHKIQacetylation[1]
420WDKLIFHKIQSSLGGacetylation[1]
518VKGANVFKGYLKDPAacetylation[1]
522NVFKGYLKDPARTAEacetylation[1]
533RTAEALDKDGWLHTGacetylation[1]
544LHTGDIGKWLPNGTLacetylation[1, 2]
573GEYIAPEKIENIYLRacetylation[1]
633CRNKDINKAILEDMVacetylation[1, 2, 3]
641AILEDMVKLGKNAGLacetylation[1]
644EDMVKLGKNAGLKPFacetylation[1]
649LGKNAGLKPFEQVKGacetylation[1]
676GLLTPTLKAKRPELRacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Proteomics of mitochondrial inner and outer membranes.
 Distler AM, Kerner J, Hoppel CL.
 Proteomics. 2008 Oct;8(19):4066-82. [PMID: 18763707]
 [3] Post-translational modifications of rat liver mitochondrial outer membrane proteins identified by mass spectrometry.
 Distler AM, Kerner J, Hoppel CL.
 Biochim Biophys Acta. 2007 May;1774(5):628-36. [PMID: 17478130] 
Functional Description
 Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses oleate, arachidonate, eicosapentaenoate and docosahexaenoate as substrates. 
Sequence Annotation
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 9 9 Nitrated tyrosine.
 MOD_RES 85 85 Phosphotyrosine.
 MOD_RES 86 86 Nitrated tyrosine.
 MOD_RES 544 544 N6-acetyllysine (By similarity).
 MOD_RES 633 633 N6-acetyllysine.  
Keyword
 Acetylation; ATP-binding; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Fatty acid metabolism; Ligase; Lipid metabolism; Magnesium; Membrane; Microsome; Mitochondrion; Mitochondrion outer membrane; Nitration; Nucleotide-binding; Peroxisome; Phosphoprotein; Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 699 AA 
Protein Sequence
MEVHELFRYF RMPELIDIRQ YVRTLPTNTL MGFGAFAALT TFWYATRPKA LKPPCDLSMQ 60
SVEVTGTTEG VRRSAVLEDD KLLLYYYDDV RTMYDGFQRG IQVSNDGPCL GSRKPNQPYE 120
WISYKQVAEM AECIGSALIQ KGFKPCSEQF IGIFSQNRPE WVTIEQGCFT YSMVVVPLYD 180
TLGTDAITYI VNKAELSVIF ADKPEKAKLL LEGVENKLTP CLKIIVIMDS YDNDLVERGQ 240
KCGVEIIGLK ALEDLGRVNR TKPKPPEPED LAIICFTSGT TGNPKGAMVT HQNIMNDCSG 300
FIKATESAFI ASPEDVLISF LPLAHMFETV VECVMLCHGA KIGFFQGDIR LLMDDLKVLQ 360
PTIFPVVPRL LNRMFDRIFG QANTSVKRWL LDFASKRKEA ELRSGIVRNN SLWDKLIFHK 420
IQSSLGGKVR LMITGAAPVS ATVLTFLRAA LGCQFYEGYG QTECTAGCCL SLPGDWTAGH 480
VGAPMPCNYI KLVDVEDMNY QAAKGEGEVC VKGANVFKGY LKDPARTAEA LDKDGWLHTG 540
DIGKWLPNGT LKIIDRKKHI FKLAQGEYIA PEKIENIYLR SEAVAQVFVH GESLQAFLIA 600
IVVPDVEILP SWAQKRGFQG SFEELCRNKD INKAILEDMV KLGKNAGLKP FEQVKGIAVH 660
PELFSIDNGL LTPTLKAKRP ELRNYFRSQI DELYSTIKI 699 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; IDA:RGD.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005741; C:mitochondrial outer membrane; IDA:RGD.
 GO:0005778; C:peroxisomal membrane; IDA:HGNC.
 GO:0005886; C:plasma membrane; IDA:RGD.
 GO:0003987; F:acetate-CoA ligase activity; TAS:RGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004467; F:long-chain fatty acid-CoA ligase activity; IDA:UniProtKB.
 GO:0033211; P:adiponectin-mediated signaling pathway; IEA:Compara.
 GO:0015908; P:fatty acid transport; IGI:RGD.
 GO:0008610; P:lipid biosynthetic process; IEA:Compara.
 GO:0044539; P:long-chain fatty acid import; IEA:Compara.
 GO:0071902; P:positive regulation of protein serine/threonine kinase activity; IEA:Compara.
 GO:0042493; P:response to drug; IEP:RGD.
 GO:0007584; P:response to nutrient; IEP:RGD.
 GO:0034201; P:response to oleic acid; IEP:RGD.
 GO:0014070; P:response to organic cyclic compound; IEP:RGD.
 GO:0006641; P:triglyceride metabolic process; IGI:RGD.
 GO:0042178; P:xenobiotic catabolic process; IDA:RGD. 
Interpro
 IPR020845; AMP-binding_CS.
 IPR000873; AMP-dep_Synth/Lig. 
Pfam
 PF00501; AMP-binding 
SMART
  
PROSITE
 PS00455; AMP_BINDING 
PRINTS