CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008816
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 IST1 homolog 
Protein Synonyms/Alias
 hIST1; Putative MAPK-activating protein PM28 
Gene Name
 IST1 
Gene Synonyms/Alias
 KIAA0174 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
7*MLGSGFKAERLRVNubiquitination[1, 2, 3]
48ADYLAAGKDERARIRubiquitination[1, 3, 4]
118QSEVAELKIVADQLCubiquitination[3]
127VADQLCAKYSKEYGKubiquitination[2, 3]
134KYSKEYGKLCRTNQIubiquitination[2, 3]
151VNDRLMHKLSVEAPPubiquitination[3, 4]
159LSVEAPPKILVERYLubiquitination[3, 5, 6]
205VGFTDDVKKGGPGRGubiquitination[1, 4]
206GFTDDVKKGGPGRGGubiquitination[4]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Proposed to be involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. 
Sequence Annotation
 REGION 1 168 Interaction with CHMP1A and CHMP1B.
 REGION 64 279 Interaction with VPS37B.
 REGION 190 364 Interaction with VTA1.
 REGION 348 364 Interaction with VPS4A, VTA1, MITD1
 MOTIF 321 332 Type-2 MIT-interacting motif.
 MOTIF 351 361 MIT-interacting motif.  
Keyword
 3D-structure; Alternative splicing; Cell cycle; Cell division; Complete proteome; Cytoplasmic vesicle; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 364 AA 
Protein Sequence
MLGSGFKAER LRVNLRLVIN RLKLLEKKKT ELAQKARKEI ADYLAAGKDE RARIRVEHII 60
REDYLVEAME ILELYCDLLL ARFGLIQSMK ELDSGLAESV STLIWAAPRL QSEVAELKIV 120
ADQLCAKYSK EYGKLCRTNQ IGTVNDRLMH KLSVEAPPKI LVERYLIEIA KNYNVPYEPD 180
SVVMAEAPPG VETDLIDVGF TDDVKKGGPG RGGSGGFTAP VGGPDGTVPM PMPMPMPSAN 240
TPFSYPLPKG PSDFNGLPMG TYQAFPNIHP PQIPATPPSY ESVDDINADK NISSAQIVGP 300
GPKPEASAKL PSRPADNYDN FVLPELPSVP DTLPTASAGA STSASEDIDF DDLSRRFEEL 360
KKKT 364 
Gene Ontology
 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0016023; C:cytoplasmic membrane-bounded vesicle; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; IDA:UniProtKB.
 GO:0070062; C:extracellular vesicular exosome; IDA:UniProtKB.
 GO:0090543; C:Flemming body; IDA:UniProtKB.
 GO:0032403; F:protein complex binding; IDA:UniProtKB.
 GO:0009838; P:abscission; IDA:UniProtKB.
 GO:0000910; P:cytokinesis; IMP:UniProtKB.
 GO:0045862; P:positive regulation of proteolysis; IDA:UniProtKB.
 GO:0008104; P:protein localization; IMP:UniProtKB.
 GO:0019076; P:viral release from host cell; IDA:UniProtKB.
 GO:0046746; P:virus budding from nuclear membrane by viral capsid re-envelopment; IDA:UniProtKB. 
Interpro
 IPR005061; DUF292_euk. 
Pfam
 PF03398; Ist1 
SMART
  
PROSITE
  
PRINTS