UniProt Accession

 SHRPN_MOUSE; Q91WA6; Q7TNT3; Q8CHL3 

Genbank Protein ID

 AB052763; BC016203; BC055758 

Genbank Nucleotide ID

 BAC53796.1; AAH16203.1; AAH55758.1 

Protein Name

 Sharpin 

Protein Synonyms/Alias

 Shank-associated RH domain-interacting protein; Shank-interacting protein-like 1; mSIPL1 

Gene Name

 Sharpin 

Gene Synonyms/Alias

 Cpdm; Sipl1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
312	GQSLQNSKMDRKLGL	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. 

Sequence Annotation

 DOMAIN 216 285 Ubiquitin-like.
 ZN_FING 342 371 RanBP2-type.
 REGION 1 177 Self-association (By similarity).
 REGION 172 305 Interaction with SHANK1 (By similarity).
 MOD_RES 163 163 Phosphoserine (By similarity).
 MOD_RES 307 307 Phosphoserine (By similarity).  

Keyword

 Alternative splicing; Complete proteome; Cytoplasm; Metal-binding; Phosphoprotein; Reference proteome; Ubl conjugation pathway; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 380 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; ISS:UniProtKB.
 GO:0071797; C:LUBAC complex; ISS:UniProtKB.
 GO:0014069; C:postsynaptic density; IEA:Compara.
 GO:0031593; F:polyubiquitin binding; ISS:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0030262; P:apoptotic nuclear changes; IMP:MGI.
 GO:0007420; P:brain development; IEA:Compara.
 GO:0031424; P:keratinization; IMP:MGI.
 GO:0007005; P:mitochondrion organization; IMP:MGI.
 GO:0050728; P:negative regulation of inflammatory response; IMP:UniProtKB.
 GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB cascade; IMP:UniProtKB.
 GO:0051260; P:protein homooligomerization; IEA:Compara.
 GO:0097039; P:protein linear polyubiquitination; ISS:UniProtKB.
 GO:2000348; P:regulation of CD40 signaling pathway; ISS:UniProtKB.
 GO:0010803; P:regulation of tumor necrosis factor-mediated signaling pathway; IMP:UniProtKB. 

Interpro

 IPR026168; SHARPIN.
 IPR001876; Znf_RanBP2. 

Pfam

 PF00641; zf-RanBP 

SMART

 SM00547; ZnF_RBZ 

PROSITE

 PS50053; UBIQUITIN_2
 PS01358; ZF_RANBP2_1
 PS50199; ZF_RANBP2_2 

PRINTS