CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001465
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2 
Protein Synonyms/Alias
 D-fructose-6-phosphate amidotransferase 2; Glutamine:fructose-6-phosphate amidotransferase 2; GFAT 2; GFAT2; Hexosephosphate aminotransferase 2 
Gene Name
 GFPT2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
50DGNNHEVKERHIQLVubiquitination[1]
136LRKFLESKGYEFESEubiquitination[1]
328NFSAFMQKEIFEQPEubiquitination[1]
677DFPRNLAKSVTVE**ubiquitination[1, 2, 3]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. 
Sequence Annotation
 DOMAIN 2 288 Glutamine amidotransferase type-2.
 DOMAIN 360 499 SIS 1.
 DOMAIN 531 672 SIS 2.
 ACT_SITE 2 2 For GATase activity (By similarity).
 MOD_RES 245 245 Phosphoserine (By similarity).  
Keyword
 Aminotransferase; Complete proteome; Glutamine amidotransferase; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 682 AA 
Protein Sequence
MCGIFAYMNY RVPRTRKEIF ETLIKGLQRL EYRGYDSAGV AIDGNNHEVK ERHIQLVKKR 60
GKVKALDEEL YKQDSMDLKV EFETHFGIAH TRWATHGVPS AVNSHPQRSD KGNEFVVIHN 120
GIITNYKDLR KFLESKGYEF ESETDTETIA KLIKYVFDNR ETEDITFSTL VERVIQQLEG 180
AFALVFKSVH YPGEAVATRR GSPLLIGVRS KYKLSTEQIP ILYRTCTLEN VKNICKTRMK 240
RLDSSACLHA VGDKAVEFFF ASDASAIIEH TNRVIFLEDD DIAAVADGKL SIHRVKRSAS 300
DDPSRAIQTL QMELQQIMKG NFSAFMQKEI FEQPESVFNT MRGRVNFETN TVLLGGLKDH 360
LKEIRRCRRL IVIGCGTSYH AAVATRQVLE ELTELPVMVE LASDFLDRNT PVFRDDVCFF 420
ISQSGETADT LLALRYCKDR GALTVGVTNT VGSSISRETD CGVHINAGPE IGVASTKAYT 480
SQFISLVMFG LMMSEDRISL QNRRQEIIRG LRSLPELIKE VLSLEEKIHD LALELYTQRS 540
LLVMGRGYNY ATCLEGALKI KEITYMHSEG ILAGELKHGP LALIDKQMPV IMVIMKDPCF 600
AKCQNALQQV TARQGRPIIL CSKDDTESSK FAYKTIELPH TVDCLQGILS VIPLQLLSFH 660
LAVLRGYDVD FPRNLAKSVT VE 682 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0030246; F:carbohydrate binding; IEA:InterPro.
 GO:0004360; F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity; TAS:ProtInc.
 GO:0016051; P:carbohydrate biosynthetic process; IEA:InterPro.
 GO:0006488; P:dolichol-linked oligosaccharide biosynthetic process; TAS:Reactome.
 GO:0006112; P:energy reserve metabolic process; TAS:ProtInc.
 GO:0006002; P:fructose 6-phosphate metabolic process; TAS:ProtInc.
 GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
 GO:0043687; P:post-translational protein modification; TAS:Reactome.
 GO:0018279; P:protein N-linked glycosylation via asparagine; TAS:Reactome.
 GO:0006048; P:UDP-N-acetylglucosamine biosynthetic process; TAS:Reactome. 
Interpro
 IPR017932; GATase_2_dom.
 IPR000583; GATase_dom.
 IPR005855; GlmS_trans.
 IPR001347; SIS. 
Pfam
 PF00310; GATase_2
 PF01380; SIS 
SMART
  
PROSITE
 PS51278; GATASE_TYPE_2
 PS51464; SIS 
PRINTS